Nit1 (NM_001082580) Rat Untagged Clone

CAT#: RN200142

Nit1 (untagged ORF) - Rat nitrilase 1 (Nit1), transcript variant 2, (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Nit1
• Synonyms: MGC95151
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN200142 representing NM_001082580
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGAGTCCTCATCGACTTCCTGGGAGCTGCCCCTGGTGGCTGTGTGCCAGGTAACATCAACACCAA
  ACAAGCAAGAGAACTTTAAAACGTGTGCTGAGCTGGTTCAAGAGGCTACCAGACTGGGCGCTTGCCTGGC
  CTTTCTGCCTGAGGCATTTGACTTTATTGCACGAAATCCTGCCGAGACATTACTCCTGTCCGAACCACTG
  GATGGGGACCTTTTGGGACAATATAGCCAGCTTGCCAGGGAATGTGGAATCTGGCTGTCCTTGGGTGGTT
  TCCACGAGCGTGGCCAAGACTGGGAGCAGACTCAGAAAATCTACAATTGTCATGTGCTTCTGAACAGCAA
  GGGATCAGTAGTGGCCAGTTACAGGAAGACACATCTATGTGATGTAGAGATCCCAGGTCAGGGGCCTATG
  AGAGAAAGCAACTATACCATGCCTGGATACGCTCTTGAACCACCTGTCAAGACACCAGCAGGCAAGGTTG
  GTCTAGCAATCTGTTATGACATGCGGTTCCCTGAACTTTCTCTGAAATTGGCTCAAGCTGGGGCAGAAAT
  ACTTACTTATCCTTCAGCCTTTGGATCTGTTACCGGCCCAGCCCACTGGGAGGTGCTGCTGCGGGCCCGC
  GCCATTGAGTCTCAGTGCTACGTGATAGCAGCAGCACAGTGTGGACGCCACCATGAGACAAGAGCAAGTT
  ACGGCCACAGCATGGTGGTTGACCCCTGGGGCACAGTGGTGGCCAGCTGTTCCGAGGGACCAGGCCTTTG
  CCTTGCTCGAATTGATCTCCACTTTCTGCAACAGATGCGCCAACACTTGCCTGTGTTCCAGCATCGCAGA
  CCTGACCTTTATGGCAGTCTCCTGCCACTCTCCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001082580
• ORF Size: 876 bp
• Insert Size: 876
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_001082580.1, NP_001076049.1
• RefSeq Size: 1423
• RefSeq ORF: 876
• Locus ID: 289222
• Gene Summary: The protein encoded by this gene is a member of the nitrilase enzyme superfamily. Nitrilases are nonpeptidic C-N hydrolases. In mammals, nitrilases form small molecule C-N bonds such as acid amides, carbamates and ureas. Nit1-deficient mice display phenotypes including increased cell proliferation, resistance to DNA damage, and an increased number of N-nitrosomethylbenzylamine-induced murine forestomach tumors, suggesting a tumor suppressor function. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2015]
  Transcript Variant: This variant (2) differs in the 5' UTR and uses an alternate splice site in the 5' coding region, which results in initiation of translation from a downstream start codon compared to variant 1. It encodes isoform b, which has a shorter N-terminus compared to isoform a. Variants 2 and 4 encode the same isoform.