MANEA (AY048775) Human Untagged Clone
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Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Symbol | MANEA |
Synonyms | ENDO|hEndo |
Vector | pCMV6-XL4 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>NCBI ORF sequence for AY048775, the custom clone sequence may differ by one or more nucleotides
ATGGCAAAGTTTCGGAGAAGGACTTGCATCATTTTGGCACTTTTTATTCTATTTATTTTCTCTCTGATGA TGGGTTTAAAAATGCTGAGACCAAATACAGCTACTTTTGGAGCTCCTTTTGGACTTGACCTTCTTCCAGA ACTTCATCAACGAACTATTCATTTGGGGAAAAATTTTGATTTCCAAAAGAGTGACAGAATCAACAGTGAA ACAAATACCAAGAATTTAAAAAGTGTTGAAATCACTATGAAACCTTCCAAAGCCTCTGAACTTAACTTGG ATGAACTACCACCTCTGAACAATTATCTACATGTATTTTATTACAGTTGGTATGGAAATCCACAATTTGA TGGTAAATATATACATTGGAATCATCCAGTGTTAGAGCATTGGGACCCTAGAATAGCCAAGAATTATCCA CAAGGGAGACACAACCCTCCAGATGACATTGGCTCCAGCTTTTATCCTGAATTGGGAAGTTACAGTTCTC GGGATCCTTCTGTCATAGAAACTCACATGAGACAAATGCGCTCAGCTTCAATTGGTAATTATTGTATATA TATATATATATGTGTGTTTGTGTCTGTATATATGCATATAAATGATTTTTTGTGTAACTTTACTAGTTAA >OriGene 5' read for AY048775 unedited
GTCAGCAATTGTATACGACTCACTATAGGCGGCCGCGATTCGGCACGAGCCTGAAACGCC CGGAACGCTGTGCGCGCGCCTTCGGGGAGGAGGGGCTGTCTGGGCTCGGGGCGCGGCGGC AGTCAGCTCTATGTTCGCGGTCTTAACCTCTCCTCTGGCCGAGTCCTTGCAAGAAGTGAA TTACCCGACCCTGCAAAACACTTACTATTTTGGAGTTTAAAGTATGTCATCATGGCAAAG TTTCGGAGAAGGACTTGCATCATTTTGGCACTTTTTATTCTATTTATTTTCTCTCTGATG ATGGGTTTAAAAATGCTGAGACCAAATACAGCTACTTTTGGAGCTCCTTTTGGACTTGAC CTTCTTCCAGAACTTCATCAACGAACTATTCATTTGGGGAAAAATTTTGATTTCCAAAAG AGTGACAGAATCAACAGTGAAACAAATACCAAGAATTTAAAAAGTGTTGAAATCACTATG AAACCTTCCAAAGCCTCTGAACTTAACTTGGATGAACTACCACCTCTGAACAATTATCTA CATGTATTTTATTACAGTTGGTATGGAAATCCACAATTTGATGGTAAATATATACATTGG AATCATCCAGTGTTAGAGCATTGGGACCCTAGAATAGCCAAGAATTATCCACAAGGGAGA CACAACCCTCCAGATGACATTGGCTCCAGCTTTTATCCTGAATTGNGAAGTTACAGTTCT CGGGATCCTTCTGTCATAGAAACTCACATGAGACAAATGCGCTCAGCTTCAATTGGTGTA CTAGCCCTCTCTTGGTACCCACCTGATGTAAATGATGAAAATGGAGAACCTACTGATNAC TGGNTACCCACTANTTTGGATAAAGCTCATAAATATAAACCTANAGGNNNTACTTTCACA TAGAACCCATATAGCATCGAGATGATCAAAACATGTACANAANATGTCAGTATATTATAG ACAAA |
Restriction Sites | NotI-NotI |
ACCN | AY048775 |
ORF Size | 630 bp |
Insert Size | 4530 |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Reference Data | |
RefSeq | AY048775.1, AAL07307.1 |
RefSeq Size | 1096 |
RefSeq ORF | 630 |
Locus ID | 79694 |
Protein Families | Transmembrane |
Gene Summary | N-glycosylation of proteins is initiated in the endoplasmic reticulum (ER) by the transfer of the preassembled oligosaccharide glucose-3-mannose-9-N-acetylglucosamine-2 from dolichyl pyrophosphate to acceptor sites on the target protein by an oligosaccharyltransferase complex. This core oligosaccharide is sequentially processed by several ER glycosidases and by an endomannosidase (E.C. 3.2.1.130), such as MANEA, in the Golgi. MANEA catalyzes the release of mono-, di-, and triglucosylmannose oligosaccharides by cleaving the alpha-1,2-mannosidic bond that links them to high-mannose glycans (Hamilton et al., 2005 [PubMed 15677381]). [supplied by OMIM, Sep 2008] |
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