Eif4b (NM_001008324) Rat Tagged ORF Clone

CAT#: RR209279

Eif4b (Myc-DDK-tagged ORF) - Rat eukaryotic translation initiation factor 4B (Eif4b), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Eif4b
• Synonyms: MGC94923
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR209279 representing NM_001008324
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCTCAGCAAAAAAGAAGAATAAGAAGGGGAAGACCATCTCCCTAACAGACTTTCTAGCTGAGG
  ATGGGGGAACTGGTGGAGGAAGCACCTATGTCCCCAAACCAGTCAGCTGGGCTGATGAAACAGACGATCT
  GGAAGGAGATGTGTCAACAACTTGGCATAGTAACGATGACGATGTGTACAGGGCACCTCCTATTGACCGT
  TCCATCCTTCCCACTGCTCCACGGGCTGCTCGGGAACCCAATATTGATCGGAGCCGTCTTCCCAAGTCAC
  CACCCTACACTGCTTTCCTAGGGAATCTGCCCTATGATGTGACAGAAGACTCTATTAAGGATTTCTTTAG
  AGGATTAAATATCAGCGCTGTACGCTTGCCGCGTGAGCCCAGCAATCCAGACAGGTTGAAAGGTTTTGGC
  TATGCCGAATTTGAGGATCTGGATTCTCTGCTCAGTGCTCTGAGTCTCAATGAAGAGTCTCTAGGTAACA
  GGAGAATTCGGGTGGATGTTGCTGATCAAGCACAGGATAAAGACAGGGATGACCGTTCTTTTGGTCGAGA
  TAGAAATCGGGATTCTGACAAGACAGACACAGACTGGAGGGCCCGTCCTGCCACAGACAGCTTTGATGAC
  TACCCACCTAGACGAGGTGATGACAGCTTCGGAGACAAGTATCGAGATCGTTACGAGTCAGACCGGTATC
  GGGATGGGTATAGGGACGGATATCGGGACGGCCCACGCAGAGACATGGACCGCTATGGGGGCCGGGATCG
  CTATGATGACCGAGGCAGCAGAGACTATGACCGAGGCTATGACTCCAGGATAGGCAGTGGCAGAAGAGCA
  TTTGGAAGTGGGTACCGGAGGGATGACGACTACAGAGGAGGTGGGGACCGCTATGAAGATCGCTATGACA
  GACGGGACGATCGGTCATGGAGCTCCAGGGACGATTACTCTCGGGACGATTACAGGCGTGATGACAGAGG
  TCCCCCCCAAAGACCCAAACTGAATCTAAAGCCTCGGAGTACTCCTAAAGAAGATGATTCCTCTGCTAGC
  ACCTCCCAGTCCAGCCGAGCGGCTTCTATCTTTGGAGGGGCGAAGCCTGTTGACACAGCTGCTAGAGAAA
  GAGAAGTAGAGGAGCGGCTACAGAAGGAGCAGGAGAAGCTGCAGCGTCAGCTGGATGAGCCAAAACTAGA
  CCGCCGGCCCCGGGAGAGACACCCAAGTTGGCGAAGTGAAGAAACTCAGGAAAGAGAACGGTCGAGGACA
  GGAAGTGAGTCATCGCAGACTGGGACCTCAGCCACATCTGGCAGAAATACACGAAGGAGAGAGAGTGAGA
  AGTCTCTAGAAAATGAAACCCTCAATAAAGAAGAAGACTGTCACTCTCCAACCTCTAAGCCTCCTAAACC
  TGACCAGCCTCTAAAGGTAATGCCAGCCCCTCCACCAAAGGAGAATGCGTGGGTGAAGCGAAGCTCTAAC
  CCTCCTGCTCGATCTCAGAGCTCAGACACAGAGCAGCCGTCCCCTACAAGTGGTGGAGGGAAAGTTGCTC
  CAGCTCAGCCCTCTGAGGAAGGACCATCAAGGAAAGATGAAACTAAAGTGGATGGGGTGAGCACCACCAA
  AGGCCAGACTGGACACTCCAGCCGTGGTCCTGGGGATGGAGGGAGCAGAGACCACTGGAAGGAGTTGGAT
  AGGAAGGACGGCAAAAAAGATCAAGACTCCAGATCTGCACCTGAGCCAAAGAAATCTGAGGAGAACCGAG
  CCTCTAAGTTCAGTTCTGCAAGCAAGTACGCTGCTCTGTCTGTGGACGGTGAGGATGAGGATGAGGGAGA
  CGACTGCACTGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR209279 representing NM_001008324
  Red=Cloning site Green=Tags(s)
  
  MAASAKKKNKKGKTISLTDFLAEDGGTGGGSTYVPKPVSWADETDDLEGDVSTTWHSNDDDVYRAPPIDR
  SILPTAPRAAREPNIDRSRLPKSPPYTAFLGNLPYDVTEDSIKDFFRGLNISAVRLPREPSNPDRLKGFG
  YAEFEDLDSLLSALSLNEESLGNRRIRVDVADQAQDKDRDDRSFGRDRNRDSDKTDTDWRARPATDSFDD
  YPPRRGDDSFGDKYRDRYESDRYRDGYRDGYRDGPRRDMDRYGGRDRYDDRGSRDYDRGYDSRIGSGRRA
  FGSGYRRDDDYRGGGDRYEDRYDRRDDRSWSSRDDYSRDDYRRDDRGPPQRPKLNLKPRSTPKEDDSSAS
  TSQSSRAASIFGGAKPVDTAAREREVEERLQKEQEKLQRQLDEPKLDRRPRERHPSWRSEETQERERSRT
  GSESSQTGTSATSGRNTRRRESEKSLENETLNKEEDCHSPTSKPPKPDQPLKVMPAPPPKENAWVKRSSN
  PPARSQSSDTEQPSPTSGGGKVAPAQPSEEGPSRKDETKVDGVSTTKGQTGHSSRGPGDGGSRDHWKELD
  RKDGKKDQDSRSAPEPKKSEENRASKFSSASKYAALSVDGEDEDEGDDCTE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001008324
• ORF Size: 1833 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001008324.1, NP_001008325.1
• RefSeq Size: 3682 bp
• RefSeq ORF: 1836 bp
• Locus ID: 300253
• Cytogenetics: 7q36
• MW: 69.1 kDa