Pfkfb2 (NM_001033964) Rat Untagged Clone

CAT#: RN200494

Pfkfb2 (untagged ORF) - Rat 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 (Pfkfb2), transcript variant 2, (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Pfkfb2
• Synonyms: PFK-2; RH2K
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN200494 representing NM_001033964
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGAGAATAGTACATTTTCCACAGAAGACAGCAGCAGCAGCAGCTATAAACCCCACGCCTCAAACC
  TCCGAAGGGCAGGGAAGAAATGCTCATGGGCTTCCTACATGACCAACTCCCCAACACTCATTGTTATGAT
  TGGCTTGCCAGCCCGGGGTAAGACTTATGTGTCCAAGAAATTAACACGCTACCTGAACTGGATTGGAGTA
  CCTACCAAAGTGTTTAATCTTGGTGTATATCGACGGGAAGCAGTCAAGTCCTATAAGTCCTATGACTTCT
  TTCGACATGACAATGAAGAGGCCATGAAGATCCGCAAACAGTGTGCCCTGGTGGCACTGGAAGATGTGAA
  GGCCTACTTTACTGAAGAGAGTGGGCAGATCGCGGTGTTTGATGCCACCAATACCACTCGGGAGAGGAGG
  GACATGATTTTGAACTTTGCCAAGCAGAATGCCTTCAAGGTATTCTTTGTGGAATCTGTGTGTGATGATC
  CTGATGTCATTGCTGCCAATATTCTGGAGGTAAAAGTGTCAAGCCCTGACTACCCCGAAAGGAATAGGGA
  GAATGTGATGGAGGACTTCCTGAAGAGAATTGAGTGCTACAAGGTCACTTACCAGCCCCTTGACCCAGAC
  AACTATGATAAGGACCTCTCGTTCATAAAGGTGATGAATGTAGGCCAGAGGTTTCTGGTCAACAGAGTTC
  AGGACTACATCCAGAGTAAGATTGTCTACTACCTGATGAACATCCATGTCCATCCTCGCACCATCTATCT
  GTGCCGGCACGGAGAGAGCGAATTCAATCTTTTGGGAAAGATTGGGGGTGACTCTGGCCTTTCGTTGCGA
  GGAAAGCAGTTTGCTCAGGCTCTGAAGAAGTTTCTGGAGGAACAGGAGATCCAGGACCTCAAAGTGTGGA
  CAAGCCAGTTGAAGAGGACAATTCAGACTGCTGAGTCTCTTGGGGTGACCTATGAGCAATGGAAGATCCT
  AAATGAGATTGATGCTGGCGTGTGTGAGGAGATGACTTATTCGGAGATCGAACAACGGTATCCAGAGGAA
  TTTGCACTTCGAGATCAAGAGAAGTATCTGTATCGATATCCTGGTGGGGAGTCATACCAGGACCTGGTGC
  AGCGGCTGGAGCCTGTGATCATGGAGCTGGAGCGGCAAGGCAACGTCCTCGTTATCTCTCACCAGGCTGT
  CATGCGCTGCCTCCTGGCCTACTTCTTGGATAAAGGCGCAGATGAGTTGCCGTACTTGAGGTGCCCCCTC
  CACATCATCTTCAAACTTACTCCTGTGGCCTATGGTTGCAAAGTGGAAACAATTACACTGAATGTGGAAG
  CTGTGGACACACATCGTGACAAGCCAACTCACAACTTCCCCAAGAGCCAAACCCCTGTAAGGATGAGAAG
  GAACAGCTTTACGCCTCTGTCCAGTTCGAACACAATAAGGCGTCCAAGAAATTACAGTGTTGGGAGCCGA
  CCCCTCAAGCCCCTCAGCCCTCTCCGTGCCCTGGATATGCAAGAAGGGGCGGACCAGCCGAAGACCCAAG
  AAGTGGAGAATGTGCTGGCTAAGCATAGACGCCCCTCAATGGCATCCCTCACTTTGCTCTCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001033964
• Insert Size: 1605 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033964.2, NP_001029136.1
• RefSeq Size: 3755 bp
• RefSeq ORF: 1605 bp
• Locus ID: 24640
• Cytogenetics: 13q13
• Gene Summary: catalyzes the synthesis and degradation of fructose 2,6-bisphosphate [RGD, Feb 2006]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' end of the coding sequence compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.