DAGLA (NM_006133) Human Untagged Clone

CAT#: SC309995

DAGLA (untagged)-Human diacylglycerol lipase, alpha (DAGLA)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DAGLA
• Synonyms: C11orf11; DAGL(ALPHA); DAGLALPHA; NSDDR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_006133, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCCCGGGATCGTGGTGTTCCGGCGGCGCTGGTCTGTGGGCAGTGATGACCTCGTCCTACCGGCCATCT
  TCCTCTTTCTCCTGCATACCACCTGGTTTGTGATCCTGTCCGTGGTGCTCTTCGGCCTGGTCTATAACCC
  GCACGAGGCCTGCTCCCTGAACCTGGTGGACCACGGCCGCGGCTACCTGGGCATCCTGCTGAGCTGCATG
  ATCGCTGAGATGGCCATCATCTGGCTGAGCATGCGCGGGGGCATCCTCTACACGGAGCCCCGTGACTCCA
  TGCAGTACGTGCTCTACGTGCGCCTGGCCATCCTGGTGATCGAGTTCATCTACGCCATCGTGGGCATCGT
  CTGGCTCACTCAGTACTACACCTCCTGCAACGACCTCACTGCCAAGAATGTCACCCTCGGAATGGTTGTC
  TGCAACTGGGTAGTCATCCTCAGTGTGTGCATCACTGTCCTCTGCGTCTTCGACCCCACGGGCCGCACCT
  TTGTCAAGCTGAGAGCCACCAAGAGGAGGCAGCGTAACCTGCGGACCTACAACCTGCGGCACCGCTTAGA
  GGAGGGTCAAGCCACCAGCTGGTCGCGCCGGCTCAAAGTGTTCCTCTGCTGCACGCGGACGAAGGACTCC
  CAGTCAGATGCCTACTCAGAAATCGCCTACCTCTTTGCGGAGTTCTTCCGGGACCTTGACATTGTGCCAT
  CCGACATCATTGCTGGCCTGGTGCTGCTCCGGCAGCGGCAGCGGGCCAAGCGCAACGCCGTGCTGGACGA
  GGCAAACAATGACATCTTGGCCTTCCTGTCTGGGATGCCGGTGACCAGAAACACCAAGTACCTCGACCTC
  AAGAATTCACAAGAGATGCTCCGCTACAAAGAGGTCTGCTACTACATGCTCTTTGCCCTGGCTGCCTACG
  GGTGGCCCATGTACCTGATGCGGAAGCCCGCCTGCGGCCTCTGCCAACTGGCTCGGTCCTGCTCGTGTTG
  CCTGTGTCCTGCGAGGCCGCGGTTCGCCCCTGGAGTCACCATCGAGGAAGACAACTGCTGTGGCTGTAAT
  GCCATTGCCATCCGGCGCCACTTCCTGGACGAGAACATGACTGCGGTGGACATCGTCTATACCTCCTGCC
  ATGATGCGGTCTATGAAACGCCCTTCTACGTGGCGGTGGACCATGACAAGAAGAAAGTGGTGATCAGTAT
  CCGGGGGACCCTGTCCCCCAAGGATGCCCTGACTGACCTGACGGGTGATGCTGAGCGCCTCCCCGTGGAG
  GGGCACCACGGCACCTGGCTGGGCCACAAGGGTATGGTCCTCTCAGCTGAGTACATCAAGAAGAAACTGG
  AGCAGGAGATGGTCCTGTCCCAGGCCTTTGGGCGAGACCTGGGCCGCGGAACCAAACACTACGGCCTGAT
  TGTGGTGGGCCACTCCCTGGGCGCGGGCACTGCTGCCATCCTCTCCTTCCTTCTGCGCCCACAGTATCCG
  ACCCTCAAGTGCTTTGCCTACTCCCCGCCAGGGGGCCTGCTGAGTGAGGATGCGATGGAGTATTCCAAGG
  AGTTCGTGACTGCTGTGGTTCTGGGCAAAGACCTCGTCCCCAGGATTGGCCTCTCTCAGCTGGAAGGCTT
  CCGCAGACAGCTCCTGGATGTCCTGCAGCGAAGCACCAAGCCCAAATGGCGGATCATCGTGGGGGCCACC
  AAATGCATCCCCAAGTCGGAGCTGCCTGAGGAGGTAGAGGTGACCACCCTGGCCAGCACGCGGCTCTGGA
  CCCACCCCAGCGACCTAACTATAGCCCTCTCAGCCAGCACTCCACTCTACCCGCCCGGCCGCATCATCCA
  CGTGGTCCACAACCACCCTGCAGAGCAGTGCTGCTGCTGTGAGCAGGAGGAGCCCACATACTTTGCCATC
  TGGGGCGACAACAAGGCCTTCAATGAGGTGATCATCTCGCCAGCCATGCTGCATGAGCACCTGCCCTATG
  TGGTCATGGAGGGGCTCAACAAGGTGCTGGAGAACTACAACAAGGGGAAGACCGCTCTGCTCTCTGCAGC
  CAAGGTCATGGTGAGCCCTACCGAGGTGGACCTGACTCCTGAGCTCATCTTCCAGCAGCAGCCACTCCCC
  ACGGGGCCGCCCATGCCCACTGGCCTTGCCCTGGAGCTGCCGACTGCAGACCACCGCAACAGCAGCGTCA
  GGAGCAAGTCCCAGTCTGAGATGAGCCTGGAGGGCTTCTCGGAGGGGCGGCTGCTGTCGCCAGTGGTTGC
  GGCGGCGGCCCGCCAGGACCCGGTGGAGCTGCTGCTGCTGTCTACCCAGGAGCGGCTGGCGGCGGAGCTG
  CAGGCCCGGCGGGCACCACTGGCCACCATGGAGAGCCTCTCGGACACTGAGTCCCTGTACAGCTTCGACT
  CGCGCCGCTCCTCAGGCTTCCGCAGCATCCGGGGCTCCCCCAGCCTCCACGCTGTGCTGGAGCGTGATGA
  AGGCCACCTCTTCTACATTGACCCTGCCATCCCCGAGGAAAACCCATCCCTGAGCTCGCGCACTGAGCTG
  CTGGCGGCCGACAGCCTGTCCAAGCACTCACAGGACACGCAGCCCCTGGAGGCGGCCCTGGGCAGTGGCG
  GCGTCACTCCTGAGCGGCCCCCCAGTGCTGCGGCCAATGACGAGGAGGAAGAGGTTGGCGGTGGGGGTGG
  CGGGCCGGCCTCCCGCGGGGAGCTGGCGCTGCACAATGGGCGCCTGGGGGACTCGCCCAGTCCTCAGGTG
  CTGGAATTCGCCGAGTTCATCGACAGCCTCTTCAACCTGGACAGCAAGAGCAGCTCCTTCCAAGACCTCT
  ACTGCATGGTGGTGCCCGAGAGCCCCACCAGTGACTACGCTGAGGGCCCCAAGTCCCCCAGCCAGCAAGA
  GATCCTGCTCCGTGCCCAGTTCGAGCCCAACCTGGTGCCCAAGCCCCCACGGCTCTTTGCCGGCTCAGCC
  GACCCCTCCTCGGGCATCTCACTCTCGCCCTCCTTCCCGCTCAGCTCCTCGGGTGAGCTCATGGACCTGA
  CGCCCACGGGCCTCAGTAGCCAGGAATGCCTGGCGGCTGACAAGATCCGGACTTCTACCCCCACTGGCCA
  CGGAGCCAGCCCCGCCAAGCAAGATGAGCTGGTCATCTCAGCACGCTAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_006133
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006133.2, NP_006124.1
• RefSeq Size: 5761 bp
• RefSeq ORF: 3129 bp
• Locus ID: 747
• Cytogenetics: 11q12.2
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: 'This gene encodes a diacylglycerol lipase. The encoded enzyme is involved in the biosynthesis of the endocannabinoid 2-arachidonoyl-glycerol.[provided by RefSeq, Nov 2010]'