Nit1 (NM_182668) Rat Untagged Clone

CAT#: RN200141

Nit1 (untagged ORF) - Rat nitrilase 1 (Nit1), transcript variant 1, (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Nit1
• Synonyms: MGC95151
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN200141 representing NM_182668
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGGCTTCATCACCAGGCCTCCTCATCAACTCCTGTCCCTTCTGTGTACCGGATACCGATTACCTC
  AAATCTCAGTACTTTGTACTCAGCCCAGGCCCAGAGCCATGGCAGAGTCCTCATCGACTTCCTGGGAGCT
  GCCCCTGGTGGCTGTGTGCCAGGTAACATCAACACCAAACAAGCAAGAGAACTTTAAAACGTGTGCTGAG
  CTGGTTCAAGAGGCTACCAGACTGGGCGCTTGCCTGGCCTTTCTGCCTGAGGCATTTGACTTTATTGCAC
  GAAATCCTGCCGAGACATTACTCCTGTCCGAACCACTGGATGGGGACCTTTTGGGACAATATAGCCAGCT
  TGCCAGGGAATGTGGAATCTGGCTGTCCTTGGGTGGTTTCCACGAGCGTGGCCAAGACTGGGAGCAGACT
  CAGAAAATCTACAATTGTCATGTGCTTCTGAACAGCAAGGGATCAGTAGTGGCCAGTTACAGGAAGACAC
  ATCTATGTGATGTAGAGATCCCAGGTCAGGGGCCTATGAGAGAAAGCAACTATACCATGCCTGGATACGC
  TCTTGAACCACCTGTCAAGACACCAGCAGGCAAGGTTGGTCTAGCAATCTGTTATGACATGCGGTTCCCT
  GAACTTTCTCTGAAATTGGCTCAAGCTGGGGCAGAAATACTTACTTATCCTTCAGCCTTTGGATCTGTTA
  CCGGCCCAGCCCACTGGGAGGTGCTGCTGCGGGCCCGCGCCATTGAGTCTCAGTGCTACGTGATAGCAGC
  AGCACAGTGTGGACGCCACCATGAGACAAGAGCAAGTTACGGCCACAGCATGGTGGTTGACCCCTGGGGC
  ACAGTGGTGGCCAGCTGTTCCGAGGGACCAGGCCTTTGCCTTGCTCGAATTGATCTCCACTTTCTGCAAC
  AGATGCGCCAACACTTGCCTGTGTTCCAGCATCGCAGACCTGACCTTTATGGCAGTCTCCTGCCACTCTC
  CTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_182668
• ORF Size: 984 bp
• Insert Size: 984
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_182668.2, NP_872609.2
• RefSeq Size: 1391
• RefSeq ORF: 984
• Locus ID: 289222
• Gene Summary: The protein encoded by this gene is a member of the nitrilase enzyme superfamily. Nitrilases are nonpeptidic C-N hydrolases. In mammals, nitrilases form small molecule C-N bonds such as acid amides, carbamates and ureas. Nit1-deficient mice display phenotypes including increased cell proliferation, resistance to DNA damage, and an increased number of N-nitrosomethylbenzylamine-induced murine forestomach tumors, suggesting a tumor suppressor function. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2015]
  Transcript Variant: This variant (1) encodes the longest isoform (a).