TTL (NM_153712) Human Untagged Clone

CAT#: SC100834

TTL (untagged)-Human tubulin tyrosine ligase (TTL)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TTL
• Synonyms: MGC46235
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_153712, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTACACCTTCGTGGTACGCGATGAGAACAGCAGCGTCTACGCCGAGGTCTCCCGGCTGCTCCTCGCCA
  CCGGCCACTGGAAGAGGCTGCGGCGAGACAACCCCAGATTCAACCTGATGCTGGGAGAGAGGAATCGGCT
  GCCCTTCGGGAGACTGGGTCACGAGCCCGGGCTGGTACAGTTGGTGAATTACTACAGGGGTGCTGACAAA
  CTGTGTCGCAAAGCTTCTTTAGTGAAGCTAATCAAGACAAGCCCTGAACTGGCTGAGTCCTGCACATGGT
  TCCCTGAATCTTATGTGATTTATCCAACCAATCTCAAGACTCCAGTTGCTCCAGCACAGAATGGAATTCA
  GCCACCAATCAGTAACTCAAGGACAGATGAAAGAGAATTCTTTCTCGCCTCTTATAACAGAAAGAAAGAG
  GATGGAGAGGGCAACGTTTGGATTGCAAAGTCATCAGCCGGTGCCAAAGGTGAAGGCATTCTCATCTCCT
  CAGAGGCTTCAGAGCTTCTCGATTTCATAGACAACCAGGGCCAAGTGCACGTGATCCAGAAATATCTTGA
  GCACCCTCTGCTGCTTGAGCCAGGTCATCGCAAGTTTGACATCCGAAGCTGGGTCTTGGTGGATCATCAG
  TATAATATCTACCTCTATAGAGAGGGTGTGCTTCGGACTGCTTCAGAACCATATCATGTTGATAATTTCC
  AAGACAAAACCTGCCATTTGACCAATCACTGCATTCAAAAAGAGTATTCAAAGAACTACGGGAAGTATGA
  AGAAGGAAATGAAATGTTCTTCAAGGAGTTCAATCAGTACCTAACAAGTGCTTTGAACATTACCCTAGAA
  AGTAGTATCTTACTACAAATCAAACATATAATAAGGAACTGCCTCCTGAGCGTGGAGCCTGCCATTAGCA
  CCAAGCACCTCCCTTACCAGAGCTTCCAGCTCTTCGGCTTTGACTTCATGGTCGATGAGGAGCTGAAGGT
  GTGGCTCATTGAGGTCAACGGTGCCCCTGCATGTGCTCAGAAGCTCTATGCAGAACTGTGCCAAGGCATC
  GTGGACATAGCCATTTCCAGTGTCTTCCCACCCCCAGATGTGGAGCAACCTCAGACCCAGCCAGCTGCCT
  TCATCAAGCTGTGA
  
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_153712 unedited
  TTTTTANNCGGTAAATCTAAGGACCGCGGNCCGCATNCTANGATCGAGTTTTTTTTTTTT
  TTTTTTGAAAATCATTACTTTACTTTAGTGTTCCAGGCATTTTAAATATTCATACAATCG
  CCTTATTTCAGCAGTCATTTCCTCTCCAGTACAGATTTTGTGTCCAGAACCAGAGCCATG
  TACGTCACTGAATTTTCTCTACAGCACCCGCATGCTACACGGGACCTACTCCGGTGCCCA
  TGTCTTTGCTAGTAGAGCCTCTCACCACACTTGCAAGAGGGCTGGACCTCCCCTTGCTTG
  ATGTAGCCTCCACTGTGAATGAAAACACACTTTGTCACTTCGATCCCAGTACATTTCCAG
  CACTCCCAGGTGGTCCATGGATGTGGCAATCATCCCACAGAGCTAGTGACACATCATGGC
  AGCTGGGACCTATGTCAACACCTGGTCCACTATACATACCGATGCCGAAGCACTTAAATG
  GTCCTGGTAAAATGGAATTTATAATTGCCATAAGGATTGAATTTTGGGGAAAAGGGACTT
  TCAATAATTAATACCCCTAATACCTATTTAGCAGAATTAAATTTTCGTGTCTGTTTACAT
  TTGGTGTCCAAGCTTCCTAAACTCATTATAGGTAATGAAAAACTGTTTGAGTTTTCCAAA
  TGCTGCTTTGACAAGCAAACCGAAGAAACTGGTTTAACAACCCCCTGCCTTGGGGCAATA
  AAACCTTCGGGAACCTTCCGTTCATCCTTAAAGAAAGGCCTGGGGTTTCCAAAAAAAATT
  TTTTTTTTTAAATCCTAAGCCCTTAAGCCATTCTCCCAGATAATATGGGGTTTGTCCCTC
  CCTTCCCAGCAGGGGTCAAGGCATCTCAAGGCATATGCTGCCTGAGGTTA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_153712
• ORF Size: 5181 bp
• Insert Size: 4700
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info

Reference Data

• RefSeq: NM_153712.3, NP_714923.1
• RefSeq Size: 5181
• RefSeq ORF: 5181
• Locus ID: 150465
• Domains: TTL
• Gene Summary: TTL is a cytosolic enzyme involved in the posttranslational modification of alpha-tubulin (see MIM 602529). Alpha-tubulin within assembled microtubules is detyrosinated over time at the C terminus. After microtubule disassembly, TTL restores the tyrosine residues and consequently participates in a cycle of tubulin detyrosination and tyrosination (Erck et al., 2003 [PubMed 14571137]). [supplied by OMIM, Mar 2008]