UTY (NM_001258259) Human Tagged ORF Clone

CAT#: RG235269

UTY (GFP-tagged) - Homo sapiens ubiquitously transcribed tetratricopeptide repeat containing, Y-linked (UTY), transcript variant 14

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: UTY
• Synonyms: KDM6AL; KDM6C; UTY1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG235269 representing NM_001258259
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAATCCTGCGCAGTGTCGCTCACTACCGCCGCTGTTGCCTTCGGTGATGAGGCAAAGAAAATGGCGG
  AAGGAAAAGCGAGCCGCGAGAGTGAAGAGGAGTCTGTTAGCCTGACAGTCGAGGAAAGGGAGGCGCTTGG
  TGGCATGGACAGCCGTCTCTTCGGGTTCGTGAGGCTTCATGAAGATGGCGCCAGAACGAAGACCCTACTA
  GGCAAGGCTGTTCGCTGCTACGAATCTTTAATCTTAAAAGCTGAAGGAAAAGTGGAGTCTGACTTCTTTT
  GCCAATTAGGTCACTTCAACCTCTTGTTGGAAGATTATTCAAAAGCATTATCTGCATATCAGAGATATTA
  CAGTTTACAGGCTGACTACTGGAAGAATGCTGCGTTTTTATATGGCCTTGGTTTGGTCTACTTCTACTAC
  AATGCATTTCATTGGGCAATTAAAGCATTTCAAGATGTCCTTTATGTTGACCCCAGCTTTTGTCGAGCCA
  AGGAAATTCATTTACGACTTGGGCTCATGTTCAAAGTGAACACAGACTACAAGTCTAGTTTAAAGCATTT
  TCAGTTAGCCTTGATTGACTGTAATCCATGTACTTTGTCCAATGCTGAAATTCAATTTCATATTGCCCAT
  TTGTATGAAACCCAGAGGAAGTATCATTCTGCAAAGGAGGCATATGAACAACTTTTGCAGACAGAAAACC
  TTCCTGCACAAGTAAAAGCAACTGTATTGCAACAGTTAGGTTGGATGCATCATAATATGGATCTAGTAGG
  AGACAAAGCCACAAAGGAAAGCTATGCTATTCAGTATCTCCAAAAGTCTTTGGAGGCAGATCCTAATTCT
  GGCCAATCGTGGTATTTTCTTGGAAGGCAATCTATTGATAAATCAGAAGCAAGTGCAGATACATGGTGTT
  CAATAGGTGTGTTGTATCAGCAGCAAAATCAGCCTATGGATGCTTTACAGGCATATATTTGTGCTGTACA
  ATTGGACCATGGGCATGCCGCAGCCTGGATGGACCTAGGTACTCTCTATGAATCCTGCAATCAACCTCAA
  GATGCCATTAAATGCTACCTAAATGCAGCTAGAAGCAAACGTTGTAGTAATACCTCTACGCTTGCTGCAA
  GAATTAAATTTCTACAGAATGGTTCTGATAACTGGAATGGTGGCCAGAGTCTTTCACATCATCCAGTACA
  GCAAGTTTATTCGTTGTGTTTGACACCACAGAAATTACAGCACTTGGAACAACTGCGAGCAAATAGAGAT
  AATTTAAATCCAGCACAGAAGCATCAGCTGGAACAGTTAGAAAGTCAGTTTGTCTTAATGCAGCAAATGA
  GACACAAAGAAGTTGCTCAGGTACGAACTACTGGAATTCATAACGGGGCCATAACTGATTCATCACTGCC
  TACAAACTCTGTCTCTAATCGACAACCACATGGTGCTCTGACCAGAGTATCTAGCGTCTCTCAGCCTGGA
  GTTCGCCCTGCTTGTGTTGAAAAACTTTTGTCCAGTGGAGCTTTTTCTGCAGGCTGTATTCCTTGTGGCA
  CATCAAAAATTCTAGGAAGTACAGACACTATCTTGCTAGGCAGTAATTGTATAGCAGGAAGTGAAAGTAA
  TGGAAATGTGCCTTACCTGCAGCAAAATACACACACTCTACCTCATAATCATACAGACCTGAACAGCAGC
  ACAGAAGAGCCATGGAGAAAACAGCTATCTAACTCCGCTCAGGGGCTTCATAAAAGTCAGAGTTCATGTT
  TGTCAGGACCTAATGAAGAACAACCTCTGTTTTCCACTGGGTCAGCCCAGTATCACCAGGCAACTAGCAC
  TGGTATTAAGAAGGCGAATGAACATCTCACTCTGCCTAGTAATTCAGTACCACAGGGGGATGCTGACAGT
  CACCTCTCCTGTCATACTGCTACCTCAGGTGGACAACAAGGCATTATGTTTACCAAAGAGAGCAAGCCTT
  CAAAAAATAGATCCTTGGTGCCTGAAACAAGCAGGCATACTGGAGACACATCTAATGGCTGTGCTGATGT
  CAAGGGACTTTCTAATCATGTTCATCAGTTGATAGCAGATGCTGTTTCCAGTCCTAACCATGGAGATTCA
  CCAAATTTATTAATTGCAGACAATCCTCAGCTCTCTGCTTTGTTGATTGGAAAAGCCAATGGCAATGTGG
  GTACTGGAACCTGTGACAAAGTGAATAATATTCACCCAGCTGTTCATACAAAGACTGATCATTCTGTTGC
  CTCTTCACCCTCTTCAGCCATTTCCACAGCAACACCTTCTCCTAAATCCACTGAGCAGAGAAGCATAAAC
  AGTGTTACCAGCCTTAACAGTCCTCACAGTGGATTACACACAGTCAATGGAGAGGGGCTGGGGAAGTCAC
  AGAGCTCTACAAAAGTAGACCTGCCTTTAGCTAGCCACAGATCTACTTCTCAGATCTTACCATCAATGTC
  AGTGTCTATATGCCCCAGTTCAACAGAAGTTCTGAAAGCATGCAGGAATCCAGGTAAAAATGGCTTGTCT
  AATAGCTGCATTTTGTTAGATAAATGTCCACCTCCAAGACCACCAACTTCACCATACCCACCCTTGCCAA
  AGGACAAGTTGAATCCACCCACACCTAGTATTTACTTGGAAAATAAACGTGATGCTTTCTTTCCTCCATT
  ACATCAATTTTGTACAAATCCAAAAAACCCTGTTACAGTAATACGTGGCCTTGCTGGAGCTCTTAAATTA
  GATCTTGGACTTTTCTCTACCAAAACTTTGGTAGAAGCTAACAATGAACATATGGTAGAAGTGAGGACAC
  AGTTGCTGCAACCAGCAGATGAAAACTGGGATCCCACTGGAACAAAGAAAATCTGGCGTTGTGAAAGCAA
  TAGATCTCATACTACAATTGCCAAATACGCACAATACCAGGCTTCCTCCTTCCAGGAATCATTGAGAGAA
  GAAAATGAGAAAAGAACACAACACAAAGATCATTCAGATAACGAATCCACATCTTCAGAGAATTCTGGAA
  GGAGAAGGAAAGGACCTTTTAAAACCATAAAATTTGGGACCAACATTGACCTCTCTGATAACAAAAAGTG
  GAAGTTGCAGTTACATGAACTGACTAAACTTCCTGCTTTTGCGCGTGTGGTGTCAGCAGGAAATCTTCTA
  ACCCATGTTGGGCATACCATTCTGGGCATGAATACAGTACAACTGTATATGAAAGTTCCAGGGAGTCGGA
  CACCAGGTCACCAAGAAAATAACAACTTCTGCTCTGTTAACATAAATATTGGTCCAGGAGATTGTGAATG
  GTTTGTTGTACCTGAAGATTATTGGGGTGTTCTGAATGACTTCTGTGAAAAAAATAATTTGAATTTTTTA
  ATGAGTTCTTGGTGGCCCAACCTTGAAGATCTTTATGAAGCAAATGTCCCTGTGTATAGATTTATTCAGC
  GACCTGGAGATTTGGTCTGGATAAATGCAGGCACTGTGCATTGGGTTCAAGCTGTTGGCTGGTGCAATAA
  CATTGCCTGGAATGTTGGTCCACTTACAGCCTGCCAGTATAAATTGGCAGTGGAACGGTATGAATGGAAC
  AAATTGAAAAGTGTGAAGTCACCAGTACCCATGGTGCATCTTTCCTGGAATATGGCACGAAATATCAAAG
  TCTCAGATCCAAAGCTTTTTGAAATGATTAAGTATTGTCTTTTGAAAATTCTGAAGCAATATCAGACATT
  GAGAGAAGCTCTTGTTGCAGCAGGAAAAGAGGTTATATGGCATGGGCGGACAAATGATGAACCAGCTCAT
  TACTGTAGCATTTGTGAGGTGGAGGTTTTTAATCTGCTTTTTGTCACTAATGAAAGCAATACTCAAAAAA
  CCTACATAGTACATTGCCATGATTGTGCACGAAAAACAAGCAAAAGTTTGGAAAATTTTGTGGTGCTCGA
  ACAGTACAAAATGGAGGACCTAATCCAAGTTTATGATCAATTTACACTAGCTCTTTCATTATCATCCTCA
  TCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG235269 representing NM_001258259
  Red=Cloning site Green=Tags(s)
  
  MKSCAVSLTTAAVAFGDEAKKMAEGKASRESEEESVSLTVEEREALGGMDSRLFGFVRLHEDGARTKTLL
  GKAVRCYESLILKAEGKVESDFFCQLGHFNLLLEDYSKALSAYQRYYSLQADYWKNAAFLYGLGLVYFYY
  NAFHWAIKAFQDVLYVDPSFCRAKEIHLRLGLMFKVNTDYKSSLKHFQLALIDCNPCTLSNAEIQFHIAH
  LYETQRKYHSAKEAYEQLLQTENLPAQVKATVLQQLGWMHHNMDLVGDKATKESYAIQYLQKSLEADPNS
  GQSWYFLGRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQ
  DAIKCYLNAARSKRCSNTSTLAARIKFLQNGSDNWNGGQSLSHHPVQQVYSLCLTPQKLQHLEQLRANRD
  NLNPAQKHQLEQLESQFVLMQQMRHKEVAQVRTTGIHNGAITDSSLPTNSVSNRQPHGALTRVSSVSQPG
  VRPACVEKLLSSGAFSAGCIPCGTSKILGSTDTILLGSNCIAGSESNGNVPYLQQNTHTLPHNHTDLNSS
  TEEPWRKQLSNSAQGLHKSQSSCLSGPNEEQPLFSTGSAQYHQATSTGIKKANEHLTLPSNSVPQGDADS
  HLSCHTATSGGQQGIMFTKESKPSKNRSLVPETSRHTGDTSNGCADVKGLSNHVHQLIADAVSSPNHGDS
  PNLLIADNPQLSALLIGKANGNVGTGTCDKVNNIHPAVHTKTDHSVASSPSSAISTATPSPKSTEQRSIN
  SVTSLNSPHSGLHTVNGEGLGKSQSSTKVDLPLASHRSTSQILPSMSVSICPSSTEVLKACRNPGKNGLS
  NSCILLDKCPPPRPPTSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPKNPVTVIRGLAGALKL
  DLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWRCESNRSHTTIAKYAQYQASSFQESLRE
  ENEKRTQHKDHSDNESTSSENSGRRRKGPFKTIKFGTNIDLSDNKKWKLQLHELTKLPAFARVVSAGNLL
  THVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEDYWGVLNDFCEKNNLNFL
  MSSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAVGWCNNIAWNVGPLTACQYKLAVERYEWN
  KLKSVKSPVPMVHLSWNMARNIKVSDPKLFEMIKYCLLKILKQYQTLREALVAAGKEVIWHGRTNDEPAH
  YCSICEVEVFNLLFVTNESNTQKTYIVHCHDCARKTSKSLENFVVLEQYKMEDLIQVYDQFTLALSLSSS
  S
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001258259
• ORF Size: 3993 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001258259.1, NP_001245188.1
• RefSeq Size: 6478 bp
• RefSeq ORF: 3996 bp
• Locus ID: 7404
• Cytogenetics: Yq11.221
• Protein Families: Transmembrane
• Gene Summary: 'This gene encodes a protein containing tetratricopeptide repeats which are thought to be involved in protein-protein interactions. The encoded protein is also a minor histocompatibility antigen which may induce graft rejection of male stem cell grafts. A large number of alternatively spliced transcripts have been observed for this gene, but the full length nature of some of these variants has not been determined. [provided by RefSeq, Apr 2012]'