Pfkfb1 (NM_001271063) Rat Tagged ORF Clone

CAT#: RR216743

Pfkfb1 (myc-DDK-tagged) - Rat 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (Pfkfb1), transcript variant 2

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pfkfb1
• Synonyms: Pfkfb01; PFRX
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR216743 representing NM_001271063
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCACCCACCGGACCTGCTTTGGGGGTCTGTAAATGCAAGAGAGCCATTGGAAAATTAGCGATGGAAG
  AAAAAGCCTCTAAGAGAACAGCCTCCATACCACAGTTCACTAATTCTCCCACGATGGTGATCATGGTGGG
  TTTACCAGCTCGAGGCAAGACCTACATCTCTACGAAGCTCACACGCTATCTCAACTGGATAGGAACACCA
  ACTAAAGTGTTTAATTTAGGTCAGTATCGACGAGAGGCAGTGAGTTACAGGAACTATGAATTCTTTCGCC
  CAGACAACACAGAGGCCCAGCTTATCAGGAAGCAGTGTGCTCTAGCAGCCCTAAAGGATGTCCATAAGTA
  TCTCAGCCGCGAGGAAGGTCATGTTGCGGTTTTTGATGCCACCAACACTACCAGAGAACGACGATCGTTG
  ATTCTACAGTTTGCTAAGGAACATGGTTATAAGGTCTTCTTTATTGAGTCTATTTGTAATGACCCCGAAA
  TCATTGCAGAAAACATCAAGCAAGTGAAACTTGGTAGTCCTGATTACATAGACTGTGACCAAGAAAAGGT
  TTTGGAAGACTTTCTAAAGAGAATAGAGTGCTATGAGATCAACTACCAACCTTTGGATGAGGAATTGGAC
  AGCCACCTGTCCTACATCAAGATCTTCGACGTGGGCACACGCTACATGGTAAATCGAGTGCAGGACCACG
  TTCAGAGCCGTACAGCCTACTACCTCATGAACATCCATGTCACACCTCGATCTATCTACCTATGCCGCCA
  TGGTGAGAGTGAACTCAACCTTAGAGGCCGCATTGGAGGTGACTCTGGCCTCTCAGCTCGGGGCAAGCAG
  TATGCCTATGCACTAGCCAACTTCATCCGGTCTCAAGGCATCAGCTCCCTGAAAGTATGGACTAGCCACA
  TGAAGAGGACCATTCAGACCGCTGAAGCCCTAGGTGTCCCCTATGAACAGTGGAAGGCCCTGAATGAGAT
  TGATGCGGGTGTCTGTGAAGAGATGACCTATGAAGAAATTCAGGAACACTACCCTGAGGAATTTGCACTA
  CGGGACCAGGATAAATATCGTTACCGCTATCCCAAGGGAGAGTCCTATGAGGATCTGGTTCAGCGTCTTG
  AACCAGTTATAATGGAGCTAGAACGGCAAGAAAATGTACTGGTGATCTGTCACCAGGCTGTCATGCGGTG
  CCTCCTGGCATACTTCCTGGATAAAAGTTCAGATGAGCTGCCCTATCTCAAGTGTCCTCTGCATACTGTG
  CTCAAACTCACACCTGTGGCTTATGGCTGCAGAGTGGAGTCCATCTACCTGAATGTGGAGGCTGTGAACA
  CACACCGGGACAAGCCTGAGAATGTGGACATCACCCGTGAAGCTGAGGAAGCCTTGGACACTGTACCTGC
  CCATTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR216743 representing NM_001271063
  Red=Cloning site Green=Tags(s)
  
  MPPTGPALGVCKCKRAIGKLAMEEKASKRTASIPQFTNSPTMVIMVGLPARGKTYISTKLTRYLNWIGTP
  TKVFNLGQYRREAVSYRNYEFFRPDNTEAQLIRKQCALAALKDVHKYLSREEGHVAVFDATNTTRERRSL
  ILQFAKEHGYKVFFIESICNDPEIIAENIKQVKLGSPDYIDCDQEKVLEDFLKRIECYEINYQPLDEELD
  SHLSYIKIFDVGTRYMVNRVQDHVQSRTAYYLMNIHVTPRSIYLCRHGESELNLRGRIGGDSGLSARGKQ
  YAYALANFIRSQGISSLKVWTSHMKRTIQTAEALGVPYEQWKALNEIDAGVCEEMTYEEIQEHYPEEFAL
  RDQDKYRYRYPKGESYEDLVQRLEPVIMELERQENVLVICHQAVMRCLLAYFLDKSSDELPYLKCPLHTV
  LKLTPVAYGCRVESIYLNVEAVNTHRDKPENVDITREAEEALDTVPAHY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001271063
• ORF Size: 1407 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001271063.1, NP_001257992.1
• RefSeq Size: 1707
• RefSeq ORF: 1410
• Locus ID: 24638
• MW: 54.2 kDa
• Gene Summary: bifunctional enzyme; catalyzes synthesis and degradation of fructose 2,6-bisphosphate, a ubiquitous stimulator of glycolysis [RGD, Feb 2006]