F8 (NM_183331) Rat Tagged ORF Clone

CAT#: RR209358

F8 (Myc-DDK-tagged ORF) - Rat coagulation factor VIII, procoagulant component (F8), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: F8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR209358 representing NM_183331
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCGCGGCGCGCGGCCTGTGCTTCTTCCTCAGCCTCTGCGCGCTCTGCTCCTGCGCCACCCGGAAGT
  ACTACCTGGGCGCCGTGGAGTTGCCATGGGACTATGTGAGCGGTGGTGCTTCCGGTGCCCAGCGCAGCGA
  CTCCAGGTTCCCTCCCGGGACACCAGCGCCGTCTGCGCATGTTGTGCACACGAAGACGGTGTTTGTGGAG
  TACATGGACCGGTTTTTCCACACGGCAAAGCCCAGGCCACTGTGGATGGGTCTCCTAGGCCCCACCATTT
  GGACCGAGGTTCACGACACTGTGGTCATCACACTCAAAAACATGGCTTCTCATCCCGTCAGTCTCCACGC
  TGTCGGTATGTCCTTCTGGAAGGCGTCCGAGGGCGCGGCATACGACGACCACAGCAGCCCGGCCGAGAAG
  GACGACGACAAAGTCCTCCCCGGCGAGAGCCACACTTACGCGTGGCAGGTCCTGGGGGAGAGCGGCCCAA
  TGGCTTCCGACCCCCCGTGCCTCACCTATGCCTACCTGTCTCATGTGGACCTCGTGAGGGACCTGAACTC
  GGGCCTCGTCGGGGCCCTGCTCGTGTGCAAAGAAGGCCGTCTGTCCGCAGAGAGAACACGGATGCCACCC
  CACTTCGTGCTGCTTTTTGCTGTCTTCGATGAAGGGCGGAGCTGGCACGCAGCAACACGTGACCCCGCGT
  CCACCGAGGCCCAGCCCACGGTGCACACTGTGAATGGCTACACGAACCGGACACTGCCGGGTCTGACCGG
  ATGCCGCGGAACATCCGTGTACTGGCACGTGATGGCGGTGGGCACCACGCCCGACATACACTCCATCCTC
  CTCGAAGGCCACACATTCTCTGTGAGGAGCCACCGTCAGGCTTCCCTGGAGATCTCACCTATGACTCTGC
  TCACGGCTCAGACGTGGCTGATGGACCTGGGGCGGTTCCTGCTCTTCTGTCACATCTCCTCCCACAGACA
  CGGTGGCATGGAAGCCTACGTCCAGGTGGACAACTGCCCCGAGGCGCCCCAGGGGCAGGGGAAAAGGGAC
  GAGGACACAGAGGACTATGATGATCTGGACTCGGAAATGGACGTCTTCACCTGGGACCCCGATGCCGCCC
  CCTTCGTCCAGGTCCGCTCAGCAGCCAAGAGACACCCCAAGACCTGGGTGCACTACATCGCAGCAGAGGA
  GGGGGACTGGGACTACGCGCCTGCGGCCGCCACCCTAGAAGACGGGAGCCTGCGGAACCGGTACCTGGGG
  CGCGGCCCTGGCCGCATCGGCAGCAAATATAAGAAAGTCAGGTTCGTGGCCTACACGGACGGCACCTTTC
  ACACGCGTGAGGCCACCCCGCGTGAAGCTGGGCTCCTAGGGCCACTGCTCTACGGGGAGGTCGGAGACTC
  ACTGTTGATTGTGTTCAAGAACCGAGCGAGCCGGGCATACAACATCCACCCTCATGGGATCCGTGACGTT
  GGCGCTGTGCATGCAGGGAGGCTGCCACGAGGGGTGAAGCACGTGAAGGATTTGCCGATCCGCCCAGGAG
  AGACGTTCAAGTACAGGTGGACGCTGACCGCAGAGGATGGGCCTGCAAGGTCGGATGCGCGGTGTGTGAC
  GCGCTACTATGCGAGCGCTGTGGATCCCGAGAGAGACCTGGCTTCCGGGCTGATTGGCCCCCTCCTCATC
  TGCTGTAAAGAATCTGTAGATCAAAGGGGGAACCAGATGATGTCAGACGAGAGGAACGTCATCCTGTTCT
  CCGTGTTTGATGAGAACCGGAGCTGGTACATCTCGGAGAACATGCGACGCTTCCTCCCCGACGAGGCACA
  TGTGCAGCTGCAGGACCCCGAGTTCGAGGCCTCCAACCTCATGCACAGCATTAATGGCTACGTCTTCGAT
  GGCCTGGAGCTGCGCGTTTGCCTGCACGAGGTGGCTGCCTGGCACGTGCTCAGCGTCGGCGCGCAGACTG
  ACTTCCTGTCTGTCTTCTTCTCTGGACACACGTTCAGACACAGGGCGGTGTACGCAGACACACTCACGGT
  GTTCCCGCACTCGGGGGTCACCGTCTTCATGTCCATGGATAACCCAGGTGTGTGGGTGCTGGGGTGCCAC
  AACCCGGACTTCCGGGAGAGCGGGATGACCGCACTGCTGAAGGTTTCCAGTTGTGACGAGGGCGTGAGTG
  ATTACTACGGAGAGACCTATGAGGGCGTTCTGGCCCCGTTGGCCAATGACAACGCCGTGGACCCCAGGAG
  CTTCTCCCAGAATTCAAATCATCTGCACAGCAGGAAAAAGAAAAGCAACACGCCGATGCGCTTCCCACTG
  AGCCATCCTACCCAGCATGGCTCCTTTTGGTCGGACAGCCAGGGAGATGTCCATGACGCTGTTCATAGAG
  CCCATTCACCCGCTGCAGCACACAGCAATGAAGGCCCAGCCGAAGCAGCCCAGCTCGAGCCCAAACCCCA
  TCCCGGTGAGAAAATGGCACTTCCTCCTTCTTGGTTAAGAGCAAAGAGAAGTCTGGTGACAACCATGGAA
  GCCAAGCGGAAGAAACTGGATCTCCAGGTCCAGGTTTCTGGTTTGCCCGACGACAGAACAACCGCAGTGG
  TTGCGCCGGACGATCCGATGGCGGCTTGTAAGAAGGCGGGTTCTTCAGGGTTTCCAGATGAATCGAGTCC
  CGCTGCATTGGGTAAGAAAATGTATCCCCGTATCAGGCCCCACGGACCTTTAAGCATCATTGAAGGAAAC
  AGGGATTCCAACTCGTCGGACTCGACTTTAATGTATAGGCTGGGAAGTCCACCAGGAGACGCCACATCAT
  GGACGGAGAACCGCAGAGGACTTGGCGAGAAGAGGTCTCACAGGGTCGCTTTCCTGGCCAGAGGTAACAC
  TTTACTCAGCGACGTTGATGAAAAATCGCATGCCCCCGGCCCAACATCAGTTGGGAACAGCACAGCAGCC
  GTGCAGGACACCATATTAGAGATCTGCAGTGAGATTCTCGAGGTCACACCTCCGATTCATGACAGGATAC
  TTTCAGACAAAAGTGCTACATATTTGAGACCGCACCGTACGCCCGACAGAATCACCTCGACGGAAAGGAA
  AGACATACGTCACGAAAAAGATGGAGATCTTGTTCCGCAGGATGCAGATGACACAAGCGCACCATTCTCG
  GAGGCGCCTTTCCTGTCAGAATCCACAGATTGGTTAAAAGAGGCCAATGGGGATAACTCCGCAAAACCTG
  AGCAAGAACCCAGTCCAGAGCAATTAGTGTATTTAATGTATATAAAGTGTATGGAAAATCAGAGTTTCTC
  GTCAGAGAAGAATAAAATGATAGCAGGGCAAGGTGGATTTACCAAGAACACAGGACTTGAAGACACGGTT
  TTTCCCAGAAAGACGAGTGTGTTTCTCACCACTGTGGCTAAGCGGCAAGAAAGTGGGAGGCACAACCAAG
  AAAACATTCCTCAGGCAGTGACAGAGAAGGAAGCGCCAATCGAAGAGAAGGGAGCTCTGCCCCAGGTGCA
  CATAGCGACTGGCTCTAAGAACTTCCCGAGAGACATGTTTGTACTCGGTACTGGGCAAAATATAAATCTC
  CACGAAGAGACATATGTACCAGTTCACAAAAACGTCCCATGGACAAGAAACCCAACAGACACAAGACAGA
  TTCCCATGGTTCATTTCTTTAAGAAAAGGAAGGAGGAGGAAACCAATTCAGGAGGCTTGGTAAATAAAAC
  GAGAGAAACGGTGAGAAACTACCCAAGCCAGAAGAATAGCGTTGCTCGACGTAGGGAACAGGCTTCGGGA
  CGAATCAAAGCATCCGCTCGTTGGCTTCCAGACGTAAACCGTTCAATCCAGAGTCTCCTTAAACAGATTG
  ACCACCGCAAGGAAAGGAAAAAGTTCATTATAGAATCTCGCTCCGCAGATTCTTCTGGGACTAAAAGTCC
  CACTCAGACCAATCATTCTCCCTCACACGTTGTGAAAATGTCAGCATTCCCACCGAGAGACATCCGAAGG
  ATCGAGTCGCGAGACAGTTCTCAGGTCTGGGTGTCGTCCTATGCTTACGACTTTGAGACAGGAAGTTCTA
  GAATTCGAGAGAGCAGTCCTTTCCTGAATGAAACTGAAACGGATAACCCTTCTCTCGCCGTGCCACCGCG
  GGAGAGGTTCGTGCGTCGAGGAAGGTTCGCCTCCCCAGAAAAAGTGAACACACACTCAGCGCCGTGTGAC
  AAACCCGAAAATGTTGTCTCCTCGGAACCAGTGTCGCCTGGCGAAGCTGTCACAGTCGCACTCCCTCCTC
  ACGTCTCCACTCAGGAGGAGGAACCTTTGCCTGCAGGAAGTAGCTGTGAGCGTCGCGGACATGTGGATCT
  CTTTCTCGAGATCTCTCTCCAGAGAACACGGGGGCCCGTTGAACGGAGTAGAGGAAGGAGGTCTGGGGGA
  GACACGGAAGGTCACACAGAGAACCCCGGAAAGACTCCCTCCCCACTGCCACAGATGCCGAAAGATCAGC
  GTGGGTCTGAAGCGGGGTACCCGAAAATCTGCCGCTGGAGAGACGGCACTGTTTTGCCCCCGAGGCCTCA
  CAACCTTTCCCTAGGAGCAAAGAAAAAACCAAATCTGCCCCGAAGAGAAGCCACTCGGGTGGAGCAAGGC
  GAGGCTCCGAGCCTGGTCTCTCCAGAGCCCCTGGTGCTGAGACGACGTCCAAGGGAAGCTAGCACCCTTC
  TGCCAGGAGGAGAGATGCGAGAGGACGACGGTGATGGTGTCACGGCGGAGTACGACGACGACGATGACGT
  CATGGCAGAGTATGACGACGATGCTGTCACGGTGGACACGCCCGAGGATTTCGACATCTATGGCGAGGAG
  GCAGGGCAGGGACCCCGCGGCTTTCAGCAGAAAACGCGGCACTATTTCATCGCGGCCGTGGAGGAGCTTT
  GGGACTACGGGGTGCGTGTGTCGCCTGGAGCTCTGGGAGACAGGGCGTGGAGTGGGGACGCAGCTCGGTT
  CCGGAAAGTGGTTTTCCGTGAGTTCACGGACGGGTCGTTCACGCAGCGTGTGCACCGTGGGGAGCTGGAC
  GCACACCTGGGGCTATTGGGGCCATACATACGGGCGGAAGTGGAGGACAACATCGTGGTGACCTTCCGGA
  ACCAGGCCTCCCGCCCCTACTCCTTCTACTCCAGCCTCGTGTCCTACCCGGAAGCCGACGGTGGAGCTGC
  ACCAAGGAGCAACTTTGTGCGGCCCAACGAGACCAAAACTTACTTCTGGAGAGTCCGGCCCCACATGGCG
  CCCACGGACGGCGAGTTTGACTGCAAGGCCTGGGCCTATTTCTCCGACGTGGACCTCGAGCGAGACCTGC
  ACTCGGGGCTGATTGGGCCACTGCTCGTGTGCCACGCCTCCACCCTGCACCCCTCGCACGCGCGGCCGCT
  CGCAGTGCAGGAGTTCGCGCTGCTCTTCGCGGTGTTCGATGAGACCAAGAGCTGGTACTGCGCTGAGAAC
  CTGGACCGGAAGTGCAGGCCGCCCCGTGGCACCCCCGCGGGAGACCCCGCCTGGAGAGAAGAGTACCGCT
  TCCATGCCGTGAATGGTTACGTCGCAGATGCTCTGCTTGGCCTGGTGATGGCGGAAGGTCGGAGGACGAG
  GTGGCATCTGCTCAGCATGGGCGACCCCGGGCACGCACAGTCCGTGCATTTCAGCGCGCACAGCGTCACC
  GTGCGGGACGGGGGCGAGCACAGGACGGCGGTGTGCAACCTGTACCCAGGCGTGTTCACAACCGTGGAGA
  TGTTGCCGTCCAGAGCGGGGATCTGGCGGGTGGAGAGCCTGGTGGGCGAGCACCTACGGGCTGGGATGAG
  CGCCCTCTTCCTGGTGTACAGCACGCGCTGCCAGGTCCCGCTCGGCATGGCTTCCGGTTACATCCGGGAC
  TCGCAGATCACGGCTTCCGGCTACCACGGGCTGTGGACCCCGCAGCTCGCGAGGCTTCACAACGCGGGAT
  CCGTGAATGCCTGGAGTGCCAAGGAGCCATTCGCGTGGATCAAGGTGGATCTCCTGGCGCCCATGATTCT
  TCACGGCATCGAGACGCAGGGCGCCCGTCACAGACTTTCCAGCCTTTATGTGTCTCAGTTCATCATCATG
  TACAGCCTGGATGGGAAGCAGTGGCTGAGCTACCGAGGAAACTCCACGGGAAGCCTGATGGTTTTCTTCG
  GCAACGTGGACGCGTCCACTGTGAGGCACAACCGCTTCGACCCCCCGATCGTTGCCCGCTACATCCGTGT
  GCACCCCACACATGCCAGCATCCGTACCGCCCTGCGCATGGAGCTGCTCGGCTGCGACCTGAACAGCTGC
  AGCGCGCCCCTGGGCATGGAGAGTGGATGGATCTCGGACACGCAGGTCACTGCCTCGTCCCACCTCAGCA
  CCACGTTCTCTGCTTGGCCGCCTGCGCTCGCTCGGCTTCACCTCCGGGGTGGGGCCAACGCCTGGCGACC
  CCAGGTGAATGATCCCACACAGTGGCTGCAGGTGGACTTGCAGAGGACTGTGAAAGTCACCGGCGTCGTC
  ACCCAGGGCGCGCGGTCTCTCCTCACTGCCATGTTTGTGAAAAAGTTCCTCGTCTCCACCAGCCAAGATG
  GCCGCCACTGGACTCACGTCTTGCAAGACGGCAAGGTCAAGGTTTTTCAGGGTAACCGGGATGCGTCCAC
  GCCCATGGTGAACTCTCTGCACCCCCCGCGCTTCACTCGATACCTTCGGATTCACCCCCAAGTGTGGGAG
  CGGCAAATTGCTCTGCGGCTCGAGATCCTGGGGTGTGAGGCTCAGCAGCTGGAC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR209358 representing NM_183331
  Red=Cloning site Green=Tags(s)
  
  MRAARGLCFFLSLCALCSCATRKYYLGAVELPWDYVSGGASGAQRSDSRFPPGTPAPSAHVVHTKTVFVE
  YMDRFFHTAKPRPLWMGLLGPTIWTEVHDTVVITLKNMASHPVSLHAVGMSFWKASEGAAYDDHSSPAEK
  DDDKVLPGESHTYAWQVLGESGPMASDPPCLTYAYLSHVDLVRDLNSGLVGALLVCKEGRLSAERTRMPP
  HFVLLFAVFDEGRSWHAATRDPASTEAQPTVHTVNGYTNRTLPGLTGCRGTSVYWHVMAVGTTPDIHSIL
  LEGHTFSVRSHRQASLEISPMTLLTAQTWLMDLGRFLLFCHISSHRHGGMEAYVQVDNCPEAPQGQGKRD
  EDTEDYDDLDSEMDVFTWDPDAAPFVQVRSAAKRHPKTWVHYIAAEEGDWDYAPAAATLEDGSLRNRYLG
  RGPGRIGSKYKKVRFVAYTDGTFHTREATPREAGLLGPLLYGEVGDSLLIVFKNRASRAYNIHPHGIRDV
  GAVHAGRLPRGVKHVKDLPIRPGETFKYRWTLTAEDGPARSDARCVTRYYASAVDPERDLASGLIGPLLI
  CCKESVDQRGNQMMSDERNVILFSVFDENRSWYISENMRRFLPDEAHVQLQDPEFEASNLMHSINGYVFD
  GLELRVCLHEVAAWHVLSVGAQTDFLSVFFSGHTFRHRAVYADTLTVFPHSGVTVFMSMDNPGVWVLGCH
  NPDFRESGMTALLKVSSCDEGVSDYYGETYEGVLAPLANDNAVDPRSFSQNSNHLHSRKKKSNTPMRFPL
  SHPTQHGSFWSDSQGDVHDAVHRAHSPAAAHSNEGPAEAAQLEPKPHPGEKMALPPSWLRAKRSLVTTME
  AKRKKLDLQVQVSGLPDDRTTAVVAPDDPMAACKKAGSSGFPDESSPAALGKKMYPRIRPHGPLSIIEGN
  RDSNSSDSTLMYRLGSPPGDATSWTENRRGLGEKRSHRVAFLARGNTLLSDVDEKSHAPGPTSVGNSTAA
  VQDTILEICSEILEVTPPIHDRILSDKSATYLRPHRTPDRITSTERKDIRHEKDGDLVPQDADDTSAPFS
  EAPFLSESTDWLKEANGDNSAKPEQEPSPEQLVYLMYIKCMENQSFSSEKNKMIAGQGGFTKNTGLEDTV
  FPRKTSVFLTTVAKRQESGRHNQENIPQAVTEKEAPIEEKGALPQVHIATGSKNFPRDMFVLGTGQNINL
  HEETYVPVHKNVPWTRNPTDTRQIPMVHFFKKRKEEETNSGGLVNKTRETVRNYPSQKNSVARRREQASG
  RIKASARWLPDVNRSIQSLLKQIDHRKERKKFIIESRSADSSGTKSPTQTNHSPSHVVKMSAFPPRDIRR
  IESRDSSQVWVSSYAYDFETGSSRIRESSPFLNETETDNPSLAVPPRERFVRRGRFASPEKVNTHSAPCD
  KPENVVSSEPVSPGEAVTVALPPHVSTQEEEPLPAGSSCERRGHVDLFLEISLQRTRGPVERSRGRRSGG
  DTEGHTENPGKTPSPLPQMPKDQRGSEAGYPKICRWRDGTVLPPRPHNLSLGAKKKPNLPRREATRVEQG
  EAPSLVSPEPLVLRRRPREASTLLPGGEMREDDGDGVTAEYDDDDDVMAEYDDDAVTVDTPEDFDIYGEE
  AGQGPRGFQQKTRHYFIAAVEELWDYGVRVSPGALGDRAWSGDAARFRKVVFREFTDGSFTQRVHRGELD
  AHLGLLGPYIRAEVEDNIVVTFRNQASRPYSFYSSLVSYPEADGGAAPRSNFVRPNETKTYFWRVRPHMA
  PTDGEFDCKAWAYFSDVDLERDLHSGLIGPLLVCHASTLHPSHARPLAVQEFALLFAVFDETKSWYCAEN
  LDRKCRPPRGTPAGDPAWREEYRFHAVNGYVADALLGLVMAEGRRTRWHLLSMGDPGHAQSVHFSAHSVT
  VRDGGEHRTAVCNLYPGVFTTVEMLPSRAGIWRVESLVGEHLRAGMSALFLVYSTRCQVPLGMASGYIRD
  SQITASGYHGLWTPQLARLHNAGSVNAWSAKEPFAWIKVDLLAPMILHGIETQGARHRLSSLYVSQFIIM
  YSLDGKQWLSYRGNSTGSLMVFFGNVDASTVRHNRFDPPIVARYIRVHPTHASIRTALRMELLGCDLNSC
  SAPLGMESGWISDTQVTASSHLSTTFSAWPPALARLHLRGGANAWRPQVNDPTQWLQVDLQRTVKVTGVV
  TQGARSLLTAMFVKKFLVSTSQDGRHWTHVLQDGKVKVFQGNRDASTPMVNSLHPPRFTRYLRIHPQVWE
  RQIALRLEILGCEAQQLD
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_183331
• ORF Size: 6774 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_183331.1, NP_899160.1
• RefSeq Size: 8326
• RefSeq ORF: 6777
• Locus ID: 302470
• MW: 251.3 kDa
• Gene Summary: human homolog is a cofactor for factor IXa; involved in blood coagulation [RGD, Feb 2006]