Rtn3 (NM_001009953) Rat Tagged ORF Clone

CAT#: RR200210

Rtn3 (Myc-DDK-tagged ORF) - Rat reticulon 3 (Rtn3), transcript variant 2, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rtn3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200210 representing NM_001009953
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGTCATCGGCGGCCACTCAGTCTCCGTCAGTCTCCTCGTCGTCCTCCGGGGCCGAGCCGTCAA
  CGCTTGGCGGCGGCGGCGGGAGCCCTGGAGCCTGCCCCGCCCTGGGGGCGAAGAGCTGCGGCTCCTCCTG
  TGCGGTGCATGATCTGATTTTCTGGCGAGATGTGAAGAAGACTGGGTTTGTCTTTGGCACCACGCTGATC
  ATGCTGCTCTCTCTGGCAGCTTTCAGTGTTATCAGTGTGGTCTCTTACCTCATCCTGGCTCTACTCTCTG
  TCACCATCAGCTTCAGAGTCTACAAGTCTGTCATCCAAGCTGTGCAGAAGTCAGAAGAAGGACATCCATT
  CAAGGCCTACCTGGATGTGGACATTACACTGTCCTCAGAAGCTTTCCACAGCTACATGAATGCTGCAATG
  GTGCATGTCAACAAGGCCCTCAAACTCATTATTCGTCTCTTTCTGGTAGAAGACTTGGTTGACTCCTTGA
  AGCTGGCTGTCTTCATGTGGCTGATGACCTACGTCGGTGCTGTTTTTAACGGAATTACCCTTCTGATTCT
  CGCCGAGCTGCTGGTTTTCAGCGTCCCAATTGTCTATGAGAAGTATAAGACACAGATTGACCACTATGTT
  GGGATTGCCCGGGATCAGACCAAGTCAATTGTTGAAAAGATCCAAGCAAAGCTTCCTGGAATCGCCAAAA
  AAAAGGCAGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200210 representing NM_001009953
  Red=Cloning site Green=Tags(s)
  
  MAESSAATQSPSVSSSSSGAEPSTLGGGGGSPGACPALGAKSCGSSCAVHDLIFWRDVKKTGFVFGTTLI
  MLLSLAAFSVISVVSYLILALLSVTISFRVYKSVIQAVQKSEEGHPFKAYLDVDITLSSEAFHSYMNAAM
  VHVNKALKLIIRLFLVEDLVDSLKLAVFMWLMTYVGAVFNGITLLILAELLVFSVPIVYEKYKTQIDHYV
  GIARDQTKSIVEKIQAKLPGIAKKKAE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001009953
• ORF Size: 711 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001009953.1, NM_001009953.2, NM_001009953.3, NP_001009953.2
• RefSeq Size: 2817 bp
• RefSeq ORF: 714 bp
• Locus ID: 140945
• Cytogenetics: 1q43
• MW: 25.4 kDa
• Gene Summary: This gene encodes a member of the reticulon family, a large group of membrane-bound proteins that primarily localize to the endoplasmic reticulum with a small subpopulation at the cell surface. These proteins play roles in endocytosis, exocytosis, trafficking and neuronal outgrowth. Proteins belonging to this family are characterized by a carboxy-terminal reticulon homology domain that consists of a small hydrophilic loop flanked by hydrophobic regions. In rat, this protein has been shown to interact with two members of the synaptic adhesion-like molecule family, suggesting that it may function in trafficking of these proteins. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2015]