Dkc1 (NM_133419) Rat Tagged ORF Clone

CAT#: RR200986

Dkc1 (Myc-DDK-tagged ORF) - Rat dyskeratosis congenita 1, dyskerin (Dkc1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dkc1
• Synonyms: Nap57
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200986 representing NM_133419
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGACGCGGAAGCCGCCATGACGTTCCCGAAGAAGCACAAGAAGAAGAAAGAGCGGAAGCCGCTGC
  CGGAAGCTGACGTGGCTGAGATCCAGCACGCGGAAGATTTCCTGATCAAACCGGAGTCCAAGGCGGCTCA
  GTTGGACACCTCCCAGTGGCCCCTGCTGCTCAAGAACTTTGACAGACTGAACGTGCGGACGACCCACTAC
  ACGCCCATCCCGTGCGGCTCCAACCCGCTGAAGAGGGAGATCGGGGAGTACGTCAGGACCGGCTTCATCA
  ACCTGGACAAGCCATCCAACCCCTCTTCGCACGAGGTCGTGGCCTGGATCCGCCGCATCCTGCGCGTGGA
  GAAGACGGGGCACAGCGGGACACTTGACCCCAAGGTCACCGGGTGCCTCATCGTCTGCATCGAGCGCGCC
  ACGCGCCTGGTCAAGTCACAGCAGAGCGCGGGCAAAGAGTATGTCGGGGTCGTGCGGCTGCACAATGCTA
  TCGAGGGGACCGCCCAGCTCTCCAGGGCCCTGGAGACCCTGACAGGCGCCCTGTTCCAGCGTCCCCCGCT
  CATCGCAGCAGTGAAGCGGCAGCTGCGCGTGCGCACAATCTATGAGAGCCGTGTTGTCGAGTATGACCCT
  GAGCGCAGGCTGGGTGTGTTCTGGGTGAGCTGCGAGGCTGGCACCTACATCCGGACGCTGTGCGTGCACC
  TGGGGCTGCTGCTGGGCGTCGGGGGGCAGATGCAGGAGCTGCGGCGCGTGCGCTCGGGGGTCGTGGGCGA
  GCGGGACCACATGGTGACCATGCACGACGTCCTGGACGCGCAGTATCTATACGACCACCACCGGGACGAG
  AGCTACCTGCGCCGCGTGGTTTTCCCACTGGAGAAGCTGCTGACGTCGCACAAGCGCCTGGTGATGAAGG
  ACAGTGCGGTCAACGCCATCTGCTACGGCGCGAAGATCATGCTCCCCGGCCTCCTGCGCTACGAGGACGG
  CATCGAGGTCAACCAGGAGGTCGTGGTCATCACCACCAAGGGCGAGGCCGTCTGCGTGGCCATCGCACTG
  ATGACGACGGCTGTGATCTCCACGTGCGACCACGGGGTAGTAGCGAAGATCAAGCGCGTCATCATGGAGC
  GGGACACCTACCCCCGCAAGTGGGGCCTGGGGCCGAAGGCAAGCCAGAAGAAGCAGCTGATCAAGCAGGG
  CCTCCTGGACAAACATGGCCGACCCACGGACGGCACCCCCGCCTCCTGGACGCGCGACTACGTGGACTAC
  AGTGACTCCAGCAAGAAAGCGACGGCCGCCGAAGCCACGCCGGGCCCTGGGGTCACCGCGGACGCCGCCA
  GCATCGTGAAAAGGAAACGCGATAGCGACAGCGACGCCGACGAGGCGACGCCCACTACCACGCCCCGGGT
  GAAGAAGGAGAAGAAGAAGAAAAAGGAGAAGGCGGACGGCGGGGAGGAGGCTGCGGAGGACGGGGACGGC
  GACGCCACAAGGAAGAAAAAGAAGAAGAAGGCAAGGGCGGCAGAGGAGCTCAGTGGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200986 representing NM_133419
  Red=Cloning site Green=Tags(s)
  
  MADAEAAMTFPKKHKKKKERKPLPEADVAEIQHAEDFLIKPESKAAQLDTSQWPLLLKNFDRLNVRTTHY
  TPIPCGSNPLKREIGEYVRTGFINLDKPSNPSSHEVVAWIRRILRVEKTGHSGTLDPKVTGCLIVCIERA
  TRLVKSQQSAGKEYVGVVRLHNAIEGTAQLSRALETLTGALFQRPPLIAAVKRQLRVRTIYESRVVEYDP
  ERRLGVFWVSCEAGTYIRTLCVHLGLLLGVGGQMQELRRVRSGVVGERDHMVTMHDVLDAQYLYDHHRDE
  SYLRRVVFPLEKLLTSHKRLVMKDSAVNAICYGAKIMLPGLLRYEDGIEVNQEVVVITTKGEAVCVAIAL
  MTTAVISTCDHGVVAKIKRVIMERDTYPRKWGLGPKASQKKQLIKQGLLDKHGRPTDGTPASWTRDYVDY
  SDSSKKATAAEATPGPGVTADAASIVKRKRDSDSDADEATPTTTPRVKKEKKKKKEKADGGEEAAEDGDG
  DATRKKKKKKARAAEELSG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133419
• ORF Size: 1527 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_133419.1, NP_596910.1
• RefSeq Size: 1803
• RefSeq ORF: 1530
• Locus ID: 170944
• MW: 56.6 kDa
• Gene Summary: nucleolar protein expressed in liver; colocalized with Nopp140 protein; may be involved with rRNA processing [RGD, Feb 2006]