Runx1 (NM_017325) Rat Untagged Clone

CAT#: RN206224

Runx1 (untagged ORF) - Rat runt related transcription factor 1 (Runx1), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Runx1
• Synonyms: Aml1; B; CBF-alpha-2; Cbfa2; PEA2-alpha; PEBP2-alpha
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RN206224 representing NM_017325.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCGTATCCCCGTAGATGCCAGCACGAGCCGCCGCTTCACGCCGCCTTCCACCGCGCTGAGCCCCGGC
  AAGATGAGCGAGGCGCTGCCGCTGGGCGCCCCGGATGGCGGCGCCGCCCTGGCCAGCAAGCTGAGGAGC
  GGCGACCGCAGCATGGTGGAGGTACTAGCTGACCACCCCGGCGAGCTAGTCCGCACCGACAGCCCCAAC
  TTCCTCTGCTCCGTGCTGCCCACTCACTGGCGCTGCAACAAGACCCTGCCCATCGCTTTCAAGGTGGTA
  GCGCTGGGCGACGTTCCGGATGGCACTCTGGTCACCGTCATGGCGGGCAATGACGAAAACTACTCGGCG
  GAGCTGAGAAACGCCACCGCGGCCATGAAGAACCAAGTGGCGAGATTCAACGACCTCAGGTTTGTCGGT
  CGGAGCGGCAGAGGCAAGAGCTTCACTCTGACCATCACCGTCTTTACAAACCCCCCACAAGTTGCCACC
  TACCATAGAGCCATCAAAATCACAGTGGACGGCCCCCGAGAACCCCGAAGACATCGGCAGAAACTAGAT
  GATCAGACCAAGCCCGGGAGTTTGTCCTTTTCCGAGCGGCTCAGTGAACTGGAGCAGCTGCGGCGCACG
  GCCATGAGGGTCAGCCCGCATCACCCAGCCCCCACGCCCAACCCTCGGGCCTCCTTGAACCACTCCACT
  GCCTTTAACCCTCAGCCTCAAAGTCAGATGCAGGATGCCAGGCAGATCCAGCCGTCCCCACCGTGGTCC
  TATGACCAGTCTTACCAGTACCTGGGGTCCATCACCTCTTCTGTGCACCCGGCGACACCCATTTCTCCC
  GGCCGAGCCAGTGGCATGACCAGCCTCTCAGCGGAACTTTCCAGTCGACTCTCAACAGCCCCGGACCTG
  ACAGCCTTCGGCGACCCGCGCCAGTTCCCCACGCTGCCGTCCATCTCCGACCCGCGCATGCACTACCCA
  GGCGCCTTCACCTACTCGCCTCCTGTCACATCGGGCATCGGCATTGGCATGTCAGCCATGAGCTCGACC
  TCTCGCTACCACACCTACCTGCCGCCGCCCTACCCAGGCTCCTCGCAGGCGCAGGCCGGGCCCTTCCAG
  ACCGGCTCGCCCTCCTACCACCTGTACTACGGCACCTCGGCAGGCTCCTACCAATTCTCCATGGTGGGC
  GGCGAGCGATCACCCCCGCGCATCCTGCCGCCCTGCACCAACGCGTCCACCGGAGCCGCGCTGCTCAAC
  CCCAGCCTCCCCAGCCAGAGCGACGTGGTGGAAACCGAGGGCAGCCATAGCAACTCGCCCACCAACATG
  CCACCCGCGCGCCTGGAGGAGGCTGTCTGGCGGCCCTACTGA
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT
  ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
  
  Protein Sequence

  MRIPVDASTSRRFTPPSTALSPGKMSEALPLGAPDGGAALASKLRSGDRSMVEVLADHPGELVRTDSPN
  FLCSVLPTHWRCNKTLPIAFKVVALGDVPDGTLVTVMAGNDENYSAELRNATAAMKNQVARFNDLRFVG
  RSGRGKSFTLTITVFTNPPQVATYHRAIKITVDGPREPRRHRQKLDDQTKPGSLSFSERLSELEQLRRT
  AMRVSPHHPAPTPNPRASLNHSTAFNPQPQSQMQDARQIQPSPPWSYDQSYQYLGSITSSVHPATPISP
  GRASGMTSLSAELSSRLSTAPDLTAFGDPRQFPTLPSISDPRMHYPGAFTYSPPVTSGIGIGMSAMSST
  SRYHTYLPPPYPGSSQAQAGPFQTGSPSYHLYYGTSAGSYQFSMVGGERSPPRILPPCTNASTGAALLN
  PSLPSQSDVVETEGSHSNSPTNMPPARLEEAVWRPY*
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
• Restriction Sites: SgfI-RsrII Plasmid Map
• ACCN: NM_017325
• Insert Size: 1353 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017325.2
• RefSeq Size: 2135 bp
• RefSeq ORF: 1353 bp
• Locus ID: 50662
• UniProt ID: Q63046
• Cytogenetics: 11q11
• MW: 48.6 kDa
• Gene Summary: putative transcription factor that may be involved in skeletal muscle gene regulation; human homolog is associated with a form of acute myeloid leukemia [RGD, Feb 2006]