RANTES (CCL5) (AK094877) Human Untagged Clone

CAT#: SC104452

(untagged)-Human cDNA FLJ37558 fis, clone BRCOC1000087

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CCL5
• Synonyms: 4930578C19Rik|DIA1R|EPQL1862|PRO3743|bA435K1.1
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for AK094877, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTTCGGCGTCTTTAGCAACGGGCATCTGTTCATCCGGGATGCCAGTGCAGTGGGCGTCATCGACAAGC
  AGGAAGGCAGCCAAGAAGCCAACAGGGCAGGAGAGAATAAAGACATTTTTAGCTGCCTGGTTTCCGGCTG
  CCAGGCCCAGCTGCCCTCCTGCGAAAGCATCTCTGAGAAGCAGAGCCTGGTGCTGGTGTGTCAGAAGTTG
  CTGCCTCGACTTCTCCAGGGGAGGTTCCCCTCCCCAGTGCAAGACGACATAGACTCCATCCTTGTTCAGT
  GTGGGGACAGCATCCGCCCAGACCCAGAAGTCCTTGGGGCCGCCAGCCAGCTGAAAGACATCTTGAGGCC
  CCTGAGAACGTGTGACTCCAGATTTGCCTATCGTTACCCAGATTGCAAATATAACGATAAGTTCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for AK094877 unedited
  NNTTCATATCCCCCGCCCGTTGACGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTAT
  ATAAGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCG
  GCCGCGAATTCGGCACGAGGAACGATTTGAACTATTTCTTCTACTTCACCCACATTGATG
  CAGGCATGTTCGGCGTCTTTAACAACGGGCATCTGTTCATCCGGGATGCCAGTGCAGTGG
  GCGTCATCGACAAGCAGGAAGGCAGCCAAGAAGCCAACAGGGCAGGAGAGAATAAAGACA
  TTTTTAGCTGCCTGGTTTCCGGCTGCCAGGCCCAGCTGCCCTCCTGCGAAAGCATCTCTG
  AGAAGCAGAGCCTGGTGCTGGTGTGTCAGAAGTTGCTGCCTCGACTTCTCCAGGGGAGGT
  TCCCCTCCCCAGTGCAAGACGACATAGACTCCATCCTTGTTCAGTGTGGGGACAGCATCC
  GCCCAGACCCAGAAGTCCTTGGGGCCGCCAGCCAGCTGAAAGACATCTTGAGGCCCCTGA
  GAACGTGTGACTCCAGATTTGCCTATCGTTACCCAGAGTGCAAATATAACGATAAGTTCT
  GAAGGGCTGGTGTCTAGCTGGCCTTGGGGACAACACGCTTGGCTCTCCATTCTCCCAGTC
  GGGTTGCTGCAAGGTAGAAAGAAGCAGCTCTTTTTTAAAGATGAGAAGAAAGGCACACTC
  ATTTCTTAGGGACTGAGACGGGGNACGTTGTGAGATACCCGGTTGGCTGTCTCTGCTGGC
  TTTGGGACGTCCTGGCAAGCCCCGCGGTGGGCATCAAGACCCTGGGGTTGGTGGACGCTC
  ACTCATGGTCTTGAGAGCTCAGTTTCCTCTATTCTNCACAACTCTCCTTTGGGGGGTGGA
  AAGAAGACACAGATGTAGCCCC
  
• Restriction Sites: NotI-NotI
• ACCN: AK094877
• Insert Size: 4200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: AK094877.1, BAC04447.1
• RefSeq Size: 2354 bp
• RefSeq ORF: 417 bp
• Locus ID: 6352
• Cytogenetics: 17q12
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: Chemokine signaling pathway, Cytokine-cytokine receptor interaction, Cytosolic DNA-sensing pathway, Epithelial cell signaling in Helicobacter pylori infection, NOD-like receptor signaling pathway, Prion diseases, Toll-like receptor signaling pathway
• Gene Summary: 'This gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. This chemokine, a member of the CC subfamily, functions as a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells. It functions as one of the natural ligands for the chemokine receptor chemokine (C-C motif) receptor 5 (CCR5), and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Alternative splicing results in multiple transcript variants that encode different isoforms. [provided by RefSeq, Jul 2013]'