DAGLA (NM_006133) Human Untagged Clone

CAT#: SC101029

DAGLA (untagged)-Human diacylglycerol lipase, alpha (DAGLA)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DAGLA
• Synonyms: C11orf11; DAGL(ALPHA); DAGLALPHA; NSDDR
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_006133 edited
  ATCGGGGCCTTGGGGAGCCCAGGATGGAGGTGGCGGTCGCGGCGGCGGGCCGAGCCCTGC
  GGCGGGCGGGAGGAGGTGAGCACCAGGCCCACTGAGCCTCTGCAGAGCCACCAGCCATGC
  CCGGGATCGTGGTGTTCCGGCGGCGCTGGTCTGTGGGCAGTGATGACCTCGTCCTACCAG
  CCATCTTCCTCTTTCTCCTGCATACCACCTGGTTTGTGATCCTGTCCGTGGTGCTCTTCG
  GCCTGGTCTATAACCCGCACGAGGCCTGCTCCCTGAACCTGGTGGACCACGGCCGCGGCT
  ACCTGGGCATCCTGCTGAGCTGCATGATCGCTGAGATGGCCATCATCTGGCTGAGCATGC
  GCGGGGGCATCCTCTACACGGAGCCCCGTGACTCCATGCAGTACGTGCTCTACGTGCGCC
  TGGCCATCCTGGTGATCGAGTTCATCTACGCCATCGTGGGCATCGTCTGGCTCACTCAGT
  ACTACACCTCCTGCAACGACCTCACTGCCAAGAATGTCACCCTCGGAATGGTTGTCTGCA
  ACTGGGTAGTCATCCTCAGTGTGTGCATCACTGTCCTCTGCGTCTTCGACCCCACGGGCC
  GCACCTTTGTCAAGCTGAGAGCCACCAAGAGGAGGCAGCGTAACCTGCGGACCTACAACC
  TGCGGCACCGCTTAGAGGAGGGTCAAGCCACCAGCTGGTCGCGCCGGCTCAAAGTGTTCC
  TCTGCTGCACGCGGACGAAGGACTCCCAGTCAGATGCCTACTCAGAAATCGCCTACCTCT
  TTGCGGAGTTCTTCCGGGACCTTGACATTGTGCCATCCGACATCATTGCTGGCCTGGTGC
  TGCTCCGGCAGCGGCAGCGGGCCAAGCGCAACGCCGTGCTGGACGAGGCAAACAATGACA
  TCTTGGCCTTCCTGTCTGGGATGCCGGTGACCAGAAACACCAAGTACCTCGACCTCAAGA
  ATTCACAAGAGATGCTCCGCTACAAAGAGGTCTGCTACTACATGCTCTTTGCCCTGGCTG
  CCTACGGGTGGCCCATGTACCTGATGCGGAAGCCCGCCTGCGGCCTCTGCCAACTGGCTC
  GGTCCTGCTCGTGTTGCCTGTGTCCTGCGAGGCCGCGGTTCGCCCCTGGAGTCACCATCG
  AGGAAGACAACTGCTGTGGCTGTAATGCCATTGCCATCCGGCGCCACTTCCTGGACGAGA
  ACATGACTGCGGTGGACATCGTCTATACCTCCTGCCATGATGCGGTCTATGAAACGCCCT
  TCTACGTGGCGGTGGACCATGACAAGAAGAAAGTGGTGATCAGTATCCGGGGGACCCTGT
  CCCCCAAGGATGCCCTGACTGACCTGACGGGTGATGCTGAGCGCCTCCCCGTGGAGGGGC
  ACCACGGCACCTGGCTGGGCCACAAGGGTATGGTCCTCTCAGCTGAGTACATCAAGAAGA
  AACTGGAGCAGGAGATGGTCCTGTCCCAGGCCTTTGGGCGAGACCTGGGCCGCGGAACCA
  AACACTACGGCCTGATTGTGGTGGGCCACTCCCTGGGCGCGGGCACTGCTGCCATCCTCT
  CCTTCCTTCTGCGCCCACAGTATCCGACCCTCAAGTGCTTTGCCTACTCCCCGCCAGGGG
  GCCTGCTGAGTGAGGATGCAATGGAGTATTCCAAGGAGTTCGTGACTGCTGTGGTTCTGG
  GCAAAGACCTCGTCCCCAGGATTGGCCTCTCTCAGCTGGAAGGCTTCCGCAGACAGCTCC
  TGGATGTCCTGCAGCGAAGCACCAAGCCCAAATGGCGGATCATCGTGGGGGCCACCAAAT
  GCATCCCCAAGTCGGAGCTGCCTGAGGAGGTAGAGGTGACCACCCTGGCCAGCACGCGGC
  TCTGGACCCACCCCAGCGACCTAACTATAGCCCTCTCAGCCAGCACTCCACTCTACCCGC
  CCGGCCGCATCATCCACGTGGTCCACAACCACCCTGCAGAGCAGTGCTGCTGCTGTGAGC
  AGGAGGAGCCCACATACTTTGCCATCTGGGGCGACAACAAGGCCTTCAATGAGGTGATCA
  TCTCGCCAGCCATGCTGCATGAGCACCTGCCCTATGTGGTCATGGAGGGGCTCAACAAGG
  TGCTGGAGAACTACAACAAGGGGAAGACCGCTCTGCTCTCTGCAGCCAAGGTCATGGTGA
  GCCCTACCGAGGTGGACCTGACTCCTGAGCTCATCTTCCAGCAGCAGCCACTCCCCACGG
  GGCCGCCCATGCCCACTGGCCTTGCCCTGGAGCTGCCGACTGCAGACCACCGCAACAGCA
  GCGTCAGGAGCAAGTCCCAGTCTGAGATGAGCCTGGAGGGCTTCTCGGAGGGGCGGCTGC
  TGTCGCCAGTGGTTGCGGCGGCGGCCCGCCAGGACCCGGTGGAGCTGCTGCTGCTGTCTA
  CCCAGGAGCGGCTGGCGGCGGAGCTGCAGGCCCGGCGGGCACCACTGGCCACCATGGAGA
  GCCTCTCGGACACTGAGTCCCTGTACAGCTTCGACTCGCGCCGCTCCTCAGGCTTCCGCA
  GCATCCGGGGCTCCCCCAGCCTCCACGCTGTGCTGGAGCGTGATGAAGGCCACCTCTTCT
  ACATTGACCCTGCCATCCCCGAGGAAAACCCATCCCTGAGCTCGCGCACTGAGCTGCTGG
  CGGCCGACAGCCTGTCCAAGCACTCACAGGACACGCAGCCCCTGGAGGCGGCCCTGGGCA
  GTGGCGGCGTCACTCCTGAGCGGCCCCCCAGTGCTGCGGCCAATGACGAGGAGGAAGAGG
  TTGGCGGTGGGGGTGGCGGGCCGGCCTCCCGCGGGGAGCTGGCGCTGCACAATGGGCGCC
  TGGGGGACTCGCCCAGTCCTCAGGTGCTGGAATTCGCCGAGTTCATCGACAGCCTCTTCA
  ACCTGGACAGCAAGAGCAGCTCCTTCCAAGACCTCTACTGCATGGTGGTGCCCGAGAGCC
  CCACCAGTGACTACGCTGAGGGCCCCAAGTCCCCCAGCCAGCAAGAGATCCTGCTCCGTG
  CCCAGTTCGAGCCCAACCTGGTGCCCAAGCCCCCACGGCTCTTTGCCGGCTCAGCCGACC
  CCTCCTCGGGCATCTCACTCTCGCCCTCCTTCCCGCTCAGCTCCTCGGGTGAGCTCATGG
  ACCTGACGCCCACGGGCCTCAGTAGCCAGGAATGCCTGGCGGCTGACAAGATCCGGACTT
  CTACCCCCACTGGCCACGGAGCCAGCCCCGCCAAGCAAGATGAGCTGGTCATCTCAGCAC
  GCTAGCACCCCAGTTGCGTGGCCAGCCGGGCCCAGGCAGGAGCAGGTGGCCCTGTGGGCA
  CCTGGTGCCTGCCCCCTGCCGGGCAGCTTTAAGGACAGACCCCCAGGGGCAGTTTAGCCT
  CAGGCACAGGCATCGCTGCTGAGCTGGGGGTCCGCATCCCTACCTCAGCTTAGGACCCCC
  AGAGCCAAGGTGGCTGGGATCTGGCCCCACAGATGGGGAAAGATGGGGAAGGGTGTGGAG
  TGGGGAGGAGCCTGGGCAGCCTGCTGGGTGGGCCACACTCAGCCTGACTGCCCTCCATGG
  GGGCATTCTGGCACCCCCTGCTCCAGGACAGGCCATGGGCAAGCTGCCTCCCATCACTGC
  CTGCTGGCTGCTCTCCCAGGGGCCAGGTGGAGAGCAGTGCCCCCCGACACATGTATTCTC
  ATCTGTGGTCCAGGCCGGCATCGTCCTGGCCACCCCCCAGATCTGGTGCCTGCTGGCCGG
  CCCCCTGGGGTGCCCCTGCCGAGGTGGCCTGCAGTGCTGTACATGTTTACAGAAGCTGCT
  GGGCTTGGCTCAGGATGTGTTCTGGGCTTGCAAGCCCCCCGCCCAATCATGTGTTCAGTA
  GCCGTCCTCTGAGCAGGGCCCAAGGCAGCCAGGGGCCTGGAGGGGCCAGAGGAGGGTGGG
  GTCAGGGCCGCCCCTTCTCTGCCTTGTGCCTCTCATGCTGCCTCCTCTGCCCATGGGTCC
  TGGGCACCCAGGCCTGCCCTGCCTGCTGGCTACTTCCTGGCTTACCTTCTACCCCCAAGG
  ATCCTCACCACCCAAAGGGTGGTGGGCACTGCTGTGACCACCCCAGCTGCAGAGTCAGTG
  CCCTGGGTGGAAGGAAGGCACTGAGAGCCCCCTTCCTCTGAGGGCCCCACCTCACCCCTT
  GGTGTCACCCCCACCACGCCTAGGCAGCTCTGGGCCCTGGGATCTGGAACCAACACACCC
  CTGTTCCCCTCAGCTTTCCCTCCTCGCTGGCCTGGGCACCCTCCTGGGAGCAGGCCTTCC
  TCCCTCCCACCCCCAATGTCCTGTTGGTAGGAGGTGGGGCCAAGAGTGGGGTATGGTGGG
  CCTTGGCTGGAGACCTCTGTCCACTGCCCAGGGAGGGGCCTGGGGCTGGGAGCAGTCCCG
  GTTTAGCCTGAGGTCCCCATAGGGCTTCCTCCCCTGCTGGGTTTGGGAAGCAGTTAGGGA
  GATAGCGACCCGGAGTTTCCCCAGAAGCGGGGTGGGAGGGTGTGCATGCTAGTGTTGGCG
  CGTATGCATGTGCATGAGTGTGCACCGTTCCTAAGGAAGGGGCCTCTGGGGCTGCCCACC
  CTACCTGCCCTGCCTGCCTGCTGCCCCTCCCAGCCTGCCAAGAAAACGGTAGGGGAGCAT
  GATGGGGCCTTTGAGGCAGGGTCGCAGGGACAAGCTCAGCTTTAGGCACCATCTGTTCCC
  ATCACGCCTGCTGCTGTGACCCGTTTTGGAAAACTGGTGTGTACCGAGGCGCTGACTGCA
  CGGCTGACCGCCTGCTCGTGCCTTCATTCTGCAGCGGCATGGTCCCTCCCATTCTGGCTC
  CACCTGCAGCCTCCCTGGGTGGCCTAGGCTCCCCCGACCAAGAGACCTCCCTCTCATGAT
  CACTGGTACCTGGGGGCCTGAATTCTGGCCCCCGGCTCCCCACACAGCTGGGACTGGCCT
  GGATGGCTGTCCTGGGAGCCCCTGCCCACCCTGACGGAGCTGGGCCTCCCCTCATCCTCT
  GTAACTCCCGCCTTCACCAGACTCAAGGACACCCTGGCCCTGCTGAGGCATACAGAGCTT
  CAGCCCAGCACAGAAGCAAGACAAAATCAGTGGCTCTTAGAGTTTAGAAAACAAGACAGA
  CTCTCAGATGAAAGATCTGACAAGCACCGTGGCCAGTCACAGGGAGAGACTTGATGTCTG
  GCCTTTTAATTCCTCCTCTGCCAGGGTGGGTCCTGGGACCTCTAATGTGGGCATGTCGTC
  CACCCCAGGACGAGCCATCAGGGACAGACCCCCCACCCCCAAGGCTGCAGCCACACCATG
  TTTCAGGCTTGGGGCTGGGGCAGGCTTGGGCTCAATCCTGGGCACCCAGGGGCAGCCCAC
  CCCTAACCTGGCTCCTACCCACCTTGCCCTTGAAGGATGGGCCTGCTGCACGTCTCCCTC
  CTCCACCCCATACCACACTGGGGGGTCTGAGCCACCCCCCTCAGCCCCGTTCGGCTCAGA
  CCGACCCCCACTCCATCCCCAGACCTGCAGCACAAGTGCGCGGGCCTGTCCTCCCAGGGG
  CCTGGGCGACTCCATATGCAATCAGTAGCGAGCAGCCGGGCCCCACAGACCCTCATGCAC
  TCTCTTACGTGCCATTCTCCCCAGACTTTTTTTGTACTTAATGTATGAAAGATCCAAACT
  AATATTGCTGTAAAAAGGAGAGACAAATTAATATAGCTTATTCTATAAATATATCTGTAT
  ATAAAGGTTTCTGTATATTGTATAGAGCTGTGTATAAACTGGATGTAGAAGCAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_006133
• Insert Size: 5800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006133.1, NP_006124.1
• RefSeq Size: 5756 bp
• RefSeq ORF: 3129 bp
• Locus ID: 747
• Cytogenetics: 11q12.2
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: 'This gene encodes a diacylglycerol lipase. The encoded enzyme is involved in the biosynthesis of the endocannabinoid 2-arachidonoyl-glycerol.[provided by RefSeq, Nov 2010]'