Fbx32 (FBXO32) (NM_148177) Human Untagged Clone

CAT#: SC101194

FBXO32 (untagged)-Human F-box protein 32 (FBXO32), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FBXO32
• Synonyms: Fbx32; MAFbx
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC101194 sequence for NM_148177 edited (data generated by NextGen Sequencing)
  ATGAATATTTTGGAAAAAGTGGTACTGAAAGTCCTTGAAGACCAGCAAAACATTAGACTA
  ATAAGGGAACTACTCCAGACCCTCTACACATCCTTATGTACACTGGTCCAAAGAGTCGGC
  AAGTCTGTGCTGGTCGGGAACATTAACATGTGGGTGTATCGGATGGAGACGATTCTCCAC
  TGGCAGCAGCAGCTGAACAACATTCAGATCACCAGGCCTGCCTTCAAAGGCCTCACCTTC
  ACTGACCTGCCTTTGTGCCTACAACTGAACATCATGCAGAGGCTGAGCGACGGGCGGGAC
  CTGGTCAGCCTGGGCCAGGCTGCCCCCGACCTGCACGTGCTCAGCGAAGACCGGCTGCTG
  TGGAAGAAACTCTGCCAGTACCACTTCTCCGAGCGGCAGATCCGCAAACGATTAATTCTG
  TCAGACAAAGGGCAGCTGGATTGGAAGAAGATGTATTTCAAACTTGTCCGATGTTACCCA
  AGGAAAGAGCAGTATGGAGATACCCTTCAGCTCTGCAAACACTGTCACATCCTTTCCTGG
  AAGGGCACTGACCATCCGTGCACTGCCAATAACCCAGAGAGCTGCTCCGTTTCACTTTCA
  CCCCAGGACTTTATCAACTTGTTCAAGTTCTGA
  
  Clone variation with respect to NM_148177.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_148177 unedited
  TTGATACATCCTTAGGCGGCCGGAATCGCCGAGGTGGCATCGCCCAAAAAACTATGAATA
  TTTTGGAAAAGTGGTACTGAAAGTCCTTGAAGACCAGCAAAACATTAGACTAATAAGGGA
  ACTACTCCAGACCCTCTACACATCCTTATGTACACTGGTCCAAAGAGTCGGCAAGTCTGT
  GCTGGTCGGGAACATTAACATGTGGGTGTATCGGATGGAGACGATTCTCCACTGGCAGCA
  GCAGCTGAACAACATTCAGATCACCAGGCCTGCCTTCAAAGGCCTCACCTTCACTGACCT
  GCCTTTGTGCCTACAACTGAACATCATGCAGAGGCTGAGCGACGGGCGGGACCTGGTCAG
  CCTGGGCCAGGCTGCCCCCGACCTGCACGTGCTCAGCGAAGACCGGCTGCTGTGGAAGAA
  ACTCTGCCAGTACCACTTCTCCGAGCGGCAGATCCGCAAACGATTAATTCTGTCAGACAA
  AGGGCAGCTGGATTGGAAGAAGATGTATTTCAAACTTGTCCGATGTTACCCAAGGAAAGA
  GCAGTATGGAGATACCCTTCAGCTCTGCAAACACTGTCACATCCTTTCCTGGAAGGGCAC
  TGACCATCCGTGCACTGCCAATAACCCAGAGAGCTGCTCCGTTTCACTTTCACCCCAGGA
  CTTTATCAACTTGNTCAAGTTCTGAATCCCAGCACATGACAACACTTCAGAAAGGTCCCC
  CTGCTGACTGGAGAGCTGGGAATATGGCATTTGGACACTTCATTTGTAATAGTGTACATT
  TTAAACATTGGCTCGAAACTTCANAGATAAGTCATGGAGAAGACATTGNAGGGGAGAAAT
  GCATTGCTGACTGGGAAATTAAGAAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_148177 unedited
  TGGTGTGNATTGGCCCCNGTTAGGGATTTNCTAGTTATTTTTTTTTTTTTTTCTAAACAA
  ATTTTCACATTGTAAACAAAGCGTCCTATTTTGTATTTGAGTTTTGCCTTTTTCTTCCTA
  GGGAACTATAAGAGAGTTTCTGTGACCATTAAGAGGACTCCATTTTCACCGCTGAAATAG
  AATTATTGCCCTTATAGTCTTGCTGGCAAATTGTTAGAAAAAAAGTTTATTTACAGTATT
  TTGCTTTTCCATACCAATTCTAGTGAGAAGTTCACATTCTTAAATTCCCAGTCAGCAACT
  GCATTTCTCCCCTCCAATGTCCTCTCCATGACTTATCTCTGAAGTTTCGAGCCAATGTTT
  AAAATGTACACTATTTACAAATGAAGTGTCCAAATGCCATATTCCCAGCTCTCCAGTCAG
  CAGGGGGACCCTTCTGAAGTGTTGTCATGTGCTGGGATTCAGAACTTGAACAAGTTGATA
  AAGTCCTGGGGTGAAAGTGAAACGGAGCAGCTCTCTGGGTTATTGGCAGTGCACGGATGG
  TCAGTGCCCTTCCAGGAAAGGATGTGACAGTGTTTGCAGAGCTGAAGGGTATCTCCATAC
  TGCTCTTTCCTTGGGTAACATCGGACAAGTTTGAAATACATCTTCTTCCAATCCAGCTGC
  CCTTTGTCTGACAGAATTAATCGTTTGCGGATCTGCCGCTCGGAGAAGTGGTACTGGCAG
  AGTTTCTTCCACAGCAGCCGGTCTTCCCTGAGCACGTGCAGGTCGGGGGCAGCCTGGCCC
  AGGCTGACCAGGTCCCGCCCGTCGCTCAGCCTCTGCATGATGTTCAGGTGGAGGGCACAA
  AGCCGGTCATTGGAAGGGAGGCCCTTTAAGGCAGGCCTGGGGATCTGAATGTTGTTCAAC
  TGCTGCTGCCAGTGGGAAATTCTCCTCCTTCCGATAACCCCCAGTTGAAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_148177
• ORF Size: 633 bp
• Insert Size: 1150
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_148177.1, NP_680482.1
• RefSeq Size: 1212
• RefSeq ORF: 633
• Locus ID: 114907
• Domains: F-box
• Gene Summary: This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class and contains an F-box domain. This protein is highly expressed during muscle atrophy, whereas mice deficient in this gene were found to be resistant to atrophy. This protein is thus a potential drug target for the treatment of muscle atrophy. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]
  Transcript Variant: This variant (2) has a shorter and alternate 5' terminal sequence lacking an in-frame translation start codon, as compared to variant 1. It uses a downstream in-frame translation start codon and encodes a shorter isoform (2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.