Eif4g2 (NM_001017374) Rat Untagged Clone

CAT#: RN209576

Eif4g2 (untagged ORF) - Rat eukaryotic translation initiation factor 4, gamma 2 (Eif4g2), (10 ug)


  "NM_001017374" in other vectors (3)

Reconstitution Protocol

USD 1,230.00

6 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Symbol Eif4g2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN209576 representing NM_001017374
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

GTGGAGAGTGCGATTGCAGAAGGGGGTGCTTCTCGTTTCAGTGCTTCTTCGGGCGGAGGAGGAAGTAGGG
GTGCACCTCAGCACTATCCCAAGACTGCTGGCAACAGCGAGTTCCTGGGGAAAACCCCAGGGCAAAACGC
TCAGAAATGGATTCCTGCACGAAGCACTAGACGAGATGACAACTCCGCAGCAAACAACTCCGCAAATGAA
AAAGAACGACATGATGCAATCTTCAGGAAAGTAAGAGGCATACTAAATAAGCTTACTCCTGAAAAGTTTG
ACAAGCTATGCCTTGAGCTCCTCAATGTGGGTGTAGAGTCTAAACTCATCCTTAAAGGGGTCATACTGCT
GATTGTGGACAAAGCCCTAGAAGAGCCAAAGTATAGCTCACTGTATGCTCAGCTATGTCTGCGATTGGCA
GAAGATGCACCAAACTTTGATGGCCCAGCAGCAGAGGGTCAACCAGGACAGAAGCAAAGCACAACATTCA
GACGCCTCTTGATTTCCAAATTGCAAGATGAATTTGAAAACCGAACCAGAAATGTTGATGTCTATGATAA
GCGTGAAAATCCCCTCCTCCCTGAGGAGGAGGAACAGAGAGCCATTGCTAAGATCAAGATGTTGGGGAAC
ATCAAATTCATTGGAGAACTTGGCAAGCTTGATCTTATTCATGAATCTATCCTTCATAAGTGCATCAAAA
CACTTTTGGAAAAGAAGAAGAGAGTCCAACTCAAAGATATGGGAGAGGATTTGGAGTGCCTCTGTCAGAT
AATGAGGACAGTGGGACCTCGATTAGACCATGAACGAGCCAAGTCCTTAATGGATCAGTACTTTGCCAGA
ATGTGTTCCTTAATGTTAAGTAAGGAATTGCCAGCCAGGATTCGTTTCCTACTGCAGGATACCGTAGAGT
TGCGAGAGCACCATTGGGTTCCTCGCAAGGCTTTTCTTGACAATGGACCAAAGACGATCAATCAAATCCG
TCAAGATGCAGTAAAAGATCTAGGAGTGTTTATTCCTGCTCCTATGGCTCAAGGGAGGAATGACTTCTTC
CTGGAGGGACCGTTCATGCCGCCAAGGATGAAAATGGATAGGGACCCACTTGGGGGACTCGCTGATATGT
TTGGACAAATGCCAGGTAGTGGAATTGGTACTGGTCCAGGAGTTATCCAGGATAGATTTTCACCCACAAT
GGGACGTCATCGTTCAAATCAGCTCTTCAATGGCCATGGGGGACACATCATGCCTCCCACGCAATCGCAG
TTTGGAGAGATGGGGGGCAAGTTTATGAAAAGCCAGGGGCTAAGCCAGCTCTACCATAACCAGAGTCAGG
GACTCTTATCCCAGCTGCAAGGACAGTCGAAGGATATGCCACCTCGGTTTTCTAAGAAAGGACAGCTTAA
TGCAGATGAGATTAGTTTGAGGCCTGCTCAGTCGTTTCTAATGAATAAAAATCAGGTGCCAAAGCTTCAG
CCCCAGATAACTATGATTCCTCCCAGTGCACAGCCACCACGCACTCAAACACCACCTCTGGGACAGACAC
CTCAACTTGGTCTCAAAACTAATCCACCACTTATTCAGGAAAAGCCTGCCAAGACTAGCAAAAAGCCACC
ACCATCAAAGGAAGAACTACTTAAACTGACCGAAGCTGTTGTAACTGAGTATCTGAACAGTGGAAATGCC
AACGACGCTGTCAGTGGTGTGAGAGAAATGAGAGCTCCAAAACACTTTCTTCCTGAGATGTTAAGCAAAG
TGATCATCCTGTCACTTGATAGAAGTGATGAAGATAAAGAAAAAGCAAGCTCTTTGATCAGTTTACTCAA
ACAGGAAGGGATAGCCACAAGTGACAACTTTATGCAGGCTTTCCTGAATGTATTGGAGCAGTGCCCCAAA
CTGGAGGTTGACATCCCCTTGGTGAAATCTTACTTGGCACAGTTTGCGGCTCGTGCTATAATTTCAGAGT
TGGTGAGCATTTCAGAACTAGCTCAACCACTGGAGAGTGGCACCCACTTCCCTCTCTTCTTACTTTGTCT
TCAACAATTAGCTAAATTACAAGACCGAGAATGGTTAACCGAACTTTTTCAACAAAGCAAGGTCAATATG
CAGAAAATGCTGCCAGAAATTGATCAGAATAAGGATCGAATGTTGGAGATTTTGGAAGGAAAGGGACTGA
GTTTCTTATTCCCACTCCTTAAATTGGAGAAGGAACTATTGAAGCAAATTAAGCTGGATCCATCCCCTCA
AACTATATATAAATGGATTAAAGATAACATCTCTCCCAAACTTCATGTAGATAAAGGATTTGTGAACATC
TTAATGACCAGCTTCTTACAGTACATTTCTAGTGAAGTTAGCCCACCCAGCGATGAAACAGATTCTTCCT
CTGCTCCTTCCAAAGAACAGTTAGAGCAGGAAAAACAGCTGCTGCTCTCTTTTAAGCCAGTGATGCAGAA
GTTTCTTCATGATCATGTGGATCTACAGGTCAGTGCCCTGTATGCTTTGCAGGTGCACTGTTACAACAGC
AGCTTCCCAAAAGGCATGTTACTTCGATTTTTTGTTCATTTCTATGACATGGAAATTATTGAAGAGGAAG
CTTTCTTAGCTTGGAAGGAAGACATAACTCAAGAGTTTCCAGGAAAAGGCAAGGCTTTGTTCCAGGTGAA
TCAGTGGCTAACCTGGCTAGAAACTGCTGAAGAAGAAGAATCAGAGGAAGAAGCTGACTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001017374
Insert Size 2721 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001017374.2, NP_001017374.2
RefSeq Size 5008 bp
RefSeq ORF 2721 bp
Locus ID 361628
Cytogenetics 1q33
Gene Summary Translation initiation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F (eIF4F), which is a cap binding protein complex that consists of three subunits: eIF4A, eIF4E and eIF4G. The protein encoded by this gene shares similarity with the C-terminal region of eIF4G that contains the binding sites for eIF4A and eIF3; eIF4G, in addition, contains a binding site for eIF4E at the N-terminus. Unlike eIF4G, which supports cap-dependent and independent translation, this gene product functions as a general repressor of translation by forming translationally inactive complexes. In vitro and in vivo studies in human indicate that translation of this mRNA initiates exclusively at a non-AUG (GUG) codon. This also appears to be true for mouse and rat. [provided by RefSeq, Jul 2008]

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.