MARCH8 (NM_145021) Human Untagged Clone

CAT#: SC100050

41341 (untagged)-Human membrane-associated ring finger (C3HC4) 8 (MARCH8), transcript variant 8

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MARCH8
• Synonyms: c-MIR; CMIR; MARCH-VIII; MIR; RNF178
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_145021, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGCATGCCACTGCATCAGATCTCTGCCATTCCATCCCAGGATGCCATCTCTGCTAGAGTCTACAGAA
  GTAAGACCAAAGAAAAGGAGAGGGAAGAACAGAATGAGAAGACTTTGGGACATTTCATGAGTCATTCAAG
  CAACATTTCTAAGGCTGGGAGTCCTCCGTCAGCATCAGCTCCGGCTCCGGTGTCCTCCTTCTCTCGCACT
  TCTATCACGCCATCCAGCCAGGACATCTGCAGGATCTGCCACTGTGAAGGAGATGATGAGAGCCCCCTGA
  TCACCCCCTGCCACTGCACAGGAAGCCTCCACTTCGTGCACCAGGCCTGCCTGCAGCAGTGGATCAAGAG
  CTCCGACACGCGCTGCTGCGAGCTCTGCAAGTATGAGTTCATCATGGAGACCAAGCTGAAGCCACTGAGA
  AAATGGGAGAAGTTGCAGATGACGTCCAGCGAGCGCAGGAAGATCATGTGCTCAGTGACATTCCACGTCA
  TTGCCATCACATGTGTGGTCTGGTCCTTGTATGTGCTCATTGACCGTACTGCTGAGGAGATCAAGCAGGG
  GCAGGCAACAGGAATCCTAGAATGGCCCTTTTGGACTAAATTGGTGGTTGTGGCCATCGGCTTCACCGGA
  GGACTTCTTTTTATGTATGTTCAGTGTAAAGTGTATGTGCAATTGTGGAAGAGACTCAAGGCCTATAATA
  GAGTGATCTATGTTCAAAACTGTCCAGAAACAAGCAAAAAGAATATTTTTGAAAAATCTCCACTAACAGA
  GCCCAACTTTGAAAATAAACATGGATATGGAATCTGTCATTCCGACACAAACTCTTCTTGTTGCACAGAG
  CCTGAAGACACTGGAGCAGAAATCATTCACGTCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_145021 unedited
  NGGGTTATTACCCCCGCCCGTTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTA
  TATAAGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGC
  GGCCGCGAATTCGGCACGAGGGTTGGGATGAGCATGCCACTGCATCAGATCTCTGCCATT
  CCATCCCAGGATGCCATCTCTGCTAGAGTCTACAGAAGTAAGACCAAAGAAAAGGAGAGG
  GAAGAACAGAATGAGAAGACTTTGGGACATTTCATGAGTCATTCAAGCAACATTTCTAAG
  GCTGGGAGTCCTCCGTCAGCATCAGCTCCGGCTCCGGTGTCCTCCTTCTCTCGCACTTCT
  ATCACGCCATCCAGCCAGGACATCTGCAGGATCTGCCACTGTGAAGGAGATGATGAGAGC
  CCCCTGATCACCCCCTGCCACTGCACAGGAAGCCTCCACTTCGTGCACCAGGCCTGCCTG
  CAGCAGTGGATCAAGAGCTCCGACACGCGCTGCTGCGAGCTCTGCAAGTATGAGTTCATC
  ATGGAGACCAAGCTGAAGCCACTGAGAAAATGGGAGAAGTTGCAGATGACGTCCAGCGAG
  CGCAGGAAGATCATGTGCTCAGTGACATTCCACGTCATTGCCATCACATGTGTGGTCTGG
  TCCTTGTATGTGCTCATTGACCGTACTGCTGAGGAGATCAAGCAGGGGCAGGCAACAGGA
  ATCCTATAAGGGCCCTTTGGACAAATTGGTGGGTGTGGCCATCGGCTTCACCGGAGGACT
  TCTTTTTATGTATGTTCACTGCAAAGCGCATGTGCCATTGTCGGAAAAGACCCAGGCCCT
  TTATAGAAAGGACTATTGTTCAAAACCGTCCCGAAACAACCAAAAAGAATATTTTTCGAA
  AAACTCCACCAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_145021 unedited
  TCNAACGTAATCTATGNACGCGGCCGCATTCTANGATCGGTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCTTTTTAAAAAAAGGCTTTATT
  GGTGCAATTAAACATCCCAGGGTATAGCACAAACCCCCCTGTACAAAAAATTATTCAAGG
  GGTAATACTGAAAAACAGTGAACCCCCCCAAAAAAATACAAAACTAGCCATTTAGATCAC
  TAAAAACTTTTTAAGGGAAAAAAAATTTCAAATGGGAAGGGCCTTTTAAAAACTACCCCC
  CACATGCCAGGGGAAATTTGGTTTAACAATCAATTTTTATAAACATTGGATTTAACTGGG
  CACTGATAAACTGGACAATTTTAAAAATTACTTCCAAGACAAAAATAAATGCTTTTATTT
  CCCTATGTTTTGGGCTTTCCTTGGGCTAAAAACAAAAGCTGCATGTTTCAAACCCAATTT
  CAAAGGGAAAGCCCTTTAAAACAAAAAGGACTTCAATTGGGTTTTTGCCTTTAGCCACAT
  CCAAAGACTTGGGGATCTCACCCGGGTGGTCCCAACCCTTTTTCAACCCTGAAACCCAAG
  CAGGGGGGCAAAGGCCCCCCCCAATTGTGCAAACATTCACCGAGGTTCCAAAATGGCCCA
  ACTGGCTTGGGCCTGTTTGCCTGTGGGCCAGTTAGGAACAATGTGCCGGTTTGAAAAACA
  AGGCCCCCCAAACTGGAAAAGTGGCGCGAAACCACAAGGGGGGAAAAAATTTGAGGCGCT
  CACGGGGGGAAAGAACCCTTTTATAGAGTTTTCCGGAAAATCTTACCAAAAAATTGGGCC
  ACAAAACAAATTTTTGGAGAGCAAAAACCCTCTTTATGGTACCGGGGGATT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_145021
• ORF Size: 876 bp
• Insert Size: 4700
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_145021.4, NP_659458.2
• RefSeq Size: 2511
• RefSeq ORF: 876
• Locus ID: 220972
• Domains: RING
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: MARCH8 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH8 induces the internalization of several membrane glycoproteins (Goto et al., 2003 [PubMed 12582153]; Bartee et al., 2004 [PubMed 14722266]). [supplied by OMIM, Apr 2010]
  Transcript Variant: This variant (2) differs in the 5' UTR and lacks an in-frame exon in the 3' coding region, compared to variant 4. This results in a shorter protein (isoform a), compared to isoform b. Variants 2 and 3 encode the same isoform (a).