Stefin B (CSTB) (NM_000100) Human Untagged Clone

CAT#: SC100002

CSTB (untagged)-Human cystatin B (stefin B) (CSTB)


  "NM_000100" in other vectors (6)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol CSTB
Synonyms CPI-B; CST6; EPM1; EPM1A; PME; STFB; ULD
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC100002 sequence for NM_000100 edited (data generated by NextGen Sequencing)
ATGATGTGCGGGGCGCCCTCCGCCACGCAGCCGGCCACCGCCGAGACCCAGCACATCGCC
GACCAGGTGAGGTCCCAGCTTGAAGAGAAAGAAAACAAGAAGTTCCCTGTGTTTAAGGCC
GTGTCATTCAAGAGCCAGGTGGTCGCGGGGACAAACTACTTCATCAAGGTGCACGTCGGC
GACGAGGACTTCGTACACCTGCGAGTGTTCCAATCTCTCCCTCATGAAAACAAGCCCTTG
ACCTTATCTAACTACCAGACCAACAAAGCCAAGCATGATGAGCTGACCTATTTCTGA

Clone variation with respect to NM_000100.2
>OriGene 5' read for NM_000100 unedited
GCACATTTTGTAATACGACTCACTATAGGGGCGGCCGCGATTCGGCACGAGGCCTCGTGC
CGAATTCGGCACGAGGGTCGCCGCCAAGATGATGTGCGGGGCGCCCTCCGCCACGCAGCC
GGCCACCGCCGAGACCCAGCACATCGCCGACCAGGTGAGGTCCCAGCTTGAAGAGAAAGA
AAACAAGAAGTTCCCTGTGTTTAAGGCCGTGTCATTCAAGAGCCAGGTGGTCGCGGGGAC
AAACTACTTCATCAAGGTGCACGTCGGCGACGAGGACTTCGTACACCTGCGAGTGTTCCA
ATCTCTCCCTCATGAAAACAAGCCCTTGACCTTATCTAACTACCAGACCAACAAAGCCAA
GCATGATGAGCTGACCTATTTCTGATCCTGACTTTGGACAAGGCCCTTCAGCCAGAAGAC
TGACAAAGTCATCCTCCGTCTACCAGAGCGTGCACTTGTGATCCTAAAATAAGCTTCATC
TCCGGGCTGTGCCCCTTGGGGTGGAAGGGGCAGGATTCTGCAGCTGCTTTTGCATTTCTC
TTCCTAAATTTCATTGTGTTGATTTCTTTCCTTCCCAATAGGTGATCTTAATTACTTTCA
GAATATTTTCAAAATAGATATATTTTTAAAATCCTTANNAAAAAAAAAAAANAAAAAAAA
AAAAAANNAAAAAAAAAAAAAACCTCGACTCTAGATTGCGGCCGCGGTCATAGCTGTTTC
CTGAACAGATCCCGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGNA
AGTTGCCACTCCAGTGCCCACCAGCCTTGTCCTAATAAAATTAAGTTGCATCATTTTGTC
TGACTAGGTGTCCTTCTATAATATATGGGGTGGAGGNGGGTTGNAATGGACCAAGGGCCA
GTTTGGAAAACACCTGTAGGGCCGCGGGGCCTATGGGAACCAGCTGAAT
>OriGene 3' read for NM_000100 unedited
GGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
TTTTTTTAAGGATTTTAAAAATATATTTATTTTGAAAATATTCTGAAAGTAATTAAAATC
ACCTTTTGGGAAGGAAAGAAATCAACCCAATGAAATTTAGGAAAAAAAATGCAAAAGCAG
CTGCAAAATCCTGCCCCTTCCACCCCAAGGGGCACAGCCCGGAAATGAAGCTTATTTTAG
GATCACAAGTGCCCCCTCTGGTAAACGGAGGATGACTTTGTCAGTCTTCTGGCTGAAGGG
CCTTGTCCAAAGTCAGGATCAAAAATAGGGCAGCTCATCATGCTTGGCTTTGTTGGTCTG
GTAATTAAATAAGGGCAAGGGCTTGTTTTCATGAGGGAAAAATTGGAACACTCGCAGGTG
TACAAAGTCCTCGTCGCCGACGTGCACCTTGATGAAGTAGTTTGTCCCCGCGACCACCTG
GCTCTTGAATGACACGGCCTTAAACACAGGGAACTTCTTGTTTTCTTTCTCTTCAAGCTG
GGACCTCACCTGGTCGGCGATGTGCTGGGTCTCGGCGGTGGCCGGCTGCGTGGCGGAGGG
CCCCCCGCACATCATCTTGGCGGCGACCCTCGTGCCGAATTCGGCACGAGGCCCTCGTGC
CGAATTCGCGGCCGCCCTATAGTGAGTCGTATACAAAATTCTGACGGTTCACTAAACGAG
CTCTGCTTATATAGACCTCCCACCGTACACGCCTACCGNCCATTTGCGTCACGGGGCGGN
GTTATTACGACTTTTGAAAAGTCCGTTGATTTTGTGCCAAACAACTCCNATGACGTCATG
GGGTGGAAACTGAAATCCCGGATCAACCGTATCCGCCATTGTGTCTGCAAACGATCACTG
TATAGCAGACTATACCAAGTCTGCANTAGAAATCGTAGGCATTCTGGCTATGCAGCGGCC
TTCC
Restriction Sites NotI-NotI     
ACCN NM_000100
Insert Size 640 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000100.2, NP_000091.1
RefSeq Size 674 bp
RefSeq ORF 297 bp
Locus ID 1476
Cytogenetics 21q22.3
Domains CY
Gene Summary 'The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and kininogens. This gene encodes a stefin that functions as an intracellular thiol protease inhibitor. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. The protein is thought to play a role in protecting against the proteases leaking from lysosomes. Evidence indicates that mutations in this gene are responsible for the primary defects in patients with progressive myoclonic epilepsy (EPM1). One type of mutation responsible for EPM1 is the expansion in the promoter region of this gene of a CCCCGCCCCGCG repeat from 2-3 copies to 30-78 copies. [provided by RefSeq, Jul 2016]'

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