Cytokeratin 4 (KRT4) (NM_002272) Human Untagged Clone

CAT#: SC102891

KRT4 (untagged)-Human keratin 4 (KRT4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KRT4
• Synonyms: CK-4; CK4; CYK4; K4; WSN1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC102891 sequence for NM_002272 edited (data generated by NextGen Sequencing)
  ATGATTGCCAGACAGCAGTGTGTCCGAGGCGGGCCCCGGGGCTTCAGCTGTGGCTCGGCC
  ATTGTAGGNGGNNNNNNNAGAGGTGCCTTCAGCTCAGTCTCCATGTCTGGAGGTGCTGGC
  CGATGCTCTTCTGGGGGATTTGGCAGCAGAAGCCTCTACAACCTCAGGGGGAACAAAAGC
  ATCTCCATGAGTGTGGCTGGGTCACGACAAGGTGCCTGCTTTGGGGGTGCTGGAGGCTTT
  GGCACTGGTGGCTTTGGTGGTGGATTTGGGGGCTCCTTCAGTGGTAAGGGTGGCCCTGGC
  TTCCCCGTCTGCCCCGCTGGGGGAATTCAGGAGGTCACCATCAACCAGAGCTTGCTCACC
  CCCCTCCACGTGGAGATTGACCCTGAGATCCAGAAAGTCCGGACGGAAGAGCGCGAACAG
  ATCAAGCTCCTCAACAACAAGTTTGCCTCCTTCATCGACAAGGTGCAGTTCTTAGAGCAA
  CAGAATAAGGTCCTGGAGACCAAATGGAACCTGCTCCAGCAGCAGACGACCACCACCTCC
  AGCAAAAACCTTGAGCCCCTCTTTGAGACCTACCTCAGTGTCCTGAGGAAGCAGCTAGAT
  ACCTTGGGCAATGACAAAGGGCGCCTGCAGTCTGAGCTGAAGACCATGCAGGACAGCGTG
  GAGGACTTCAAGACTAAGTATGAAGAGGAGATCAACAAACGCACAGCAGCCGAGAATGAC
  TTTGTGGTCCTAAAGAAGGACGTGGATGCTGCCTACCTGAACAAGGTGGAGTTGGAGGCC
  AAGGTGGACAGTCTTAATGACGAGATCAACTTCCTGAAGGTCCTCTATGATGCGGAGCTG
  TCCCAGATGCAGACCCATGTCAGCGACACGTCCGTGGTCCTTTCCATGGACAACAACCGC
  AACCTGGACCTGGACAGCATTATTGCCGAGGTCCGTGCCCAGTACGAGGAGATTGCCCAG
  AGGAGCAAGGCTGAGGCTGAAGCCCTGTACCAGACCAAGGTCCAGCAGCTCCAGATCTCG
  GTTGACCAACATGGTGACAACCTGAAGAACACCAAGAGTGAAATTGCAGAGCTCAACAGG
  ATGATCCAGAGGCTGCGGGCAGAGATCGAGAACATCAAGAAGCAGTGCCAGACTCTTCAG
  GTATCCGTGGCTGATGCAGAGCAGCGAGGTGAGAATGCCCTTAAAGATGCCCACAGCAAG
  CGCGTAGAGCTGGAGGCTGCCCTGCAGCAGGCCAAGGAGGAGCTGGCACGAATGCTGCGT
  GAGTACCAGGAGCTCATGAGTGTGAAGCTGGCCTTGGACATCGAGATCGCCACCTACCGC
  AAACTGCTGGAGGGCGAGGAGTACAGAATGTCTGGAGAATGCCAGAGTGCCGTGAGCATC
  TCTGTGGTCAGCGGTAGCACCAGCACTGGAGGCATCAGCGGAGGATTAGGAAGTGGCTCC
  GGGTTTGGCCTGAGTAGTGGCTTTGGCTCCGGCTCTGGAAGTGGCTTTGGGTTTGGTGGC
  AGTGTCTCTGGCAGTTCCAGCAGCAAGATCATCTCTACCACCACCCTGAACAAGAGACGA
  TAG
  
  Clone variation with respect to NM_002272.3
  69 c=>n;72 t=>n;73 g=>n;74 g=>n;75 c=>n;76 a=>n;77 a=>n;78 g=>n
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002272 unedited
  GGTTGCGNATTTGTATACGATCCTATAGGCGGCCCGCGATTCGGCACCAGCACTTCTCTG
  ATAGCTCCCAGCTCGCTCTCTGCAGCCATGATTGCCAGACAGCAGTGTGTCCGAGGCGGG
  CCCCGGGGCTTCAGCTGTGGCTCGGCCATTGTAGGCGGTGGCAAGAGAGGTGCCTTCAGC
  TCAGTCTCCATGTCTGGAGGTGCTGGCCGATGCTCTTCTGGGGGATTTGGCAGCAGAAGC
  CTCTACAACCTCAGGGGGAACAAAAGCATCTCCATGAGTGTGGCTGGGTCACGACAAGGT
  GCCTGCTTTGGGGGTGCTGGAGGCTTTGGCACTGGTGGCTTTGGTGCCGGCGGCTTCGGA
  GCTGGTTTCGGCACTGGTGGCTTTGGTGGTGGATTTGGGGGCTCCTTCAGTGGTAAGGGT
  GGCCCTGGCTTCCCCGTCTGCCCCGCTGGGGGAATTCAGGAGGTCACCATCAACCAGAGC
  TTGCTCACCCCCCTCCACGTGGAGATTGACCCTGAGATCCAGAAAGTCCGGACGGAAGAG
  CGCGAACAGATCAACCTCCTCAACAACAAGTTTGCCTCCTTCATCGACAAGGTGCAGTTC
  TTAGAGCAACAGAATAAGGTCCTGGAGACCAAATGGAACCTGCTCCACCAGCAGACGACC
  ACCACCTCCAGCANAAACCTTGAGCCCCTCTTTGAGACCTACCTCAGTGTCCTGAGGAAG
  CAGCTAGATACCTTGGGCATTGACAAAGGGCGCCTGCAGTCTGAGCTGAAGACCATGCAN
  GCCAGCGTGGAGGACTTCAAGACTAAGTATGGAGAGGAGATCAACAAACGCACAGCAGCC
  CGAGAAGACTTTTGTGGTCCTAAAGAAAGACGGGNATGCTGCCTCACTGGACANGTTGNA
  GTTGTAGGCCAGGTGGACAGTCTTATGAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002272 unedited
  AACTAGCTATGCACGCGCGGCCCGTCATACTACGACCCGAGCTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTGAAGGACCAAAGCATTTTATTGAAGATTCACCTGCAGATGGA
  TAACAGGGACAGAGAGGAGTACACATGGGACACTAAGTGGTTCTCATCCTAAGCTTGCAG
  GGCCAAGTGCTGCCGGGTGTTGGACAAGTACTTTGGTTCTGATGTACATGGATAATACAG
  GATCTAGTGGGAGATGGCATTGGACTGGGAAGGGACATATGTGACCCCAATAATTCTGGA
  GAGGACACTCCTGGGCATTTTCTTCTCTGCCCATGGGAGGTGAGAGGTCAGCTGACATCC
  TTCCCATTCCAGGGGGGTCACCAGGCCCAAATCAAGAAGTAGCTACCCAACTCCAAGAGG
  CAGAGTCCCTGTCCCAGCACAGAAGATTATTCTGGGGCAAAGAGAGCCCATGGGATAGTG
  GAGGGGATACTTGTCAGATGAGCCCCCCAACAGAGGATGGAGGTGAAGTGCAGGACCCAC
  CCAGACACCAGTGCTGGCCCCAGCTGGACACCGCGAGCCTGCGGGCCCCCCTCTCCTCTA
  TCGTCTCTTGTCCCGGGTGGTGGTCGAGATGACTCTCCTTGTGGAACTTGCCTACTACCT
  GTCCCCAACCCCCCCCGCCACTTCCTTATCTCTTCCCCTACCCACTTTTACGCCCCCCCC
  TTGTATCCCCCCCAAACACCCTCCCTTCACGCCACCCCCCCATTTTGTTTCTCCTCCCTC
  TACCAACTCTCTCTCCCCCGCCCTTCTCTTATATCCCACACAACCTATTCACCCTCCCCT
  TCTTCCTTTCCCTTCTCTCCTCCATTCTCAACCCCCGCCCGTTACACCCCCCCGCCCCCC
  TCTCTTCACTCATAAAATCATACTTCATTCTACTTCTTCTCCCCCCCCCCATCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002272
• Insert Size: 2170 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002272.1, NP_002263.1
• RefSeq Size: 2617 bp
• RefSeq ORF: 1605 bp
• Locus ID: 3851
• Cytogenetics: 12q13.13
• Domains: filament
• Gene Summary: 'The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in differentiated layers of the mucosal and esophageal epithelia with family member KRT13. Mutations in these genes have been associated with White Sponge Nevus, characterized by oral, esophageal, and anal leukoplakia. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008]'