NEIL2 (NM_145043) Human Untagged Clone

CAT#: SC100057

NEIL2 (untagged)-Human nei endonuclease VIII-like 2 (E. coli) (NEIL2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NEIL2
• Synonyms: NEH2; NEI2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC100057 sequence for NM_145043 edited (data generated by NextGen Sequencing)
  ATGCCAGAAGGGCCGTTGGTGAGGAAATTTCACCATTTGGTCTCCCCCTTTGTGGGTCAG
  CAGGTGGTCAAGACAGGGGGCAGCAGTAAGAAGCTACAGCCCGCCAGCCTGCAGTCTCTG
  TGGCTCCAGGACACCCAGGTCCATGGAAAGAAATTATTCCTTAGATTTGATCTAGATGAA
  GAAATGGGGCCCCCTGGCAGCAGCCCAACACCAGAGCCTCCACAAAAAGAAGTGCAGAAG
  GAAGGGGCTGCGGACCCAAAGCAGGTCGGGGAGCCCAGCGGGCAGAAGACCCTTGATGGA
  TCCTCACGGTCTGCAGAGCTCGTCCCCCAGGGCGAGGATGATTCTGAGTATTTGGAGAGA
  GACGCCCCTGCAGGAGATGCTGGGAGGTGGCTGCGTGTCAGCTTTGGTTTGTTTGGCAGC
  GTTTGGGTGAACGATTTCTCCAGAGCCAAGAAAGCCAACAAGAGGGGGGACTGGAGGGAC
  CCTTCCCCGAGGTTGGTCCTGCACTTTGGTGGTGGTGGCTTCCTGGCATTTTATAATTGT
  CAGTTGTCTTGGAGCTCTTCCCCAGTGGTCACACCCACCTGTGACATCCTGTCTGAGAAG
  TTCCATCGAGGACAAGCCTTAGAAGCTCTAGGCCAGGCTCAGCCTGTCTGCTATACACTG
  CTGGACCAGAGATACTTCTCAGGGCTAGGGAACATCATTAAGAATGAAGCCTTGTACAGA
  GCTGGGATCCATCCCCTTTCTCTCGGTTCAGTCCTGAGTGCCTCGCGTCGGGAGGTCCTG
  GTGGATCACGTGGTGGAGTTCAGTACAGCCTGGCTGCAGGGCAAGTTCCAAGGCAGACCG
  CAGCACACACAGGTCTACCAGAAAGAACAGTGCCCTGCTGGCCACCAGGTCATGAAGGAG
  GCGTTTGGGCCCGAAGATGGGTTACAGAGGCTCACCTGGTGGTGCCCGCAGTGCCAGCCC
  CAGTTGTCAGAGGAGCCAGAGCAGTGCCAGTTCTCCTAA
  
  Clone variation with respect to NM_145043.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_145043 unedited
  TACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGAAGGGAGGGCGGGCCCCGGG
  CCCTCCTCCGTCTCAGCCGCCTGCGGAGGTGCTGCCCACGCCTGGAGGCCCCCACTGACC
  CTCAGACCCGCGTCTGCGCCCCTCTCCCCGCACCCCGAGGCAGAGTTGGGAAAGCAGTGG
  TCTTAGACCCCCCACCTCGGGCACTCGGAAGAGAACGGCGGAGACAACCCCTCCTCTTCC
  CTGGCTGGCGCAGCGCCAGCCTCGAGCTCCTCGGTAGCCCCCGGGCAGGGAGGGCCGGAG
  GGTGGGCGCGGCATCTTCAGCGACTCTTCGAAGTCCCTTCCGCGTCTCATCTTTCAAGGC
  TGTTGCAGAGGCGGCTTGCTTCCCACCTGTCCATCTCCATAAAAATCCCTAAACGAAACA
  TGCCCACGTGTCCGGAGATTTTCAGGACTTGGTGCATTTCAGATGAAGGCTTTTCCAGAA
  GCTTCCCCGTAGAAGAGGATCAGGCATCCAACTGGTTAAGGGATGCCAGAAGGGCCGTTG
  GTGAGGAAANTTCACCATTTGGTCTCCCCCTTTGTGGGTCAGCAGGTGGTCAAGACAGGG
  GGCAGCAGTAAGAAGCTACAGCCCGCCAGCCTGCAGTCTCTGTGGCTCCAGGACACCCAG
  GTCCATGGAAAGAAATATTCCTTAGATTTGATCTAGATGAAGAAATGGGGCCCCCTGGCA
  GCAGCCCAACACCAGAGCCTNCACANAAAGAAGTGCAGAAGGAAGGGGCTGCNGACCCAA
  TNGCAGTCGNGAGCCCAGCGGGCANNAGACCCCTGATGGATCCTCACGGTCTGCAGAGCT
  CGTCCCCAGGGCGAGGATGATNCTGAGTATTTGGAGAGAGACNCCCCTGCAGAATGCCTG
  GNAGTGGCTGCGTGCANCTTGGNTTGTTGGCACCGTTTGGTGAACCATTCTCGAGCCAGA
  AGCACAGGAGGGGGCTGGAGGCCTTCCCCCAGGTGGTCTGACTCTGGGGGGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_145043 unedited
  NNCGGTACGACTATGNNACCGCGGCCGCAANCTANGATCGAGTTTTTTTTTTTTTTTTTT
  TTTTTTGCGAGTAACAATGAGCTTTATTTTCATGTCACCGCTCTAGGTTCATTCACCTGT
  TGCCAACAACATGAGGGAGTTTCTCCACAGCCCTTGATTCCTGAATTGTTCAACAAATCA
  ACAGTCACCAATACATTGACTTAAGGAGTAAACAAGCAGCTTTTCTCTACACACCACCGG
  GCACCCCAGGAAGGCGCAGCTCCCTGCTCCACCCCCGCCTCTTGGTGCCTGTATCCAGCA
  GAAGGACCCAGCAGGAGGCTGAATCCAAGCACTGCTCTCAGGCCACTCTATCCTCATCCC
  TGGATCCTCCAGGGAACAAGATAAAAAAAAAAAAATCCTGTTTTCCTGCTTTCCAAAATG
  GAGGGCCTTAACATCAACCAAAGTTACCAAACAAAAACAAAACACAGGAGCTAGCTGGGC
  GCGGTGGCTCACACCCGTGGTCCCAACATTTTGGGAGACCAAGGTGGGAGGAATGCTTGA
  GTTGTAGGAGTTCAAACCAGCCTGGCAAATATGGCAAGACCCCATCTCTCTAAAAAGTAA
  ACAAAAATCAGCCGAGCATCATATCACATGCCTGTAGTCCCAGCTACTCTGGAGGCTGAG
  ATGAAGACTGCTTGAGCCAGGGAGGGCAAGGCTGCCGTGAGCCAAGATCACACCACCACA
  CCCTAGCCAGGGAGACAGAGCAAGACTCTCTCTCAAACAAACAACCCAAAAAAAAAAAAA
  AAAACCCACATCATCTGCAAAGCTATGTTGGGAGCACAGCCATTTCCCATAGGCTTTCTT
  TTTCCTTGCGCCATTGCCTTCCGGGAAGTTTTCCCCTTACCATCATCACGATTTTTCCAT
  CTTTCACGATGGTGCAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_145043
• ORF Size: 999 bp
• Insert Size: 2650
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_145043.1, NP_659480.1
• RefSeq Size: 2661
• RefSeq ORF: 999
• Locus ID: 252969
• Domains: Fapy_DNA_glyco
• Protein Families: Druggable Genome
• Protein Pathways: Base excision repair
• Gene Summary: This gene encodes a member of the Fpg/Nei family of DNA glycosylases. These glycosylases initiate the first step in base excision repair by cleaving oxidatively damaged bases and introducing a DNA strand break via their abasic site lyase activity. This enzyme is primarily associated with DNA repair during transcription and acts prefentially on cytosine-derived lesions, particularly 5-hydroxyuracil and 5-hydroxycytosine. It contains an N-terminal catalytic domain, a hinge region, and a C-terminal DNA-binding domain with helix-two-turn-helix and zinc finger motifs. This enzyme interacts with the X-ray cross complementing factor 1 scaffold protein as part of a multi-protein DNA repair complex. A pseudogene of this gene has been identified. [provided by RefSeq, Mar 2017]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Variants 1, 2, and 8 encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.