Crkl (NM_001008284) Rat Tagged ORF Clone

CAT#: RR214888

Crkl (Myc-DDK-tagged ORF) - Rat v-crk sarcoma virus CT10 oncogene homolog (avian)-like (Crkl), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Crkl
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214888 representing NM_001008284
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCTCCGCCAGGTTTGATTCTTCAGACCGTTCTGCCTGGTACATGGGGCCAGTGTCTCGCCAGGAGG
  CGCAGACCCGTCTCCAGGGCCAGCGCCATGGCATGTTCCTAGTCCGGGACTCATCTACCTGCCCTGGGGA
  CTATGTACTGTCCGTGTCCGAGAACTCGCGTGTCTCGCACTACATCATCAACTCCCTGCCCAACCGCCGC
  TTTAAGATCGGGGACCAGGAGTTTGACCATTTGCCGGCCTTGCTGGAGTTCTACAAGATCCACTACCTGG
  ATACTACCACCTTAATCGAACCAGCGCCCAGGTACCCCAACCCACCAATGGGTTCTGTCTCAGCACCCAA
  CTTATCTACAGCAGAAGAAAATCTGGAATATGTACGGACTCTGTATGATTTTCCTGGGAATGATGCTGAA
  GACCTACCCTTTAAAAAGGGTGAGCTTCTAGTGATAATAGAAAAGCCTGAAGAACAGTGGTGGAGTGCCC
  GCAACAAGGACGGCCGGGTTGGGATGATTCCTGTCCCTTACGTTGAAAAGCTTGTGAGGTCCTCACCACA
  TGGAAAGCATGGAAATAGGAATTCTAACAGTTATGGCATCCCAGAACCTGCTCACGCATATGCTCAACCT
  CAGACCACAACTCCTCTACCTACAGTTGCCAGTACTCCTGGGGCAGCGATCAACCCTTTGCCATCCACAC
  AGAATGGACCTGTCTTTGCAAAAGCAATCCAGAAGAGAGTACCTTGTGCTTATGACAAGACTGCCTTGGC
  ATTGGAGGTTGGTGACATTGTGAAAGTCACAAGGATGAATATCAATGGCCAGTGGGAAGGCGAGGTGAAT
  GGGCGCAAGGGGCTTTTCCCCTTCACACATGTTAAAATCTTTGACCCTCAGAACCCCGATGATAACGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214888 representing NM_001008284
  Red=Cloning site Green=Tags(s)
  
  MSSARFDSSDRSAWYMGPVSRQEAQTRLQGQRHGMFLVRDSSTCPGDYVLSVSENSRVSHYIINSLPNRR
  FKIGDQEFDHLPALLEFYKIHYLDTTTLIEPAPRYPNPPMGSVSAPNLSTAEENLEYVRTLYDFPGNDAE
  DLPFKKGELLVIIEKPEEQWWSARNKDGRVGMIPVPYVEKLVRSSPHGKHGNRNSNSYGIPEPAHAYAQP
  QTTTPLPTVASTPGAAINPLPSTQNGPVFAKAIQKRVPCAYDKTALALEVGDIVKVTRMNINGQWEGEVN
  GRKGLFPFTHVKIFDPQNPDDNE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001008284
• ORF Size: 909 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001008284.1, NP_001008285.1
• RefSeq Size: 2803 bp
• RefSeq ORF: 912 bp
• Locus ID: 287942
• Cytogenetics: 11q23
• MW: 33.9 kDa