Smpdl3b (NM_001025737) Rat Untagged Clone

CAT#: RN200955

Smpdl3b (untagged ORF) - Rat sphingomyelin phosphodiesterase, acid-like 3B (Smpdl3b), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Smpdl3b
• Synonyms: MGC116117
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN200955 representing NM_001025737
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGCTGCTCGGGTGGCTGATATTCCTGGCTTCCTGGGGAGTCGCAGGGGCTGAATTAGGGAGGTTCT
  GGCACATCTCCGACCTCCATCTGGACCCCAACTACACAGTATCCAAAGACCCCCTCCGGGTGTGCCCGTC
  AGCCGGCTCCCAGCCCGTGCTAAATGCAGGCCCCTGGGGTGACTACCTCTGCGATTCTCCTTGGGCCCTT
  ATCAACTCTTCCATCTATGCCATGAAGGAGATCGAACCAAAGCCAGACTTCATTTTCTGGACAGGGGATG
  ACACACCACACGTCCCCAATGAGAGGCTGGGGGAGGGAGCTGTGCTGTCGATGGTGGACCGTCTGACCAA
  CCTCATCAAGGAGGTCTTTCCAGGTACTAAAGTCTATGCTGCTCTGGGAAATCATGACTTTCACCCTAAG
  AACCAGCTCCCAGCCCAGAGCAACAGCATCTACACTCATGTGGCAGAGCTGTGGAGACCCTGGCTTAGCA
  ACGAATCCTTCACTCTCTTCAAAGAAGGTGCCTTCTATTCTGAGAAGTTGCCGAGTCCCAGCAAGACAGG
  GCGAGTTGTGGTCCTCAACACCAATCTGTACTACAGCAGCAATGAGCAGACAGCCGGCATGGCCGACCCA
  GGCCAGCAGTTCCAGTGGCTAGGAGATGTCCTAAGCAATGCATCTCGGAACGGGGAGATGGTGTACATTA
  TTGGCCACGTGCCCCCAGGGTTCTTTGAGAAGACACAGGACAAGGCCTGGTTCCGAGAGAGCTTCAACGA
  GGAGTATCTGAAGGTGGTTCAGCAGCACCATCGCGTCATAGCAGGGCAGTTCTTTGGGCACCACCATACC
  GACAGCTTCCGAATGTTCTACAGCAGCACAGGTGCCCCCATAAGCGTCATGTTCCTCACACCCGGGGTCA
  CCCCGTGGAAGACCACATTACCTGGAGTGGTCGATGGGGCCAACAATCCAGCGATCCGCATTTTTGAGTA
  TGACCGAGCCACTCTCAACTTGAAGGACATGGTGACTTACTACTTGAACCTGAGGCAGGCGGATACACAA
  GAGACCCCGCAGTGGGAGCAGGAATACCGCCTGACAGAGGCCTACGAGGTGCAGGACGCCAGCACCAGTT
  CTATGTATACAGCACTGACCCGCATCGCCAGTGAGCAGCACATCCTGCAACGTTATTACGTCTACAACTC
  GGTCAGCTACAGCCATCAGCCCTGTGAGGACGTCTGCCGAAGGGAGCATGTGTGCGCCATACAGCACGTG
  GAGTTCGACACTTACGCCACCTGCTTGCATGGTCTTGGTGCCAACCTGGTACCTGGTTTCCTGCTCATAC
  TGACTCTGCTGACGAGCCTACACGTATTGGAGGTGCTATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001025737
• Insert Size: 1371 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025737.1, NP_001020908.1
• RefSeq Size: 1923 bp
• RefSeq ORF: 1371 bp
• Locus ID: 362619
• Cytogenetics: 5q36