Pfkfb2 (NM_001033964) Rat Tagged ORF Clone

CAT#: RR200494

Pfkfb2 (Myc-DDK-tagged ORF) - Rat 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 (Pfkfb2), transcript variant 2, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pfkfb2
• Synonyms: PFK-2; RH2K
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200494 representing NM_001033964
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGAGAATAGTACATTTTCCACAGAAGACAGCAGCAGCAGCAGCTATAAACCCCACGCCTCAAACC
  TCCGAAGGGCAGGGAAGAAATGCTCATGGGCTTCCTACATGACCAACTCCCCAACACTCATTGTTATGAT
  TGGCTTGCCAGCCCGGGGTAAGACTTATGTGTCCAAGAAATTAACACGCTACCTGAACTGGATTGGAGTA
  CCTACCAAAGTGTTTAATCTTGGTGTATATCGACGGGAAGCAGTCAAGTCCTATAAGTCCTATGACTTCT
  TTCGACATGACAATGAAGAGGCCATGAAGATCCGCAAACAGTGTGCCCTGGTGGCACTGGAAGATGTGAA
  GGCCTACTTTACTGAAGAGAGTGGGCAGATCGCGGTGTTTGATGCCACCAATACCACTCGGGAGAGGAGG
  GACATGATTTTGAACTTTGCCAAGCAGAATGCCTTCAAGGTATTCTTTGTGGAATCTGTGTGTGATGATC
  CTGATGTCATTGCTGCCAATATTCTGGAGGTAAAAGTGTCAAGCCCTGACTACCCCGAAAGGAATAGGGA
  GAATGTGATGGAGGACTTCCTGAAGAGAATTGAGTGCTACAAGGTCACTTACCAGCCCCTTGACCCAGAC
  AACTATGATAAGGACCTCTCGTTCATAAAGGTGATGAATGTAGGCCAGAGGTTTCTGGTCAACAGAGTTC
  AGGACTACATCCAGAGTAAGATTGTCTACTACCTGATGAACATCCATGTCCATCCTCGCACCATCTATCT
  GTGCCGGCACGGAGAGAGCGAATTCAATCTTTTGGGAAAGATTGGGGGTGACTCTGGCCTTTCGTTGCGA
  GGAAAGCAGTTTGCTCAGGCTCTGAAGAAGTTTCTGGAGGAACAGGAGATCCAGGACCTCAAAGTGTGGA
  CAAGCCAGTTGAAGAGGACAATTCAGACTGCTGAGTCTCTTGGGGTGACCTATGAGCAATGGAAGATCCT
  AAATGAGATTGATGCTGGCGTGTGTGAGGAGATGACTTATTCGGAGATCGAACAACGGTATCCAGAGGAA
  TTTGCACTTCGAGATCAAGAGAAGTATCTGTATCGATATCCTGGTGGGGAGTCATACCAGGACCTGGTGC
  AGCGGCTGGAGCCTGTGATCATGGAGCTGGAGCGGCAAGGCAACGTCCTCGTTATCTCTCACCAGGCTGT
  CATGCGCTGCCTCCTGGCCTACTTCTTGGATAAAGGCGCAGATGAGTTGCCGTACTTGAGGTGCCCCCTC
  CACATCATCTTCAAACTTACTCCTGTGGCCTATGGTTGCAAAGTGGAAACAATTACACTGAATGTGGAAG
  CTGTGGACACACATCGTGACAAGCCAACTCACAACTTCCCCAAGAGCCAAACCCCTGTAAGGATGAGAAG
  GAACAGCTTTACGCCTCTGTCCAGTTCGAACACAATAAGGCGTCCAAGAAATTACAGTGTTGGGAGCCGA
  CCCCTCAAGCCCCTCAGCCCTCTCCGTGCCCTGGATATGCAAGAAGGGGCGGACCAGCCGAAGACCCAAG
  AAGTGGAGAATGTGCTGGCTAAGCATAGACGCCCCTCAATGGCATCCCTCACTTTGCTCTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200494 representing NM_001033964
  Red=Cloning site Green=Tags(s)
  
  MSENSTFSTEDSSSSSYKPHASNLRRAGKKCSWASYMTNSPTLIVMIGLPARGKTYVSKKLTRYLNWIGV
  PTKVFNLGVYRREAVKSYKSYDFFRHDNEEAMKIRKQCALVALEDVKAYFTEESGQIAVFDATNTTRERR
  DMILNFAKQNAFKVFFVESVCDDPDVIAANILEVKVSSPDYPERNRENVMEDFLKRIECYKVTYQPLDPD
  NYDKDLSFIKVMNVGQRFLVNRVQDYIQSKIVYYLMNIHVHPRTIYLCRHGESEFNLLGKIGGDSGLSLR
  GKQFAQALKKFLEEQEIQDLKVWTSQLKRTIQTAESLGVTYEQWKILNEIDAGVCEEMTYSEIEQRYPEE
  FALRDQEKYLYRYPGGESYQDLVQRLEPVIMELERQGNVLVISHQAVMRCLLAYFLDKGADELPYLRCPL
  HIIFKLTPVAYGCKVETITLNVEAVDTHRDKPTHNFPKSQTPVRMRRNSFTPLSSSNTIRRPRNYSVGSR
  PLKPLSPLRALDMQEGADQPKTQEVENVLAKHRRPSMASLTLLS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001033964
• ORF Size: 1602 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033964.1, NM_001033964.2, NP_001029136.1
• RefSeq Size: 3755 bp
• RefSeq ORF: 1605 bp
• Locus ID: 24640
• Cytogenetics: 13q13
• MW: 61.7 kDa
• Gene Summary: catalyzes the synthesis and degradation of fructose 2,6-bisphosphate [RGD, Feb 2006]