Top1mt (NM_001002798) Rat Untagged Clone

CAT#: RN204378

Top1mt (untagged ORF) - Rat topoisomerase (DNA) I, mitochondrial (Top1mt), nuclear gene encoding mitochondrial protein, (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Top1mt
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN204378 representing NM_001002798
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCTGCTATGGCTCAGAGCTCTGTGCCGGCGTTTCCAGCATGTCCCCCGCCGCGTCCCCAGCCGCC
  AAGTGTCCCGGGGCTCGAAGGCGAGCAGAGCCGGGTGGGGAGAGACGAGCAAGAGCAGTGTGAAGTGGAA
  GCAACTGGAGCACAAGGGTCCGTGCTTCGCCCCAGCCTATGAGCCCCTTCCTGATGGAGTACGTTTCTTC
  TATGATGGCAAGCCAGTGAGGCTGAGCTTGGCAGCGGAGGAGGTTGCTACTTTCTATGGGAAAATGCTGC
  ATCTCGAGTGTACGACCAAGGAAGTCTTCCGGAGGAACTTCTTCAGCGACTGGCAGAAGGAAATGACAGC
  TGAGGAGAGGAAGCTCATCACACACCTGGACAAGTGCGACTTCTCGGAGATCCATAGGCACTTCATGGAA
  AGAGCTGAGGCCCGCAGGACCCTTCCCAGGGAGCAGAAGCAGAAACTAAAAGAAGAGGCAGAGAAGCTTC
  AGCAGGAGTTTGGCTACTGTATTTTAGATGGCCACAGAGAGAAAATAGGCAATTTCAAGACGGAACCACC
  CGGCTTGTTCCGCGGCCGAGGCGACCATCCCAAGATGGGGATGTTGAAGAGGAGGGTCATGCCAGAGGAT
  GTGGTCATTAACTGTAGCAGAGATTCCAAGATCCCAGAGCCCCCGGCTGGTCATCAGTGGAAAGAGGTCC
  GCTCGGATAACACAGTCATGTGGCTGGCTGCCTGGGTGGAGAACATCCAGAACTCCTTCAAGTACATCAT
  ACTGAACCCCAGCTCCAAGCCGAAGGGAGAGATGGATTGGCAGAAGTATGAGGTAGCACGACGCTTGAAG
  GGGGTTGTGGACAAGATCCGTGCTCAGTATCAGGCTGACTGGAAGTCACCAGAAATGAAGAAAAGGCAGC
  TGGCTGTAGCCCTTTATTTTATTGATAAGCTGGCACTGCGAACAGGAAATGAGAAGGAAGAGGGTGAGAC
  GGCCGACACCGTGGGCTGCTGTTCGCTCCGAGTGGAGCACGTCCGACTGCACACGCCCGCAGATGGCCAG
  GAGCATGTGGTAGAGCTGGACTTCTTGGGGAAGGATTCCATCCGTTATAAGAATCACGTGACGGTGGAGA
  AGCTTGTGTTCCAGAACCTTCAGCACTTCATGGAAGACAAGGACCCTAGGGACGACCTCTTCGATGCACT
  GACTACTTCCAGCCTGAACAAGCACTTGCAGGACTTGATGGAGGGGCTGACAGCCAAGGTGTTTCGGACC
  TACAATGCCTCCATCACTCTGCAGGAGCAGCTGCGGGTGCTGACCCGTGCTGAAGACAGCCTAACCTGCA
  AAGTCTTAGCTTACAACCGGGCAAACCGGGCTGTGGCGGTCCTCTGTAACCATCAGAGAGCAATTCCTAA
  GACCTTCGAGGAGTCCATGCAGACACTGCAGAAAAAGATTGAGACAAAGAAGGCACAGGTGGCTGAGGCA
  CAGGTGGAGCTGCAGAAGGCAGAGACTGACCTTAGAATGAGAGGGGACAGCAAGTCCAAAAGTTTCCTTC
  AGAAGCAGCAGCGGCTGCTGAAGCTGGAGGAGCAGCTGGCTCGACTGTGCACGAAGGCCACAGACAAGGA
  GGAGAATAAGCAAGTGGCCCTGGGCACCGCCAAGCTCAACTACCTGGACCCCCGGATCAGCATTGCATGG
  TGTAAGAGATTTGGTGTGCCCGTAGAGAAGATCTACAACAAGACCCAGAGAGAGAGGTTTGCTTGGGCCT
  TCAACCAGGCAGGTGAAGACTTTGAATTTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001002798
• Insert Size: 1782 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001002798.1, NP_001002798.1
• RefSeq Size: 1887 bp
• RefSeq ORF: 1782 bp
• Locus ID: 300029
• Cytogenetics: 7q34