B4galt3 (NM_001009539) Rat Tagged ORF Clone

CAT#: RR201013

  • TrueORF®

B4galt3 (Myc-DDK-tagged ORF) - Rat UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 3 (B4galt3), (10 ug)


  "NM_001009539" in other vectors (3)

Reconstitution Protocol

USD 420.00

4 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Type Rat Tagged ORF Clone
Tag Myc-DDK
Symbol B4galt3
Synonyms MGC72586
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RR201013 representing NM_001009539
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTGCGGAGGCTGCTGGAGAGGCCCTGCACACTGGCCCTGCTTGTGGGCTCCCAACTGGCAGTTATGA
TGTATCTGTCACTAGGGGGCTTCCGAAGTCTTAGTGCCCTGTTTGGTCGAGATCCAGGCCCAACATTCGA
CTATTCTCATCCCCATGATGTCTATAGTAACCTCAGTCACCTGCCTGGAGCCCCTGGAGCTGCAGGGGCT
CCTCTAGCTCAAGTTCTGCCTGACTGTCCAGAAAGATCGCCTTTCTTAGTGGGTCCTGTGTCAGTATCCT
TTAGCCCAGTGCCATCACTAGCAGAGATCGTGGAGCGGAATCCCCGGGTGGAATCAGGGGGCCGGTACCG
TCCTGCAGGATGTGAGCCTCGCTCCCGAACAGCCATAATTGTGCCCCACCGTGCCCGGGAGCACCACCTC
CGACTGCTACTCTATCACCTGCACCCCTTCCTGCAGCGCCAGCAGCTTGCTTATGGCATCTATGTCATCC
ACCAGGCTGGAAATGGAACGTTTAACAGGGCCAAACTGCTGAACGTAGGGGTCAGGGAAGCCCTTCGTGA
CGAAGAGTGGGACTGCTTGTTTTTACACGATGTGGACCTCCTTCCAGAAAACGACCATAACCTGTATGTG
TGTGACCCCCGGGGACCCCGCCATGTTGCTGTCGCCATGAACAAGTTTGGATACAGCCTTCCGTACCCCC
AGTACTTTGGCGGAGTTTCAGCACTCACTCCTGACCAGTACCTGAAGATGAATGGCTTCCCCAATGAATA
CTGGGGCTGGGGTGGCGAGGATGACGACATTGCTACCAGGGTTCGCCTGGCTGGGATGAAGATTTCCCGA
CCACCCACCTCTGTGGGACACTACAAGATGGTGAAGCACAGAGGGGATAAAGGAAATGAGGAAAACCCCC
ACAGATTTGACCTCCTGGTCCGTACCCAGAATTCTTGGACACAAGATGGAATGAACTCACTAACATACCG
ACTGCTGGCAAGAGAGCTGGGTCCTCTCTACACCAACATCACTGCAGACATTGGGACTGACCCTCGGGGT
CCCCGGGCTCCCTCTGGTCCCCGATACCCACCAGGTTCCTCCCAGGCCTTCCGTCAGGAGATGCTGCAAC
GCCGGCCCCCAGCTAGGCCTGGCCCTCTGCCTACTGCCAACCACACAGCTCCCCATGGTTCACAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RR201013 representing NM_001009539
Red=Cloning site Green=Tags(s)

MLRRLLERPCTLALLVGSQLAVMMYLSLGGFRSLSALFGRDPGPTFDYSHPHDVYSNLSHLPGAPGAAGA
PLAQVLPDCPERSPFLVGPVSVSFSPVPSLAEIVERNPRVESGGRYRPAGCEPRSRTAIIVPHRAREHHL
RLLLYHLHPFLQRQQLAYGIYVIHQAGNGTFNRAKLLNVGVREALRDEEWDCLFLHDVDLLPENDHNLYV
CDPRGPRHVAVAMNKFGYSLPYPQYFGGVSALTPDQYLKMNGFPNEYWGWGGEDDDIATRVRLAGMKISR
PPTSVGHYKMVKHRGDKGNEENPHRFDLLVRTQNSWTQDGMNSLTYRLLARELGPLYTNITADIGTDPRG
PRAPSGPRYPPGSSQAFRQEMLQRRPPARPGPLPTANHTAPHGSH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001009539
ORF Size 1185 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001009539.1, NP_001009539.1
RefSeq Size 2007 bp
RefSeq ORF 1188 bp
Locus ID 494342
Cytogenetics 13q24
MW 44 kDa

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.