Jag1 (NM_019147) Rat Untagged Clone

CAT#: RN203938

Jag1 (untagged ORF) - Rat jagged 1 (Jag1), (10 ug)

Product Images

Circular map for RN203938

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Jag1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >NM_019147 ORF sequence, RN203938 may differ due to SNPs.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCGGTCCCCACGGACGCGCGGCCGGCCGGGGCGCCCCTTGAGCCTTCTGCTCGCCCTGCTCTGTGCC
  CTGCGAGCCAAGGTGTGCGGGGCCTCGGGTCAATTTGAGCTGGAGATCCTGTCCATGCAGAACGTGAAC
  GGAGAGTTACAGAATGGGAACTGTTGCGGCGGAGCCCGGAACCCTGGCGACCGCAAGTGCACCCGCGAC
  GAGTGTGACACGTACTTCAAAGTGTGCCTCAAGGAGTATCAGTCCCGCGTCACGGCCGGGGGACCCTGC
  AGCTTCGGCTCAGGGTCTACGCCTGTCATCGGGGGCAATACCTTCAATCTCAAGGCCAGCCGCGGCAAC
  GACCGTAATCGCATCGTACTGCCTTTCAGTTTCGCCTGGCCGAGGTCCTACACTTTGCTGGTGGAGGCA
  TGGGATTCCAGTAATGACACTATTCAACCTGATAGCATAATTGAAAAGGCTTCTCACTCAGGCATGATA
  AACCCCAGCCGGCAGTGGCAGACACTGAAACAGAACACAGGGATTGCCCACTTTGAGTATCAGATCCGA
  GTGACCTGTGATGACCACTACTATGGCTTTGGCTGCAACAAGTTCTGTCGGCCCAGAGATGACTTCTTT
  GGACATTATGCCTGCGACCAGAATGGCAACAAAACCTGCATGGAAGGCTGGATGGGTCCTGAGTGTAAC
  AAAGCAATATGCCGACAGGGCTGCAGTCCCAAGCATGGCTCCTGCAAACTCCCAGGTGACTGCAGGTGC
  CAGTATGGTTGGCAGGGCCTGTACTGTGATAAGTGCATCCCGCACCCAGGATGTGTCCACGGCACCTGC
  AATGAACCCTGGCAGTGCCTCTGTGAAACCAACTGGGGTGGACAGCTCTGTGACAAAGATCTGAATTAC
  TGTGGGACTCATCAGCCCTGTCTCAACCGGGGAACATGTAGCAACACTGGCCCTGACAAATACCAGTGT
  TCCTGCCCCGAGGGCTACTCAGGACCGAACTGTGAAATTGCTGAGCATGCCTGCCTCTCTGATCCCTGC
  CACAACAGAGGCAGCTGCAAGGAGACCTCCTCGGGCTTTGAGTGTGAGTGTTCTCCAGGCTGGACTGGA
  CCCACGTGTTCCACAAACATCGATGACTGTTCTCCAAATAACTGTTCCCATGGAGGCACCTGCCAGGAT
  CTAGTGAATGGATTCAAGTGCGTGTGCCCACCCCAGTGGACTGGCAAGACTTGTCAGTTAGATGCAAAT
  GAGTGCGAGGCCAAGCCTTGTGTAAATGCCAGATCCTGTAAGAATCTGATTGCCAGCTACTACTGTGAT
  TGTCTTCCTGGCTGGATGGGTCAGAATTGTGACATAAATATCAATGACTGCCTTGGCCAGTGTCAGAAT
  GATGCCTCCTGTCGGGATTTGGTTAATGGTTATCGCTGTATCTGTCCACCTGGCTATGCGGGCGATCAC
  TGTGAAAGAGACATCGATGAATGTGCTAGCAACCCCTGCTTGAATGGGGGTCACTGTCAGAATGAAATT
  AACAGATTCCAGTGTCTGTGTCCCACTGGTTTCTCTGGAAACCTCTGTCAGCTGGACATCGATTACTGC
  GAGCCCAACCCTTGCCAGAATGGCGCCCAATGCTACAATCGTGCCAGTGACTATTTCTGCAAGTGCCCT
  GAGGACTACGAGGGCAAGAACTGCTCACACCTGAAAGACCACTGCCGCACCACTCCCTGTGAAGTGATT
  GACAGCTGCACCGTGGCCATGGCCTCCAACGATACGCCTGAAGGGGTGCGGTATATCTCTTCCAACGTC
  TGTGGTCCCCATGGGAAGTGCAAGAGCGAGTCAGGAGGCAAATTCACCTGTGACTGTAACAAAGGCTTC
  ACCGGCACCTACTGCCATGAAAATATCAACGACTGTGAGGGCAACCCCTGTACGAACGGTGGCACGTGC
  ATCGACGGCGTCAACTCCTACAAGTGTATCTGTAGTGACGGCTGGGAGGGCGCGCACTGTGAGAACAAC
  ATAAATGACTGTAGCCAGAACCCTTGCCACTATGGGGGTACATGTCGAGACCTGGTCAATGACTTTTAC
  TGTGACTGCAAAAATGGGTGGAAAGGGAAAACCTGCCACTCGCGTGACAGCCAGTGTGACGAAGCCACG
  TGTAATAACGGTGGTACCTGCTATGATGAAGTGGATACGTTTAAGTGCATGTGTCCTGGTGGCTGGGAA
  GGAACAACCTGTAATATAGCTAGAAACAGTAGCTGCCTGCCGAACCCCTGTCATAATGGAGGCACCTGC
  GTGGTCAATGGAGACTCCTTCACCTGTGTCTGCAAAGAAGGCTGGGAGGGGCCGATTTGTACTCAAAAT
  ACCAACGACTGCAGTCCTCACCCTTGTTACAACAGCGGAACCTGTGTGGATGGAGATAACTGGTACCGG
  TGCGAATGTGCCCCTGGTTTTGCTGGGCCAGACTGCAGGATAAACATCAATGAGTGTCAGTCTTCCCCT
  TGTGCCTTTGGGGCCACCTGTGTGGATGAGATCAATGGCTACCAGTGTATCTGCCCTCCAGGACATAGT
  GGTGCCAAGTGCCATGAAGTTTCAGGGAGATCTTGCATCACCATGGGGAGAGTGATACTTGATGGGGCC
  AAGTGGGATGACGACTGTAACACCTGCCAGTGCCTGAATGGACGGGTGGCCTGCTCCAAGGTCTGGTGT
  GGCCCGAGACCTTGCCAGCTCCACAAAGGGCACGGCGAGTGTCCCAATGGGCAGAGCTGCATCCCAGTC
  CTGGATGACCAGTGTTTCGTGCGCCCCTGCACTGGTGCTGGCGAGTGTCGGTCCTCCAGCCTCCAGCCA
  GTGAAGACCAAGTGTACATCGGACTCTTATTACCAGGACAATTGTGCGAACATCACTTTCACCTTTAAC
  AAGGAGATGATGTCTCCAGGTCTCACCACTGAGCACATTTGCAGCGAATTGAGGAATCTGAATATTCTG
  AAGAATGTTTCTGCTGAGTATTCAATCTACATAGCCTGTGAGCCTTCCTTATCAGCAAACAATGAAATA
  CACGTGGCCATTTCTGCCGAAGACATCCGGGATGATGGAAACCCTGTCAAGGAAATTACTGATAAAATA
  ATAGATCTCGTTAGTAAACGGGATGGGAACAGCTCACTTATCGCTGCTGTTGCAGAAGTCAGAGTTCAG
  AGGCGCCCTCTGAAAAACAGAACAGATTTCCTGGTTCCTCTGCTGAGCTCTGTCTTAACAGTGGCTTGG
  GTCTGTTGCTTGGTGACAGCCTTCTACTGGTGTGTGCGGAAGCGGCGGCGGAAGCCCAGCAGCCACACC
  CACTCTGCCCCTGAGGACAACACCACCAACAATGTGCGCGAACAGCTGAACCAAATCAAAAACCCCATC
  GAGAAACACGGAGCCAACACAGTCCCCATCAAGGATTATGAGAACAAAAACTCCAAAATGTCAAAAATC
  AGGACACACAACTCGGAAGTGGAAGAGGATGATATGGATAAGCACCAGCAGAAAGTCCGGTTTGCCAAA
  CAGCCCGTGTACACGCTGGTAGACAGAGAGGAGAAGGCCCCCAGCGGCACGCCGACAAAACACCCGAAC
  TGGACAAACAAACAGGACAACAGAGACTTGGAAAGTGCCCAGAGCTTGAACCGGATGGAGTACATCGTA
  TAG
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
  Protein Sequence

  MRSPRTRGRPGRPLSLLLALLCALRAKVCGASGQFELEILSMQNVNGELQNGNCCGGARNPGDRKCTRD
  ECDTYFKVCLKEYQSRVTAGGPCSFGSGSTPVIGGNTFNLKASRGNDRNRIVLPFSFAWPRSYTLLVEA
  WDSSNDTIQPDSIIEKASHSGMINPSRQWQTLKQNTGIAHFEYQIRVTCDDHYYGFGCNKFCRPRDDFF
  GHYACDQNGNKTCMEGWMGPECNKAICRQGCSPKHGSCKLPGDCRCQYGWQGLYCDKCIPHPGCVHGTC
  NEPWQCLCETNWGGQLCDKDLNYCGTHQPCLNRGTCSNTGPDKYQCSCPEGYSGPNCEIAEHACLSDPC
  HNRGSCKETSSGFECECSPGWTGPTCSTNIDDCSPNNCSHGGTCQDLVNGFKCVCPPQWTGKTCQLDAN
  ECEAKPCVNARSCKNLIASYYCDCLPGWMGQNCDININDCLGQCQNDASCRDLVNGYRCICPPGYAGDH
  CERDIDECASNPCLNGGHCQNEINRFQCLCPTGFSGNLCQLDIDYCEPNPCQNGAQCYNRASDYFCKCP
  EDYEGKNCSHLKDHCRTTPCEVIDSCTVAMASNDTPEGVRYISSNVCGPHGKCKSESGGKFTCDCNKGF
  TGTYCHENINDCEGNPCTNGGTCIDGVNSYKCICSDGWEGAHCENNINDCSQNPCHYGGTCRDLVNDFY
  CDCKNGWKGKTCHSRDSQCDEATCNNGGTCYDEVDTFKCMCPGGWEGTTCNIARNSSCLPNPCHNGGTC
  VVNGDSFTCVCKEGWEGPICTQNTNDCSPHPCYNSGTCVDGDNWYRCECAPGFAGPDCRININECQSSP
  CAFGATCVDEINGYQCICPPGHSGAKCHEVSGRSCITMGRVILDGAKWDDDCNTCQCLNGRVACSKVWC
  GPRPCQLHKGHGECPNGQSCIPVLDDQCFVRPCTGAGECRSSSLQPVKTKCTSDSYYQDNCANITFTFN
  KEMMSPGLTTEHICSELRNLNILKNVSAEYSIYIACEPSLSANNEIHVAISAEDIRDDGNPVKEITDKI
  IDLVSKRDGNSSLIAAVAEVRVQRRPLKNRTDFLVPLLSSVLTVAWVCCLVTAFYWCVRKRRRKPSSHT
  HSAPEDNTTNNVREQLNQIKNPIEKHGANTVPIKDYENKNSKMSKIRTHNSEVEEDDMDKHQQKVRFAK
  QPVYTLVDREEKAPSGTPTKHPNWTNKQDNRDLESAQSLNRMEYIV*
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
  Fully Sequenced ORF

  >RN203938 representing NM_019147
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGTCCCCACGGACGCGCGGCCGGCCGGGGCGCCCCTTGAGCCTTCTGCTCGCCCTGCTCTGTGCCC
  TGCGAGCCAAGGTGTGCGGGGCCTCGGGTCAATTTGAGCTGGAGATCCTGTCCATGCAGAACGTGAACGG
  AGAGTTACAGAATGGGAACTGTTGCGGCGGAGCCCGGAACCCTGGCGACCGCAAGTGCACCCGCGACGAG
  TGTGACACGTACTTCAAAGTGTGCCTCAAGGAGTATCAGTCCCGCGTCACGGCCGGGGGACCCTGCAGCT
  TCGGCTCAGGGTCTACGCCTGTCATCGGGGGCAATACCTTCAATCTCAAGGCCAGCCGCGGCAACGACCG
  TAATCGCATCGTACTGCCTTTCAGTTTCGCCTGGCCGAGGTCCTACACTTTGCTGGTGGAGGCATGGGAT
  TCCAGTAATGACACTATTCAACCTGATAGCATAATTGAAAAGGCTTCTCACTCAGGCATGATAAACCCCA
  GCCGGCAGTGGCAGACACTGAAACAGAACACAGGGATTGCCCACTTTGAGTATCAGATCCGAGTGACCTG
  TGATGACCACTACTATGGCTTTGGCTGCAACAAGTTCTGTCGGCCCAGAGATGACTTCTTTGGACATTAT
  GCCTGCGACCAGAATGGCAACAAAACCTGCATGGAAGGCTGGATGGGTCCTGAGTGTAACAAAGCAATAT
  GCCGACAGGGCTGCAGTCCCAAGCATGGCTCCTGCAAACTCCCAGGTGACTGCAGGTGCCAGTATGGTTG
  GCAGGGCCTGTACTGTGATAAGTGCATCCCGCACCCAGGATGTGTCCACGGCACCTGCAATGAACCCTGG
  CAGTGCCTCTGTGAAACCAACTGGGGTGGACAGCTCTGTGACAAAGATCTGAATTACTGTGGGACTCATC
  AGCCCTGTCTCAACCGGGGAACATGTAGCAACACTGGCCCTGACAAATACCAGTGTTCCTGCCCCGAGGG
  CTACTCAGGACCGAACTGTGAAATTGCTGAGCATGCCTGCCTCTCTGATCCCTGCCACAACAGAGGCAGC
  TGCAAGGAGACCTCCTCGGGCTTTGAGTGTGAGTGTTCTCCAGGCTGGACTGGACCCACGTGTTCCACAA
  ACATCGATGACTGTTCTCCAAATAACTGTTCCCATGGAGGCACCTGCCAGGATCTAGTGAATGGATTCAA
  GTGCGTGTGCCCACCCCAGTGGACTGGCAAGACTTGTCAGTTAGATGCAAATGAGTGCGAGGCCAAGCCT
  TGTGTAAATGCCAGATCCTGTAAGAATCTGATTGCCAGCTACTACTGTGATTGTCTTCCTGGCTGGATGG
  GTCAGAATTGTGACATAAATATCAATGACTGCCTTGGCCAGTGTCAGAATGATGCCTCCTGTCGGGATTT
  GGTTAATGGTTATCGCTGTATCTGTCCACCTGGCTATGCGGGCGATCACTGTGAAAGAGACATCGATGAA
  TGTGCTAGCAACCCCTGCTTGAATGGGGGTCACTGTCAGAATGAAATTAACAGATTCCAGTGTCTGTGTC
  CCACTGGTTTCTCTGGAAACCTCTGTCAGCTGGACATCGATTACTGCGAGCCCAACCCTTGCCAGAATGG
  CGCCCAATGCTACAATCGTGCCAGTGACTATTTCTGCAAGTGCCCTGAGGACTACGAGGGCAAGAACTGC
  TCACACCTGAAAGACCACTGCCGCACCACTCCCTGTGAAGTGATTGACAGCTGCACCGTGGCCATGGCCT
  CCAACGATACTCCTGAAGGGGTGCGGTATATCTCTTCCAACGTCTGTGGTCCCCATGGGAAGTGCAAGAG
  CGAGTCAGGAGGCAAATTCACCTGTGACTGTAACAAAGGCTTCACCGGCACCTACTGCCATGAAAATATC
  AACGACTGTGAGGGCAACCCCTGTACGAACGGTGGCACGTGCATCGACGGCGTTAACTCCTACAAGTGTA
  TCTGTAGTGACGGCTGGGAGGGCGCGCACTGTGAGAACAACATAAATGACTGTAGCCAGAACCCTTGCCA
  CTATGGGGGTACATGTCGAGACCTGGTCAATGACTTTTACTGTGACTGCAAAAATGGGTGGAAAGGGAAA
  ACCTGCCACTCGCGTGACAGCCAGTGTGACGAAGCCACGTGTAATAACGGTGGTACCTGCTATGATGAAG
  TGGATACGTTTAAGTGCATGTGTCCTGGTGGCTGGGAAGGAACAACCTGTAATATAGCTAGAAACAGTAG
  CTGCCTGCCGAACCCCTGTCATAATGGAGGCACCTGCGTGGTCAATGGAGACTCCTTCACCTGTGTCTGC
  AAAGAAGGCTGGGAGGGGCCGATTTGTACTCAAAATACCAACGACTGCAGTCCTCACCCTTGTTACAACA
  GCGGAACCTGTGTGGATGGAGATAACTGGTACCGGTGCGAATGTGCCCCTGGTTTTGCTGGGCCAGACTG
  CAGGATAAACATCAATGAGTGTCAGTCTTCCCCTTGTGCCTTTGGGGCCACCTGTGTGGATGAGATCAAT
  GGCTACCAGTGTATCTGCCCTCCAGGACATAGTGGTGCCAAGTGCCATGAAGTTTCAGGGAGATCTTGCA
  TCACCATGGGGAGAGTGATACTTGATGGGGCCAAGTGGGATGACGACTGTAACACCTGCCAGTGCCTGAA
  TGGACGGGTGGCCTGCTCCAAGGTCTGGTGTGGCCCGAGACCTTGCCGGCTCCACAAAGGGCACGGCGAG
  TGTCCCAATGGGCAGAGCTGCATCCCAGTCCTGGATGACCAGTGTTTCGTGCGCCCCTGCACTGGTGCTG
  GCGAGTGTCGGTCCTCCAGCCTCCAGCCAGTGAAGACCAAGTGTACATCGGACTCTTATTACCAGGACAA
  TTGTGCGAACATCACTTTCACCTTTAACAAGGAGATGATGTCTCCAGGTCTCACCACTGAGCACATTTGC
  AGCGAATTGAGGAATCTGAATATTCTGAAGAATGTTTCTGCTGAGTATTCAATCTACATAGCCTGTGAGC
  CTTCCTTATCAGCAAACAATGAAATACACGTGGCCATTTCTGCCGAAGACATCCGGGATGATGGAAACCC
  TGTCAAGGAAATTACTGATAAAATAATAGATCTCGTTAGTAAACGGGATGGGAACAGCTCACTTATCGCT
  GCTGTTGCAGAAGTCAGAGTTCAGAGGCGCCCTCTGAAAAACAGAACAGATTTCCTGGTTCCTCTGCTGA
  GCTCTGTCTTAACAGTGGCTTGGGTCTGTTGCTTGGTGACAGCCTTCTACTGGTGTGTGCGGAAGCGGCG
  GCGGAAGCCCAGCAGCCACACCCACTCTGCCCCTGAGGACAACACCACCAACAATGTGCGCGAACAGCTG
  AACCAAATCAAAAACCCCATCGAGAAACACGGAGCCAACACAGTCCCCATCAAGGATTATGAGAACAAAA
  ACTCCAAAATGTCAAAAATCAGGACACACAACTCGGAAGTGGAAGAGGATGATATGGATAAGCACCAGCA
  GAAAGTCCGGTTTGCCAAACAGCCCGTGTACACGCTGGTAGACAGAGAGGAGAAGGTCCCCCAGCGCACG
  CCGACAAAACACCCGAACTGGACAAACAAACAGGACAACAGAGACTTGGAAAGTGCCCAGAGCTTGAACC
  GGATGGAGTACATCGTATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI Plasmid Map
• ACCN: NM_019147
• Insert Size: 3660 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019147.3
• RefSeq Size: 5600 bp
• RefSeq ORF: 3660 bp
• Locus ID: 29146
• UniProt ID: Q63722
• Cytogenetics: 3q36
• MW: 134.1 kDa
• Gene Summary: ligand responsible for activating Notch1 [RGD, Feb 2006]