Arp3 (ACTR3) (NM_001277140) Human Tagged ORF Clone

CAT#: RG237761

ACTR3 (GFP-tagged) - Human ARP3 actin-related protein 3 homolog (yeast) (ACTR3), transcript variant 2

Specifications

Product Data

• Tag: TurboGFP
• Symbol: ACTR3
• Synonyms: ARP3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG237761 representing NM_001277140.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAAAGGTGTTGATGACCTAGACTTCTTCATTGGTGATGAAGCAATAGAAAAACCTACATATGCAACA
  AAGTGGCCAATCCGCCATGGTATAGTTGAAGATTGGGACTTAATGGAAAGGTTTATGGAGCAAGTGATC
  TTTAAATATTTAAGGGCAGAACCTGAAGACCATTATTTTCTTTTGACTGAACCTCCATTGAATACTCCA
  GAAAACAGGGAATATACTGCTGAAATAATGTTTGAGTCCTTCAATGTTCCAGGCTTGTACATTGCTGTG
  CAGGCTGTTCTTGCCTTAGCTGCATCTTGGACCTCAAGACAAGTAGGAGAACGGACGTTGACCGGTACG
  GTAATAGACAGTGGAGATGGTGTCACTCATGTCATTCCTGTGGCTGAAGGGTATGTGATTGGCAGCTGT
  ATTAAACACATTCCAATCGCAGGACGAGATATAACATATTTTATTCAGCAACTGCTGAGAGACCGAGAA
  GTAGGAATCCCTCCAGAACAATCCTTGGAAACTGCTAAGGCAGTAAAGGAGCGCTATAGTTATGTCTGC
  CCAGATTTAGTAAAAGAATTTAACAAGTATGATACAGATGGGTCAAAATGGATTAAACAGTATACTGGA
  ATCAATGCTATCTCAAAGAAAGAGTTTTCTATCGATGTTGGTTATGAGAGATTTTTGGGACCTGAAATC
  TTTTTTCATCCAGAGTTTGCTAATCCAGACTTTACACAACCTATCTCAGAAGTTGTAGATGAAGTAATT
  CAGAATTGTCCTATTGATGTCAGACGTCCTCTCTACAAGAATATTGTCCTCTCTGGAGGTTCAACCATG
  TTCAGGGACTTTGGACGTCGCTTGCAAAGAGATTTGAAAAGAACTGTAGATGCCCGGCTGAAATTAAGT
  GAGGAATTGAGTGGTGGTAGATTGAAGCCAAAACCTATTGATGTACAAGTCATTACACACCACATGCAG
  CGATATGCAGTTTGGTTTGGAGGATCAATGCTGGCTTCCACGCCTGAGTTCTACCAAGTATGCCACACC
  AAAAAGGATTATGAAGAAATTGGACCTAGCATTTGTCGTCACAATCCAGTGTTTGGAGTCATGTCG
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG237761
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MKGVDDLDFFIGDEAIEKPTYATKWPIRHGIVEDWDLMERFMEQVIFKYLRAEPEDHYFLLTEPPLNTP
  ENREYTAEIMFESFNVPGLYIAVQAVLALAASWTSRQVGERTLTGTVIDSGDGVTHVIPVAEGYVIGSC
  IKHIPIAGRDITYFIQQLLRDREVGIPPEQSLETAKAVKERYSYVCPDLVKEFNKYDTDGSKWIKQYTG
  INAISKKEFSIDVGYERFLGPEIFFHPEFANPDFTQPISEVVDEVIQNCPIDVRRPLYKNIVLSGGSTM
  FRDFGRRLQRDLKRTVDARLKLSEELSGGRLKPKPIDVQVITHHMQRYAVWFGGSMLASTPEFYQVCHT
  KKDYEEIGPSICRHNPVFGVMS
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001277140
• ORF Size: 1101 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001277140.1, NP_001264069.1
• RefSeq Size: 5499 bp
• RefSeq ORF: 1104 bp
• Locus ID: 10096
• Cytogenetics: 2q14.1
• Protein Families: Druggable Genome
• MW: 42.5 kDa
• Gene Summary: The specific function of this gene has not yet been determined; however, the protein it encodes is known to be a major constituent of the ARP2/3 complex. This complex is located at the cell surface and is essential to cell shape and motility through lamellipodial actin assembly and protrusion. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Mar 2013]