BOULE (BOLL) (NM_001284362) Human Tagged ORF Clone
CAT#: RG237222
- TrueORF®
BOLL (GFP-tagged) - Human boule-like RNA-binding protein (BOLL), transcript variant 5
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Specifications
Product Data | |
Tag | TurboGFP |
Symbol | BOLL |
Synonyms | BOULE |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG237222 representing NM_001284362.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCAAACATCAAACCAGATGCAAACAGATTCATTATCTCCATCCCCTAATCCTGTGTCACCTGTGCCT TTGAATAACCCAACAAGTGCCCCAAGATATGGAACAGTGATCCCTAATCGCATCTTTGTAGGAGGAATT GATTTTAAGACAAACGAAAGTGATTTAAGAAAATTTTTTTCCCAGTATGGGTCTGTGAAAGAAGTGAAG ATTGTAAATGACAGAGCTGGAGTATCCAAAGGGTATGGTTTCGTCACTTTTGAAACACAAGAAGATGCA CAAAAAATTTTACAAGAGGCTGAAAAACTTAATTATAAGGATAAGAAGCTGAACATTGGTCCAGCAATA AGAAAACAACAAGTAGGGATCCCTCGTTCTAGTATAATGCCAGCAGCTGGAACAATGTATCTAACAACT TCAACTGGATATCCTTATACTTACCATAATGGTGTTGCTTATTTTCATACTCCAGAGGTAACTTCGGTC CCACCGCCTTGGCCTTCACGTTCTGTATGTAGCTCCCCTGTGATGGTAGCTCAGCCCATTTATCAGCAA CCTGCATATCACTACCAGGCCACCACACAGTATTTACCAGGACAGTGGCAGTGGAGTGTTCCTCAGCCT TCTGCCTCTTCTGCTCCATTCTTATACCTGCAACCTTCTGAGGTTATTTATCAACCAGTGGAAATTGCA CAGGATGGTGGATGTGTTCCTCCTCCACTGTCTCTGATGGAAACTTCAGTTCCAGAGCCTTATTCTGAT CATGGAGTTCAAGCAACATATCACCAGGTTTATGCTCCAAGTGCCATCACTATGCCTGCGCCTGTGATG CAGCCTGAGCCAATTAAAACAGTGTGGAGCATTCATTAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC >Peptide sequence encoded by RG237222
Blue=ORF Red=Cloning site Green=Tag(s) MQTSNQMQTDSLSPSPNPVSPVPLNNPTSAPRYGTVIPNRIFVGGIDFKTNESDLRKFFSQYGSVKEVK IVNDRAGVSKGYGFVTFETQEDAQKILQEAEKLNYKDKKLNIGPAIRKQQVGIPRSSIMPAAGTMYLTT STGYPYTYHNGVAYFHTPEVTSVPPPWPSRSVCSSPVMVAQPIYQQPAYHYQATTQYLPGQWQWSVPQP SASSAPFLYLQPSEVIYQPVEIAQDGGCVPPPLSLMETSVPEPYSDHGVQATYHQVYAPSAITMPAPVM QPEPIKTVWSIHY TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001284362 |
ORF Size | 867 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reference Data | |
RefSeq | NM_001284362.2 |
RefSeq Size | 2925 bp |
RefSeq ORF | 870 bp |
Locus ID | 66037 |
Cytogenetics | 2q33.1 |
MW | 32.4 kDa |
Gene Summary | This gene belongs to the DAZ gene family required for germ cell development. It encodes an RNA-binding protein which is more similar to Drosophila Boule than to human proteins encoded by genes DAZ (deleted in azoospermia) or DAZL (deleted in azoospermia-like). Loss of this gene function results in the absence of sperm in semen (azoospermia). Histological studies demonstrated that the primary defect is at the meiotic G2/M transition. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
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