Proteasome 20S beta 6 (PSMB6) (NM_002798) Human Untagged Clone

CAT#: SC101180

PSMB6 (untagged)-Human proteasome (prosome, macropain) subunit, beta type, 6 (PSMB6)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PSMB6
• Synonyms: DELTA; LMPY; Y
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC101180 sequence for NM_002798 edited (data generated by NextGen Sequencing)
  ATGGCGGCTACCTTACTAGCTGCTCGGGGAGCCGGGCCAGCACCGGCTTGGGGGCCGGAG
  GCGTTCACTCCAGACTGGGAAAGCCGAGAAGTTTCCACTGGGACCACTATCATGGCCGTG
  CAGTTTGACGGGGGCGTGGTTCTGGGGGCGGACTCCAGAACAACCACTGGGTCCTACATC
  GCCAATCGAGTGACTGACAAGCTGACACCTATTCACGACCGCATTTTCTGCTGTCGCTCA
  GGCTCAGCTGCTGATACCCAGGCAGTAGCTGATGCTGTCACCTACCAGCTCGGTTTCCAC
  AGCATTGAACTGAATGAGCCTCCACTGGTCCACACAGCAGCCAGCCTCTTTAAGGAGATG
  TGTTACCGATACCGGGAAGACCTGATGGCGGGAATCATCATCGCAGGCTGGGACCCTCAA
  GAAGGAGGGCAGGTGTACTCAGTGCCTATGGGGGGTATGATGGTAAGGCAGTCCTTTGCC
  ATTGGAGGCTCCGGGAGCTCCTACATCTATGGCTATGTTGATGCTACCTACCGGGAAGGC
  ATGACCAAGGAAGAGTGTCTGCAATTCACTGCCAATGCTCTCGCTTTGGCCATGGAGCGG
  GATGGCTCCAGTGGAGGAGTGATCCGCCTGGCAGCCATTGCAGAGTCAGGGGTAGAGCGG
  CAAGTACTTTTGGGAGACCAGATACCCAAATTCGCCGTTGCCACTTTACCACCCGCCTGA
  
  Clone variation with respect to NM_002798.1
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002798 unedited
  TTCAAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAAATTCGCACGAGGAAAGAGG
  CGGCTACCTTACTAGCTGCTCGGGGAGCCGGGCCAGCACCGGCTTGGGGGCCGGAGGCGT
  TCACTCCAGACTGGGAAAGCCGAGAAGTTTCCACTGGGACCACTATCATGGCCGTGCAGT
  TTGACGGGGGCGTGGTTCTGGGGGCGGACTCCAGAACAACCACTGGGTCCTACATCGCCA
  ATCGAGTGACTGACAAGCTGACACCTATTCACGACCGCATTTTCTGCTGTCGCTCAGGCT
  CAGCTGCTGATACCCAGGCAGTAGCTGATGCTGTCACCTACCAGCTCGGTTTCCACAGCA
  TTGAACTGAATGAGCCTCCACTGGTCCACACAGCAGCCAGCCTCTTTAAGGAGATGTGTT
  ACCGATACCGGGAAGACCTGATGGCGGGAATCATCATCGCAGGCTGGGACCCTCAAGAAG
  GAGGGCAGGTGTACTCAGTGCCTATGGGGGGTATGATGGTAAGGCAGTCCTTTGCCATTG
  GAGGCTCCGGGAGCTCCTACATCTATGGCTATGTTGATGCTACCTACCGGGAAGGCATGN
  ACCAAGAAGAGTGTCTGCAATTCACTGCCAATGCTCTCGCTTTGGCCATGGAGCGGGATG
  GCTCCAGTGGAGGAGTGATCCGCCTGGCAGCCATTGCAGAGTCAGGGGTAGAGCGGCAAG
  TACTTTTGGGAGACCAGATACCCAAATTCGCCGTTGCCACTTTACCACCCGNCTGAATCC
  TGNGATTCTAGTATGCAATAAGAGATGCCCTGTACTGATGCAAAATTAAATAAGNTTGTC
  ACAGAGAAAAAAAAAAAAAAAACTCGACTCTAGATTGGGNCGCGGTCATAGCTGNTTCCT
  GAACAGACCCGGNTGGCATNNCTGTTGACCCTNCCAGTNCTTTNCTGGGCCTGGAANNNT
  GCACTCATGCN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002798
• Insert Size: 800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002798.1, NP_002789.1
• RefSeq Size: 849 bp
• RefSeq ORF: 720 bp
• Locus ID: 5694
• Cytogenetics: 17p13.2
• Domains: proteasome
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Proteasome
• Gene Summary: 'The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. The encoded protein is a member of the proteasome B-type family, also known as the T1B family, and is a 20S core beta subunit in the proteasome. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]'
  Transcript Variant: This variant (1) encodes the longer isoform (1).