Eif4g2 (NM_001017374) Rat Tagged ORF Clone

CAT#: RR209576

Eif4g2 (Myc-DDK-tagged ORF) - Rat eukaryotic translation initiation factor 4, gamma 2 (Eif4g2), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Eif4g2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR209576 representing NM_001017374
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  GTGGAGAGTGCGATTGCAGAAGGGGGTGCTTCTCGTTTCAGTGCTTCTTCGGGCGGAGGAGGAAGTAGGG
  GTGCACCTCAGCACTATCCCAAGACTGCTGGCAACAGCGAGTTCCTGGGGAAAACCCCAGGGCAAAACGC
  TCAGAAATGGATTCCTGCACGAAGCACTAGACGAGATGACAACTCCGCAGCAAACAACTCCGCAAATGAA
  AAAGAACGACATGATGCAATCTTCAGGAAAGTAAGAGGCATACTAAATAAGCTTACTCCTGAAAAGTTTG
  ACAAGCTATGCCTTGAGCTCCTCAATGTGGGTGTAGAGTCTAAACTCATCCTTAAAGGGGTCATACTGCT
  GATTGTGGACAAAGCCCTAGAAGAGCCAAAGTATAGCTCACTGTATGCTCAGCTATGTCTGCGATTGGCA
  GAAGATGCACCAAACTTTGATGGCCCAGCAGCAGAGGGTCAACCAGGACAGAAGCAAAGCACAACATTCA
  GACGCCTCTTGATTTCCAAATTGCAAGATGAATTTGAAAACCGAACCAGAAATGTTGATGTCTATGATAA
  GCGTGAAAATCCCCTCCTCCCTGAGGAGGAGGAACAGAGAGCCATTGCTAAGATCAAGATGTTGGGGAAC
  ATCAAATTCATTGGAGAACTTGGCAAGCTTGATCTTATTCATGAATCTATCCTTCATAAGTGCATCAAAA
  CACTTTTGGAAAAGAAGAAGAGAGTCCAACTCAAAGATATGGGAGAGGATTTGGAGTGCCTCTGTCAGAT
  AATGAGGACAGTGGGACCTCGATTAGACCATGAACGAGCCAAGTCCTTAATGGATCAGTACTTTGCCAGA
  ATGTGTTCCTTAATGTTAAGTAAGGAATTGCCAGCCAGGATTCGTTTCCTACTGCAGGATACCGTAGAGT
  TGCGAGAGCACCATTGGGTTCCTCGCAAGGCTTTTCTTGACAATGGACCAAAGACGATCAATCAAATCCG
  TCAAGATGCAGTAAAAGATCTAGGAGTGTTTATTCCTGCTCCTATGGCTCAAGGGAGGAATGACTTCTTC
  CTGGAGGGACCGTTCATGCCGCCAAGGATGAAAATGGATAGGGACCCACTTGGGGGACTCGCTGATATGT
  TTGGACAAATGCCAGGTAGTGGAATTGGTACTGGTCCAGGAGTTATCCAGGATAGATTTTCACCCACAAT
  GGGACGTCATCGTTCAAATCAGCTCTTCAATGGCCATGGGGGACACATCATGCCTCCCACGCAATCGCAG
  TTTGGAGAGATGGGGGGCAAGTTTATGAAAAGCCAGGGGCTAAGCCAGCTCTACCATAACCAGAGTCAGG
  GACTCTTATCCCAGCTGCAAGGACAGTCGAAGGATATGCCACCTCGGTTTTCTAAGAAAGGACAGCTTAA
  TGCAGATGAGATTAGTTTGAGGCCTGCTCAGTCGTTTCTAATGAATAAAAATCAGGTGCCAAAGCTTCAG
  CCCCAGATAACTATGATTCCTCCCAGTGCACAGCCACCACGCACTCAAACACCACCTCTGGGACAGACAC
  CTCAACTTGGTCTCAAAACTAATCCACCACTTATTCAGGAAAAGCCTGCCAAGACTAGCAAAAAGCCACC
  ACCATCAAAGGAAGAACTACTTAAACTGACCGAAGCTGTTGTAACTGAGTATCTGAACAGTGGAAATGCC
  AACGACGCTGTCAGTGGTGTGAGAGAAATGAGAGCTCCAAAACACTTTCTTCCTGAGATGTTAAGCAAAG
  TGATCATCCTGTCACTTGATAGAAGTGATGAAGATAAAGAAAAAGCAAGCTCTTTGATCAGTTTACTCAA
  ACAGGAAGGGATAGCCACAAGTGACAACTTTATGCAGGCTTTCCTGAATGTATTGGAGCAGTGCCCCAAA
  CTGGAGGTTGACATCCCCTTGGTGAAATCTTACTTGGCACAGTTTGCGGCTCGTGCTATAATTTCAGAGT
  TGGTGAGCATTTCAGAACTAGCTCAACCACTGGAGAGTGGCACCCACTTCCCTCTCTTCTTACTTTGTCT
  TCAACAATTAGCTAAATTACAAGACCGAGAATGGTTAACCGAACTTTTTCAACAAAGCAAGGTCAATATG
  CAGAAAATGCTGCCAGAAATTGATCAGAATAAGGATCGAATGTTGGAGATTTTGGAAGGAAAGGGACTGA
  GTTTCTTATTCCCACTCCTTAAATTGGAGAAGGAACTATTGAAGCAAATTAAGCTGGATCCATCCCCTCA
  AACTATATATAAATGGATTAAAGATAACATCTCTCCCAAACTTCATGTAGATAAAGGATTTGTGAACATC
  TTAATGACCAGCTTCTTACAGTACATTTCTAGTGAAGTTAGCCCACCCAGCGATGAAACAGATTCTTCCT
  CTGCTCCTTCCAAAGAACAGTTAGAGCAGGAAAAACAGCTGCTGCTCTCTTTTAAGCCAGTGATGCAGAA
  GTTTCTTCATGATCATGTGGATCTACAGGTCAGTGCCCTGTATGCTTTGCAGGTGCACTGTTACAACAGC
  AGCTTCCCAAAAGGCATGTTACTTCGATTTTTTGTTCATTTCTATGACATGGAAATTATTGAAGAGGAAG
  CTTTCTTAGCTTGGAAGGAAGACATAACTCAAGAGTTTCCAGGAAAAGGCAAGGCTTTGTTCCAGGTGAA
  TCAGTGGCTAACCTGGCTAGAAACTGCTGAAGAAGAAGAATCAGAGGAAGAAGCTGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR209576 representing NM_001017374
  Red=Cloning site Green=Tags(s)
  
  VESAIAEGGASRFSASSGGGGSRGAPQHYPKTAGNSEFLGKTPGQNAQKWIPARSTRRDDNSAANNSANE
  KERHDAIFRKVRGILNKLTPEKFDKLCLELLNVGVESKLILKGVILLIVDKALEEPKYSSLYAQLCLRLA
  EDAPNFDGPAAEGQPGQKQSTTFRRLLISKLQDEFENRTRNVDVYDKRENPLLPEEEEQRAIAKIKMLGN
  IKFIGELGKLDLIHESILHKCIKTLLEKKKRVQLKDMGEDLECLCQIMRTVGPRLDHERAKSLMDQYFAR
  MCSLMLSKELPARIRFLLQDTVELREHHWVPRKAFLDNGPKTINQIRQDAVKDLGVFIPAPMAQGRNDFF
  LEGPFMPPRMKMDRDPLGGLADMFGQMPGSGIGTGPGVIQDRFSPTMGRHRSNQLFNGHGGHIMPPTQSQ
  FGEMGGKFMKSQGLSQLYHNQSQGLLSQLQGQSKDMPPRFSKKGQLNADEISLRPAQSFLMNKNQVPKLQ
  PQITMIPPSAQPPRTQTPPLGQTPQLGLKTNPPLIQEKPAKTSKKPPPSKEELLKLTEAVVTEYLNSGNA
  NDAVSGVREMRAPKHFLPEMLSKVIILSLDRSDEDKEKASSLISLLKQEGIATSDNFMQAFLNVLEQCPK
  LEVDIPLVKSYLAQFAARAIISELVSISELAQPLESGTHFPLFLLCLQQLAKLQDREWLTELFQQSKVNM
  QKMLPEIDQNKDRMLEILEGKGLSFLFPLLKLEKELLKQIKLDPSPQTIYKWIKDNISPKLHVDKGFVNI
  LMTSFLQYISSEVSPPSDETDSSSAPSKEQLEQEKQLLLSFKPVMQKFLHDHVDLQVSALYALQVHCYNS
  SFPKGMLLRFFVHFYDMEIIEEEAFLAWKEDITQEFPGKGKALFQVNQWLTWLETAEEEESEEEAD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001017374
• ORF Size: 2718 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001017374.2, NP_001017374.2
• RefSeq Size: 5008 bp
• RefSeq ORF: 2721 bp
• Locus ID: 361628
• Cytogenetics: 1q33
• MW: 102.1 kDa
• Gene Summary: Translation initiation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F (eIF4F), which is a cap binding protein complex that consists of three subunits: eIF4A, eIF4E and eIF4G. The protein encoded by this gene shares similarity with the C-terminal region of eIF4G that contains the binding sites for eIF4A and eIF3; eIF4G, in addition, contains a binding site for eIF4E at the N-terminus. Unlike eIF4G, which supports cap-dependent and independent translation, this gene product functions as a general repressor of translation by forming translationally inactive complexes. In vitro and in vivo studies in human indicate that translation of this mRNA initiates exclusively at a non-AUG (GUG) codon. This also appears to be true for mouse and rat. [provided by RefSeq, Jul 2008]