SFRS3 (SRSF3) (AF107405) Human Untagged Clone

CAT#: SC105794

(untagged)-Human pre-mRNA splicing factor (SFRS3) mRNA, complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SRSF3
• Synonyms: SFRS3|SRp20
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for AF107405, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCATCGTGATTCCTGTCCATTGGACTGTAAGGTTTATGTAGGCAATCTTGGAAACAATGGCAACAAGA
  CGGAATTGGAACGGGCTTTTGGCTACTATGGACCACTCCGAAGTGTGTGGGTTGCTAGAAACCCACCCGG
  CTTTGCTTTTGTTGAATTTGAAGATCCCCGAGATGCAGCTGATGCAGTCCGAGAGCTAGATGGAAGAACA
  CTATGTGGCTGCCGTGTAAGAGTGGAACTGTCGAATGGTGAAAAAAGAAGTAGAAATCGTGGCCCACCTC
  CCTCTTGGGGTCGTCGCCCTCGAGATGATTATCGTAGGAGGAGTCCTCCACCTCGTCGCAGATCTCCAAG
  AAGGAGAAGCTTCTCTCGCAGCCGGAGCAGGTCCCTTTCTAGAGATAGGAGAAGAGAGAGATCGCTGTCT
  CGGGAGAGAAATCACAAGCCGTCCCGATCCTTCTCTAGGTCTCGTAGTCGATCTAGGTCAAATGAAAGGA
  AATAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for AF107405 unedited
  TTCAAATTTTGTAATACGACTCACTATAGGGCGGCNCGCGAATTCGCACGAGGGGTGAGT
  GAGAGAGTTGGTTGGTGTTGGGCCGGAGGAAAGCGGGAAGACTCATCGGAGCGTGTGGAT
  TTGAGCCGCCGCATTTTTTAACCCTAGATCTCGAAATGCATCGTGATTCCTGTCCATTGG
  ACTGTAAGGTTTATGTAGGCAATCTTGGAAACAATGGCAACAAGACGGAATTGGAACGGG
  CTTTTGGCTACTATGGACCACTCCGAAGTGTGTGGGTTGCTAGAAACCCACCCGGCTTTG
  CTTTTGTTGAATTTGAAGATCCCCGAGATGCAGCTGATGCAGTCCGAGAGCTAGATGGAA
  GAACACTATGTGGCTGCCGTGTAAGAGTGGAACTGTCGAATGGTGAAAAAAGAAGTAGAA
  ATCGTGGCCCACCTCCCTCTTGGGGTCGTCGCCCTCGAGATGATTATCGTAGGAGGAGTC
  CTCCACCTCGTCGCAGATCTCCAAGAAGGAGAAGCTTCTCTCGCAGCCGGAGCAGGTCCC
  TTTCTAGAGATAGGAGAAGAGAGAGATCGCTGTCTCGGGAGAGAAATCACAAGCCGTCCC
  GATCCTTCTCTAGGTCTCGTAGTCGATCTAGGTCAAATGAAAGGAAATAGAAGACAGTTT
  GCAAGAGAAGTGGTGTACAGGAAATTACTTCATTTGACAGGAGTATGTACAGAAAATTCA
  AGTTTTGTTTGAGACTTCATAAGCTTGGTGCATTTTTAAGATGTTTTAGCTGTTCAAATC
  TGTTTGTCTCTTGAACAGTGACACAAAGTGTATTCTCTATGGTNTGAAATGGACATACGA
  GGCATGTATACCAAGAATGNTACTTTACATGTTCCCTTAGCAAAATGATTTGCTTGAACT
  TTAGTATGCCAGACTGATATAACTCTAACTGCCACGNAGGGTGNTTTTTAAATAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for AF107405 unedited
  GCACGCAATCTATAGTCGAGTTTTTTTTTTTTTTTTTTAACAATTTGTAACCAAAACTTT
  AATCCCAAGGATTCTCACAAAACATATTACAAATGACAGCATGAAAAAAAAATCTTGCAC
  AGTAACTCAAAGTTCGGCTCTACAATGTACCCTTAAACTGGCAGGACATTTTTGAAATCA
  CAAATTTGCACATAAAGAATGTCACGAACAGCCATGTATCCATATACAGCAATCAAATAA
  GGAACTTATGACCTAAAGCAAAGGTAAACTTTCTTGAAACTTAACATTCTATACCAACTA
  GGCAACCTCTGCCCAGGATGAGAGTTGGATTTTTCAAAAACCTCTAATTTAATAGTGCAG
  CATTTCGTTTTCCCTGATGGCCTGTGTTTCACAGCAGTTTTTAAAGACTGCTTGTTCAAC
  TATAGCTGCAGCCTATATCCCAGCTATGGAAAAAAAAGTAAATCTTAGTTCAATTTTTGC
  CAGTTGTTTCTGTATTTAAATTTAAAAAAAAACACACTTCCGCTGGGCAGGTTTAGAGGT
  TTATTATCAGTCTGTGCATAACTAAAAGTTCAAAGCAAATTCAATTTTGCTTAAGGGAAC
  ATTGTAAAGTAACAATTCTTGGTATTACATGCCTCGTATGATCCATTTCAAACCATAGAG
  AATTACACCTTTGTGTCACTGTTTCATGAGACAAACAGATTTGAACAGCTAAACATCTTA
  AAAATGCACCAAGCTTATGAAGTCTCAAACAAAACTTGAATTCTCTGTACATACTCCCTG
  TCAAATGAAGTTATTTTCCTGTACACCACCTCCTCTTGCAAACCTGTCTCCTATTCCCCT
  CCATCTGACCTAGATCGACTACCAGACCTANAGAAAGNATCCGGACGGCTTGTGATTTCT
  CTTCCGAAAAGCGATCCCTCTCTTTCCTATCCTATAAAGGACCGCTCCGCTGGAAAGAAC
  TTCTCTCTGGGGATCGGACAGTGAAGACCCTCTACAATN
  
• Restriction Sites: NotI-NotI
• ACCN: AF107405
• Insert Size: 1430 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: AF107405.1, AAD44523.1
• RefSeq Size: 2060 bp
• RefSeq ORF: 495 bp
• Locus ID: 6428
• Cytogenetics: 6p21.31-p21.2
• Domains: RRM
• Protein Families: Stem cell - Pluripotency
• Protein Pathways: Spliceosome
• Gene Summary: 'The protein encoded by this gene is a member of the serine/arginine (SR)-rich family of pre-mRNA splicing factors, which constitute part of the spliceosome. Each of these factors contains an RNA recognition motif (RRM) for binding RNA and an RS domain for binding other proteins. The RS domain is rich in serine and arginine residues and facilitates interaction between different SR splicing factors. In addition to being critical for mRNA splicing, the SR proteins have also been shown to be involved in mRNA export from the nucleus and in translation. Two transcript variants, one protein-coding and the other non-coding, have been found for this gene. [provided by RefSeq, Sep 2010]'