Rabenosyn 5 (RBSN) (NM_001302378) Human Tagged ORF Clone

CAT#: RG239555

RBSN (GFP-tagged) - Human rabenosyn, RAB effector (RBSN), transcript variant 2

Specifications

Product Data

• Tag: TurboGFP
• Symbol: RBSN
• Synonyms: Rabenosyn-5; ZFYVE20
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239555 representing NM_001302378.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCTTCTCTGGACGACCCAGGGGAAGTGAGGGAGGGCTTCCTCTGCCCTCTGTGCCTGAAGGATCTG
  CAGTCTTTCTATCAGCTTCACTCACATTACGAGGAAGAACACTCAGGGGAAGACCGTGATGTCAAAGGG
  CAAATTAAAAGTCTTGTCCAGAAGGCTAAAAAAGCAAAGGACAGGTTGTTGAAACGAGAAGGGGATGAT
  CGAGCAGAGTCAGGGACCCAAGGATATGAGTCTTTCAGCTATGGAGGGGTTGATCCTTACATGTGGGAA
  CCCCAGGAGCTTGGTGCTGTGAGAAGCCATCTTTCCGACTTCAAAAAACACCGAGCTGCTAGAATTGAC
  CACTATGTTGTGGAAGTCAATAAACTAATAATCAGGTTAGAGAAGCTCACTGCATTTGACAGAACAAAT
  ACTGAGTCTGCAAAGATTCGAGCAATAGAAAAGTCTGTGGTGCCTTGGGTCAACGACCAGGATGTCCCT
  TTCTGTCCAGACTGTGGGAATAAGTTCAGCATCCGGAACCGCCGCCACCACTGCCGCCTCTGCGGGTCT
  ATTATGTGCAAGAAGTGTATGGAGCTCATCAGCCTTCCCTTGGCAAACAAGCTCACCAGTGCCAGCAAG
  GAGTCCCTGAGCACCCACACCAGCCCCAGCCAGTCACCCAACAGTGTCCATGGCTCCCGCCGAGGCAGC
  ATCAGCAGCATGAGCAGTGTCAGCTCGGTCCTGGATGAGAAGGACGATGACCGGATCCGCTGCTGTACA
  CACTGCAAGGACACGCTGCTCAAGAGAGAGCAGCAGATTGATGAGAAGGAGCACACACCTGACATCGTG
  AAGCTCTACGAGAAATTACGACTTTGCATGGAGAAAGTTGACCAGAAAGCTCCAGAATACATCAGGATG
  GCAGCATCATTAAATGCTGGGGAGACAACCTACAGTCTGGAACATGCCAGTGACCTTCGAGTGGAAGTG
  CAGAAAGTGTATGAGCTGATAGACGCTTTAAGTAAGAAGATCTTAACCTTGGGCTTGAACCAGGACCCT
  CCACCACATCCAAGCAATTTGCGGCTGCAGAGAATGATCAGATACTCAGCTACACTTTTTGTGCAGGAA
  AAGTTGCTTGGTTTGATGTCACTGCCAACCAAAGAACAGTTTGAGGAACTGAAAAAGAAAAGGAAGGAG
  GAAATGGAGAGGAAGAGGGCCGTGGAGAGACAAGCTGCCCTGGAGTCCCAGCGAAGGCTTGAGGAAAGG
  CAGAGTGGCCTGGCTTCTCGAGCGGCCAACGGGGAGGTGGCATCTCTCCGCAGGGGCCCTGCCCCCTTG
  AGAAAGGCTGAGGGCTGGCTCCCACTGTCAGGAGGTCAGGGGCAGAGTGAGGACTCAGACCCGCTCCTC
  CAGCAGATCCACAACATCACATCATTCATCAGGCAGGCCAAGGCCGCGGGCCGCATGGATGAAGTGCGC
  ACTCTGCAGGAGAACCTGCGGCAGCTGCAGGACGAGTATGACCAGCAGCAGACAGAGAAGGCCATCGAG
  CTGTCCCGGAGGCAGGCTGAGGAGGAGGACCTGCAGCGGGAACAGCTGCAGATGTTGCGTGAACGGGAG
  TTGGAACGAGAAAGGGAGCAGTTTCGGGTGGCATCCCTGCACACACGGACTCGGTCCCTGGACTTCAGA
  GAAATCGGCCCTTTTCAGCTGGAGCCCAGCAGAGAGCCTCGCACCCACCTTGCTTATGCTTTGGATCTA
  GGCTCTTCCCCAGTTCCAAGCAGCACAGCTCCCAAGACCCCTTCACTTAGCTCAACTCAACCCACCAGA
  GTGTGGTCTGGGCCCCCAGCCGTTGGCCAGGAGCGCTTACCCCAGAGCAGCATGCCACAGCAACATGAG
  GGGCCCTCCTTAAACCCCTTTGATGAGGAAGACCTCTCCAGCCCCATGGAAGAGGCCACTACTGGTCCT
  CCTGCTGCAGGGGTTTCCTTAGACCCTTCAGCCCGCATCCTGAAAGAGTACAATCCTTTCGAGGAAGAG
  GACGAGGAGGAGGAAGCAGTGGCAGGGAATCCATTCATTCAGCCAGACAGCCCAGCTCCTAACCCCTTC
  AGTGAGGAAGACGAACATCCCCAGCAGAGGCTCTCAAGCCCTCTGGTTCCTGGTAACCCCTTTGAGGAA
  CCCACCTGTATCAACCCCTTTGAGATGGACAGTGACAGTGGGCCAGAGGCTGAGGAGCCCATAGAGGAA
  GAGCTCCTCCTGCAGCAGATCGATAACATCAAGGCATACATCTTTGATGCCAAGCAGTGCGGCCGCCTG
  GATGAGGTAGAGGTGCTGACAGAGAATCTGCGGGAGCTGAAGCACACCCTGGCCAAGCAGAAGGGGGGC
  ACTGAC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239555
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MASLDDPGEVREGFLCPLCLKDLQSFYQLHSHYEEEHSGEDRDVKGQIKSLVQKAKKAKDRLLKREGDD
  RAESGTQGYESFSYGGVDPYMWEPQELGAVRSHLSDFKKHRAARIDHYVVEVNKLIIRLEKLTAFDRTN
  TESAKIRAIEKSVVPWVNDQDVPFCPDCGNKFSIRNRRHHCRLCGSIMCKKCMELISLPLANKLTSASK
  ESLSTHTSPSQSPNSVHGSRRGSISSMSSVSSVLDEKDDDRIRCCTHCKDTLLKREQQIDEKEHTPDIV
  KLYEKLRLCMEKVDQKAPEYIRMAASLNAGETTYSLEHASDLRVEVQKVYELIDALSKKILTLGLNQDP
  PPHPSNLRLQRMIRYSATLFVQEKLLGLMSLPTKEQFEELKKKRKEEMERKRAVERQAALESQRRLEER
  QSGLASRAANGEVASLRRGPAPLRKAEGWLPLSGGQGQSEDSDPLLQQIHNITSFIRQAKAAGRMDEVR
  TLQENLRQLQDEYDQQQTEKAIELSRRQAEEEDLQREQLQMLRERELEREREQFRVASLHTRTRSLDFR
  EIGPFQLEPSREPRTHLAYALDLGSSPVPSSTAPKTPSLSSTQPTRVWSGPPAVGQERLPQSSMPQQHE
  GPSLNPFDEEDLSSPMEEATTGPPAAGVSLDPSARILKEYNPFEEEDEEEEAVAGNPFIQPDSPAPNPF
  SEEDEHPQQRLSSPLVPGNPFEEPTCINPFEMDSDSGPEAEEPIEEELLLQQIDNIKAYIFDAKQCGRL
  DEVEVLTENLRELKHTLAKQKGGTD
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001302378
• ORF Size: 2352 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001302378.2
• RefSeq Size: 6540 bp
• RefSeq ORF: 2355 bp
• Locus ID: 64145
• Cytogenetics: 3p25.1
• Protein Families: Transcription Factors
• Protein Pathways: Endocytosis
• MW: 89.3 kDa
• Gene Summary: This gene encodes a protein that belongs to the FYVE zinc finger family of proteins. The encoded protein interacts with Ras-related proteins that regulate membrane trafficking. A missense mutation in this gene is associated with a defect in the early endocytic pathway. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]