Cytokeratin 4 (KRT4) (NM_002272) Human Untagged Clone

CAT#: SC318023

KRT4 (untagged)-Human keratin 4 (KRT4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KRT4
• Synonyms: CK-4; CK4; CYK4; K4; WSN1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002272, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGATTGCCAGACAGCAGTGTGTCCGAGGCGGGCCCCGGGGCTTCAGCTGTGGCTCGGCCATTGTAGGCG
  GTGGCAAGAGAGGTGCCTTCAGCTCAGTCTCCATGTCTGGAGGTGCTGGCCGATGCTCTTCTGGGGGATT
  TGGCAGCAGAAGCCTCTACAACCTCAGGGGGAACAAAAGCATCTCCATGAGTGTGGCTGGGTCACGACAA
  GGTGCCTGCTTTGGGGGTGCTGGAGGCTTTGGCACTGGTGGCTTTGGTGGTGGATTTGGGGGCTCCTTCA
  GTGGTAAGGGTGGCCCTGGCTTCCCCGTCTGCCCCGCTGGGGGAATTCAGGAGGTCACCATCAACCAGAG
  CTTGCTCACCCCCCTCCACGTGGAGATTGACCCTGAGATCCAGAAAGTCCGGACGGAAGAGCGCGAACAG
  ATCAAGCTCCTCAACAACAAGTTTGCCTCCTTCATCGACAAGGTGCAGTTCTTAGAGCAACAGAATAAGG
  TCCTGGAGACCAAATGGAACCTGCTCCAGCAGCAGACGACCACCACCTCCAGCAAAAACCTTGAGCCCCT
  CTTTGAGACCTACCTCAGTGTCCTGAGGAAGCAGCTAGATACCTTGGGCAATGACAAAGGGCGCCTGCAG
  TCTGAGCTGAAGACCATGCAGGACAGCGTGGAGGACTTCAAGACTAAGTATGAAGAGGAGATCAACAAAC
  GCACAGCAGCCGAGAATGACTTTGTGGTCCTAAAGAAGGACGTGGATGCTGCCTACCTGAACAAGGTGGA
  GTTGGAGGCCAAGGTGGACAGTCTTAATGACGAGATCAACTTCCTGAAGGTCCTCTATGATGCGGAGCTG
  TCCCAGATGCAGACCCATGTCAGCGACACGTCCGTGGTCCTTTCCATGGACAACAACCGCAACCTGGACC
  TGGACAGCATTATTGCCGAGGTCCGTGCCCAGTACGAGGAGATTGCCCAGAGGAGCAAGGCTGAGGCTGA
  AGCCCTGTACCAGACCAAGGTCCAGCAGCTCCAGATCTCGGTTGACCAACATGGTGACAACCTGAAGAAC
  ACCAAGAGTGAAATTGCAGAGCTCAACAGGATGATCCAGAGGCTGCGGGCAGAGATCGAGAACATCAAGA
  AGCAGTGCCAGACTCTTCAGGTATCCGTGGCTGATGCAGAGCAGCGAGGTGAGAATGCCCTTAAAGATGC
  CCACAGCAAGCGCGTAGAGCTGGAGGCTGCCCTGCAGCAGGCCAAGGAGGAGCTGGCACGAATGCTGCGT
  GAGTACCAGGAGCTCATGAGTGTGAAGCTGGCCTTGGACATCGAGATCGCCACCTACCGCAAACTGCTGG
  AGGGCGAGGAGTACAGAATGTCTGGAGAATGCCAGAGTGCCGTGAGCATCTCTGTGGTCAGCGGTAGCAC
  CAGCACTGGAGGCATCAGCGGAGGATTAGGAAGTGGCTCCGGGTTTGGCCTGAGTAGTGGCTTTGGCTCC
  GGCTCTGGAAGTGGCTTTGGGTTTGGTGGCAGTGTCTCTGGCAGTTCCAGCAGCAAGATCATCTCTACCA
  CCACCCTGAACAAGAGACGATAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_002272
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002272.3, NP_002263.3
• RefSeq Size: 2147 bp
• RefSeq ORF: 1563 bp
• Locus ID: 3851
• Cytogenetics: 12q13.13
• Domains: filament
• Gene Summary: 'The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in differentiated layers of the mucosal and esophageal epithelia with family member KRT13. Mutations in these genes have been associated with White Sponge Nevus, characterized by oral, esophageal, and anal leukoplakia. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008]'