Txnrd3 (NM_001106609) Rat Untagged Clone

CAT#: RN217002

Txnrd3 (untagged) - Rat thioredoxin reductase 3 (Txnrd3), transcript variant 1

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Txnrd3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN217002 representing NM_001106609
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGTCGCCACCCGGCCGCCGCGCCCGCCTGACGTCCCCCGGGACCAGCCGCCCGCCCGCTGAGGTCC
  GCGAGGAGGTGCGGCGCCGCCTGCGGGATCTCATCGAGGGCAACAGGGTTATGATCTTCAGCAAGAGTTA
  CTGTCCACACAGCTCGCGGGTTAAGGAACTCTTTTCATCCTTGGGAGTGAACTATTACATCCTGGAACTT
  GATCAAGTTGATGATGGGGCCAATGTTCAGGAAGTGCTGACAGAAATCAGTAACCAGAAAACGGTGCCCA
  ATATTTTTGTGAATAAAGTGCACGTGGGTGGATGTGACCGGATTTTCCAGGCACATCAGAATGGTTTACT
  GCAGAAGCTCCTTCAGGATGACTCAACTCATGATTATGATCTCATCGTCATCGGCGGGGGCTCTGGCGGC
  CTCTCTTGTGCCAAGGAAGCTGCCAACTTGGGAAAGAAGGTCATGGTGCTAGACTTTGTGGTTCCCTCGC
  CTCAGGGCACGTCCTGGGGCCTTGGCGGCACCTGTGTGAATGTAGGCTGTATCCCTAAGAAGCTGATGCA
  TCAGGCAGCCCTCCTGGGGCATGCTCTGCAAGATGCGAGGAAATATGGCTGGGATTATAACCAGCAGGTG
  AAGCACAACTGGGAGACCATGAGGGAAGCGATCCAGAACCACATTGGCTCCTTGAACTGGGGCTACAGGG
  TAACTCTTCGGGAGAAAGGCGTGACCTATGTCAACTCCTTCGGGGAGTTTGTGGAACTGCATAAAATAAA
  GGCAACCAATAAGAAAGGGCAGGAAACTTTTTACACCGCTTCGAAGTTTGTCATAGCAACAGGGGAAAGG
  CCGCGGTACTTGGGAATCCAGGGAGATAAAGAGTACTGTATTACCAGCGACGACCTGTTCTCTCTGCCAT
  ACTGCCCAGGGAACACATTAATCGTTGGCGCCTCGTATGTTGGTCTGGAGTGTGCAGGCTTTTTGGCTGG
  CTTGGGGCTAGATGTGACAGTCATGGTACGCTCTGTCCTTCTTCGTGGCTTTGATCAAGAAATGGCAGAG
  AAAGTGGGATCCTACCTGGAACAACAAGGCGTCAAGTTCCAAAGGAAATTCACCCCCATTTTGGTTCAAC
  AGTTGGAGAAAGGCTTACCCGGAAGATTGAAAGTCGTGGCTAAGTCCACTGAAGGACCAGAGACAGTAGA
  AGGGACATACAACACGGTTTTGTTAGCAATTGGTCGTGACTCCTGTACAAGGAAAATAGGGCTGGAGAAG
  ATCGGGGTCAAAATCAATGAGAAGAATGGCAAAATACCAGTAAATGATGTGGAACAGACCAATGTGCCTC
  ATGTTTATGCTATTGGGGACGTACTGGAGGGCAAACCAGAGCTCACACCTGTTGCCATACAGGCAGGCAA
  GCTGCTAGCTCGAAGACTCTTTGGGATCTCTTTAGAAAAGTGTGATTACATTAACGTCCCAACAACGGTG
  TTCACACCTCTGGAATACGGCTGTTGTGGACTGTCTGAAGAGAAAGCCATTGAAATGTATACGAAGGAGA
  ATCTGGAAGTGTATCACACCTTCTTTTGGCCTCTTGAGTGGACAGTTGCTGGCAGAGACAACAACACCTG
  TTATGCAAAGATAATCTGCAACAAATTTGACAATGACCGTGTGATAGGATTTCACCTCCTGGGGCCAAAT
  GCTGGTGAAGTCACACAGGGGTTTGCAGCTGCAATGAAATGTGGGCTTACGAAGCAGCTACTGGATGACA
  CCATTGGAATCCACCCCACCTGTGGTGAGGTATTTACAACCATGGAAATCACAAAGTCCTCAGGGCTGGA
  CATTACTCAGAAAGGCTGCTGAGGCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001106609
• ORF Size: 1848 bp
• Insert Size: 1848
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This clone expresses the complete ORF with c-terminal tags of Myc-DDK.

Reference Data

• RefSeq: NM_001106609.2, NP_001100079.2
• RefSeq Size: 2799
• RefSeq ORF: 1848
• Locus ID: 297437
• Gene Summary: The protein encoded by this gene belongs to the pyridine nucleotide-disulfide oxidoreductase family, and is a member of the thioredoxin (Trx) system. Three thioredoxin reductase (TrxR) isozymes are found in mammals. TrxRs are selenocysteine-containing flavoenzymes, which reduce thioredoxins, as well as other substrates, and play a key role in redox homoeostasis. This gene encodes the third TrxR, which unlike the other two isozymes, contains an additional N-terminal glutaredoxin (Grx) domain, and shows highest expression in testis. The Grx domain allows this isozyme to participate in both Trx and glutathione systems. It functions as a homodimer containing FAD, and selenocysteine (Sec) at the active site. Sec is encoded by UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. There is evidence for two isoforms resulting from the use of a non-AUG (CUG), and an in-frame downstream AUG as translation initiation codons (PMID:20018845). [provided by RefSeq, Aug 2017]
  Transcript Variant: This variant (1) contains two in-frame translation initiation codons: an upstream non-AUG (CUG) and a downstream AUG codon. The isoform (2) represented in this RefSeq results from the use of the AUG start codon. It has a shorter N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript and the encoded protein are supported by orthologous mouse data.