Atp1a2 (NM_012505) Rat Tagged ORF Clone

CAT#: RR210998

Atp1a2 (Myc-DDK-tagged ORF) - Rat ATPase, Na+/K+ transporting, alpha 2 polypeptide (Atp1a2), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Atp1a2
• Synonyms: RATATPA2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR210998 representing NM_012505
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTCGTGGGGCAGGGCGTGAGTACTCGCCTGCCGCCACCACTGCGGAGAATGGGGGTGGCAAGAAGA
  AACAGAAAGAGAAGGAGCTCGATGAGCTGAAGAAGGAGGTTGCCATGGATGACCACAAGCTGTCCTTGGA
  TGAGCTGGGCCGAAAATACCAAGTGGATCTGTCCAAGGGCCTCACCAACCAGCGAGCTCAGGATATTCTG
  GCTAGAGACGGACCCAACGCCCTCACTCCACCCCCTACAACTCCTGAGTGGGTCAAGTTCTGCCGTCAGC
  TTTTTGGGGGCTTCTCTATCCTGCTGTGGATTGGGGCGCTTCTCTGCTTCTTAGCCTATGGTATCCTGGC
  CGCCATGGAGGACGAACCATCCAATGACAATTTATATCTAGGTATCGTGCTAGCAGCTGTAGTTATCGTC
  ACTGGCTGCTTCTCCTACTACCAGGAAGCCAAAAGCTCCAAGATTATGGACTCCTTCAAGAACATGGTGC
  CTCAGCAAGCTCTGGTCATCCGAGAGGGAGAGAAGATGCAGATCAATGCAGAGGAGGTGGTCGTGGGAGA
  CCTGGTGGAAGTGAAGGGTGGAGACCGTGTCCCCGCTGACCTCCGGATCATCTCCTCCCACGGTTGCAAG
  GTGGATAACTCATCCCTGACAGGGGAGTCGGAGCCCCAGACCCGGTCCCCTGAGTTCACCCATGAGAATC
  CCTTGGAGACCCGCAATATCTGTTTCTTCTCTACCAACTGTGTGGAAGGCACTGCCAGGGGCATTGTGAT
  CGCCACAGGTGACCGGACGGTGATGGGCCGCATAGCCACTCTTGCCTCTGGCCTAGAGGTGGGACAGACG
  CCGATAGCCATGGAGATCGAGCATTTCATCCAGCTGATCACGGGGGTGGCCGTGTTCCTGGGGGTCTCCT
  TCTTTGTTCTGTCGCTCATCCTGGGCTACAGCTGGCTGGAGGCTGTCATCTTCCTCATCGGCATCATCGT
  AGCCAACGTCCCCGAAGGGCTCTTGGCCACTGTTACTGTGTGCCTGACGCTGACAGCCAAGCGCATGGCT
  CGCAAGAACTGCCTGGTGAAGAACCTGGAGGCGGTGGAGACGCTGGGCTCCACGTCCACCATCTGCTCGG
  ACAAGACAGGCACCCTCACCCAGAACCGCATGACGGTGGCTCACATGTGGTTTGACAACCAGATCCATGA
  GGCTGACACCACTGAAGATCAGTCTGGGGCCACTTTTGACAAGCGGTCCCCGACGTGGACAGCCCTGTCT
  CGGATCGCTGGTCTCTGCAATCGTGCCGTCTTCAAGGCTGGGCAGGAGAACATCTCCGTGTCTAAGCGGG
  ACACAGCTGGTGACGCCTCTGAGTCAGCTCTGCTCAAGTGCATCGAGTTGTCCTGTGGCTCAGTGAGGAA
  GATGAGGGACAGGAATCCCAAGGTGGCAGAAATTCCCTTCAACTCTACCAACAAATATCAGCTTTCCATC
  CATGAGAGGGAAGACAGCCCCCAGAGCCATGTGCTGGTGATGAAAGGTGCCCCGGAGCGCATCCTGGACC
  GATGCTCTACCATCCTGGTACAGGGCAAGGAGATCCCTCTTGACAAGGAGATGCAAGATGCCTTTCAAAA
  CGCCTACATGGAGCTGGGAGGACTCGGGGAGCGAGTGCTGGGCTTCTGTCAGCTGAACCTGCCTTCTGGA
  AAGTTTCCTCGGGGCTTCAAATTTGACACGGATGAGCTGAACTTTCCCACAGAGAAGCTCTGCTTTGTGG
  GGCTCATGTCTATGATTGATCCCCCCAGAGCAGCTGTGCCAGATGCTGTGGGCAAGTGCAGAAGTGCAGG
  CATCAAGGTGATCATGGTGACTGGGGATCACCCTATCACAGCCAAGGCCATTGCCAAAGGTGTGGGCATC
  ATATCAGAGGGTAACGAGACTGTGGAAGACATTGCAGCCAGGCTCAACATTCCTGTGAGTCAAGTCAATC
  CCAGAGAAGCCAAGGCATGTGTAGTGCACGGCTCAGACCTGAAGGACATGACTTCAGAGCAGCTGGATGA
  GATCCTCAGGGACCACACGGAGATCGTGTTTGCCCGGACCTCCCCTCAGCAGAAGCTCATCATTGTGGAG
  GGCTGTCAGAGGCAGGGAGCCATCGTGGCAGTGACTGGTGACGGGGTGAACGACTCCCCCGCGCTGAAGA
  AGGCTGACATTGGCATTGCCATGGGCATCTCTGGCTCTGATGTCTCTAAGCAGGCAGCTGACATGATCCT
  TCTCGACGACAACTTTGCCTCCATTGTGACGGGCGTGGAGGAGGGGCGCCTGATCTTTGACAACCTGAAG
  AAGTCCATCGCGTACACCCTGACCAGCAACATCCCTGAGATCACCCCCTTCCTGCTGTTCATCATTGCCA
  ACATCCCCCTTCCCCTGGGCACCGTGACCATCCTGTGCATCGACCTGGGCACAGACATGGTTCCTGCCAT
  CTCATTAGCATACGAAGCGGCTGAGAGCGACATCATGAAGAGGCAGCCACGGAACTCCCAGACGGACAAG
  CTGGTGAACGAGAGGCTTATCAGCATGGCTTACGGACAGATCGGCATGATCCAGGCTCTGGGCGGCTTCT
  TCACCTACTTTGTAATACTGGCAGAGAACGGCTTCCTGCCATCGAGGCTGCTTGGGATCCGCCTTGACTG
  GGATGATCGGACTACCAACGACCTGGAGGACAGCTATGGACAAGAGTGGACCTATGAGCAGCGGAAGGTG
  GTGGAGTTCACATGCCACACGGCCTTCTTTGCCAGCATCGTGGTTGTGCAGTGGGCTGACCTCATCATTT
  GCAAGACCCGGCGCAACTCGGTGTTCCAGCAGGGCATGAAGAACAAGATCCTGATTTTTGGGCTGCTAGA
  AGAGACGGCTCTGGCTGCCTTCCTGTCTTACTGCCCGGGTATGGGGGTGGCCCTCCGAATGTACCCACTC
  AAGGTCACGTGGTGGTTCTGTGCCTTTCCCTACAGTCTCCTCATCTTCATCTATGATGAAGTCCGAAAGC
  TCATCCTGCGGCGGTACCCTGGGGGCTGGGTGGAGAAGGAGACGTACTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR210998 representing NM_012505
  Red=Cloning site Green=Tags(s)
  
  MGRGAGREYSPAATTAENGGGKKKQKEKELDELKKEVAMDDHKLSLDELGRKYQVDLSKGLTNQRAQDIL
  ARDGPNALTPPPTTPEWVKFCRQLFGGFSILLWIGALLCFLAYGILAAMEDEPSNDNLYLGIVLAAVVIV
  TGCFSYYQEAKSSKIMDSFKNMVPQQALVIREGEKMQINAEEVVVGDLVEVKGGDRVPADLRIISSHGCK
  VDNSSLTGESEPQTRSPEFTHENPLETRNICFFSTNCVEGTARGIVIATGDRTVMGRIATLASGLEVGQT
  PIAMEIEHFIQLITGVAVFLGVSFFVLSLILGYSWLEAVIFLIGIIVANVPEGLLATVTVCLTLTAKRMA
  RKNCLVKNLEAVETLGSTSTICSDKTGTLTQNRMTVAHMWFDNQIHEADTTEDQSGATFDKRSPTWTALS
  RIAGLCNRAVFKAGQENISVSKRDTAGDASESALLKCIELSCGSVRKMRDRNPKVAEIPFNSTNKYQLSI
  HEREDSPQSHVLVMKGAPERILDRCSTILVQGKEIPLDKEMQDAFQNAYMELGGLGERVLGFCQLNLPSG
  KFPRGFKFDTDELNFPTEKLCFVGLMSMIDPPRAAVPDAVGKCRSAGIKVIMVTGDHPITAKAIAKGVGI
  ISEGNETVEDIAARLNIPVSQVNPREAKACVVHGSDLKDMTSEQLDEILRDHTEIVFARTSPQQKLIIVE
  GCQRQGAIVAVTGDGVNDSPALKKADIGIAMGISGSDVSKQAADMILLDDNFASIVTGVEEGRLIFDNLK
  KSIAYTLTSNIPEITPFLLFIIANIPLPLGTVTILCIDLGTDMVPAISLAYEAAESDIMKRQPRNSQTDK
  LVNERLISMAYGQIGMIQALGGFFTYFVILAENGFLPSRLLGIRLDWDDRTTNDLEDSYGQEWTYEQRKV
  VEFTCHTAFFASIVVVQWADLIICKTRRNSVFQQGMKNKILIFGLLEETALAAFLSYCPGMGVALRMYPL
  KVTWWFCAFPYSLLIFIYDEVRKLILRRYPGGWVEKETYY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012505
• ORF Size: 3060 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_012505.1, NM_012505.2, NP_036637.1
• RefSeq Size: 5149
• RefSeq ORF: 3063
• Locus ID: 24212
• MW: 112.2 kDa
• Gene Summary: catalytic subunit of the membrane ATPase; involved in ATP dependent transport of Na+ and K+; necessary for creating and maintaining electrochemical gradient across plasma membrane [RGD, Feb 2006]