Lamp3 (NM_001012015) Rat Tagged ORF Clone

CAT#: RR205961

Lamp3 (Myc-DDK-tagged ORF) - Rat lysosomal-associated membrane protein 3 (Lamp3), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Lamp3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR205961 representing NM_001012015
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTGGGCAGACCTCTGCAGTAGCTGTACTCCTGTGTCTGGCTGTAATTTTACATGGCTATCAAATAA
  GAGAAAAAGAATTTCCAGAAGCCAGAGGCTATCTTCAATATACTGCAACAACAACAGAACAGATCACAGC
  AAAACCTCCACTACCACTGACTAACCAAACATCTCATGCAACTTTAGCATCAAGGTCCAAGGATGATTAC
  ATTCAAACGGCTGCCGAAACTTCCACTTTTGAAGACACAGCCCACATAACAATGAAAACAGCAATCCCAG
  TAACTACGAAGAGCCTTCTACCCATCAGCTCAACTAGCTATACCTTTGTTAGAACTAATAACTCACACAT
  GACTGCTTCATCTACTGAAGATACTATTGGCTCTGGCTCAATTACACATTTACCATTTCCTACAACCAGA
  GCTAGTCTAGCTGCTGTCAATCATATAACTGGGAGAAGTACCCAACTTGGTGGTCAGACTACCCTTCCCA
  AAGCACTTTTCACACCATCACACGAAAGCACAACCACTCAGAGGCCAACTCTATCCACTATTGTCTCAGA
  ACTAACACCTACAGGCAAGGACAGATCAACAACTTCATCTGTTCCCTTAGTTCCCAGGCCTACTTTTGTA
  ACTTGGTCATCACCTGCCAAGATTGGAACTTATGAAGTACTAAATGGAAGCAGGCTCTGTATAAAAGCAG
  AGATGGGAATAGCGCTGATTGTTCAAGAAAAGGGTTTGGATTCTGCAACCCAGAGGCACTTCAACATTGA
  CCCCAGTCTAACCCATGCATCTGGGAAATGTGGCTCCCAAAACTCCAACCTCTTCTTGAATTTCCAGGGT
  GGATCCGTGAATGTCACATTTACCAAGGAAGAAAACTTATATTACGTTAGTGAAGTTGGAGCTTATTTGA
  CCATCTCAAATACAGAGAAGACCTACCAAGGAAAGAGTACTATGATGATGTTTGAGACAGTAGTTGGGCA
  TTCTTTCAAGTGTGTCAGTGAGCAGAGCATCCAGCTATCAGCTCAGCTTCAAATGAAAACAATGAATATC
  CATCTTCAGGCCTTTGATTTTGAAGGAGACTCCTTTGGAATTGTGGACGAGTGTTTGTCAGACTACACAG
  TTGTGCTTCCTGTGGTTGGGATCATCGTGGTTGTCCTCTGTGTTGTGGGTCTGGGCATCTACAAAATCCG
  CCAAAGGCGTCAGTCATCTGCATACCAGAGAATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR205961 representing NM_001012015
  Red=Cloning site Green=Tags(s)
  
  MPGQTSAVAVLLCLAVILHGYQIREKEFPEARGYLQYTATTTEQITAKPPLPLTNQTSHATLASRSKDDY
  IQTAAETSTFEDTAHITMKTAIPVTTKSLLPISSTSYTFVRTNNSHMTASSTEDTIGSGSITHLPFPTTR
  ASLAAVNHITGRSTQLGGQTTLPKALFTPSHESTTTQRPTLSTIVSELTPTGKDRSTTSSVPLVPRPTFV
  TWSSPAKIGTYEVLNGSRLCIKAEMGIALIVQEKGLDSATQRHFNIDPSLTHASGKCGSQNSNLFLNFQG
  GSVNVTFTKEENLYYVSEVGAYLTISNTEKTYQGKSTMMMFETVVGHSFKCVSEQSIQLSAQLQMKTMNI
  HLQAFDFEGDSFGIVDECLSDYTVVLPVVGIIVVVLCVVGLGIYKIRQRRQSSAYQRI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001012015
• ORF Size: 1224 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001012015.1, NP_001012015.1
• RefSeq Size: 1927 bp
• RefSeq ORF: 1227 bp
• Locus ID: 303801
• Cytogenetics: 11q23
• MW: 44.4 kDa