HuC (ELAVL3) (NM_032281) Human Untagged Clone

CAT#: SC105836

ELAVL3 (untagged)-Human ELAV (embryonic lethal, abnormal vision, Drosophila)-like 3 (Hu antigen C) (ELAVL3), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ELAVL3
• Synonyms: HUC; HUCL; PLE21
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_032281, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGTCACTCAGATACTGGGGGCCATGGAGTCTCAGGTGGGGGGGGGCCCGGCCGGCCCGGCCCTGCCCA
  ACGGGCCACTCCTTGGTACAAATGGAGCCACTGACGACAGCAAGACCAACCTCATCGTCAACTACCTGCC
  CCAGAACATGACCCAGGATGAGTTCAAGAGTCTCTTCGGCAGCATTGGCGACATCGAGTCCTGCAAGTTG
  GTTCGGGACAAGATCACAGGGCAGAGCCTTGGCTACGGGTTTGTGAACTATTCTGACCCCAATGATGCAG
  ACAAAGCCATCAACACCCTCAACGGCCTCAAATTACAGACGAAGACCATCAAGGTGTCCTATGCCAGACC
  CAGTTCAGCATCCATCCGGGATGCTAACCTGTACGTCAGCGGGCTCCCCAAGACCATGAGCCAGAAAGAG
  ATGGAGCAGCTCTTCTCCCAGTACGGCCGCATCATCACGTCCCGCATCCTGGTGGACCAGGTCACAGGTG
  TCTCTCGGGGTGTGGGATTCATCCGCTTTGACAAGAGGATTGAGGCCGAAGAGGCTATCAAAGGACTGAA
  TGGGCAGAAGCCGCTGGGCGCAGCTGAGCCCATCACAGTCAAGTTCGCGAACAACCCAAGTCAGAAGACG
  GGGCAGGCGCTGCTCACCCACCTCTACCAGTCATCCGCCCGGCGCTACGCAGGCCCCCTACACCATCAGA
  CCCAGCGTTTCCGGCTGGACAATTTGCTCAACATGGCCTACGGCGTCAAGAGGTTCTCGCCGATCGCCAT
  CGATGGTATGAGCGGCCTGGCGGGCGTGGGCCTGTCGGGGGGCGCGGCGGGCGCCGGCTGGTGCATCTTC
  GTGTACAACCTGTCACCGGAGGCAGACGAGAGCGTGCTGTGGCAGCTGTTCGGGCCTTTTGGGGCAGTCA
  CCAACGTCAAGGTCATCCGTGATTTCACCACCAACAAGTGCAAGGGTTTCGGCTTCGTGACCATGACCAA
  CTATGACGAGGCGGCCATGGCCATCGCCAGCCTGAACGGCTATCGCCTGGGCGAGCGCGTGCTGCAGGTC
  TCCTTCAAGACCAGCAAACAGCACAAGGCGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_032281 unedited
  GCACGAGGGGGAAAAAGACCTCGTGGAGAATTTTTACTGGGGATTCTTGAACTTGAAAAA
  AAAAAACACAAAAAAAGACAAAAAAAAAAAAGAAATATTTTGGCAGGTTATGTTTACCAA
  CTGGGGCGGGGGTGGGGGGGGCCCAGGGAGCAGGGCTTAGGGGCTAGCAGCCCACGGGGC
  CACACAGAGAAACAACCACGCAGACAGTCACACCACGGGGACACACGGACAGACGCAGAC
  GGACACAGCGACATGCCCCAGACACGTTAAGGGACTGGTTGGCCAAACTCAGACACGTGG
  ACAGGGATAGACAGAAAGAGACAGAAGCTGGGGCTACGTCCATGTGGACACAGACCACAG
  ATGTGGGCACACGAATCCATCCACCTGTCCACGTGCACACGTGAATGTAGCGATAGATAT
  TTGGACATCAAATTTGGACACCAGGTCACGGAGAAACACAAACACCACCCAGGAACACGC
  AGACATATGCCCATAGCATCCCACAGGCCCAGGCAGGAGGTCCCACCCCAGACCCTGCCC
  CAGACGCTCCCTCACCCTCTCGGCCCCCTCGCCCTGGCCCCCAGGTTCTCTGCAGAGATC
  TCTCCTGGACCCCCAGCGGTCTCCTTGGCGCCCACGACACNAGGGCGTGCACACGCAGCG
  CACATGCATTGAGACACACGATACCTCGTTCCACCTTGGCGTCACGGTGGGGCTGGAGGC
  AGCCCCCCCCCCCACAACACTTGAGGCCAAAGGACACCCCTGTCCCCGGN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_032281 unedited
  GCGGCCGCAATCTANAGTCGAGTTNTTNTTTTTTTTTTTTTTTTTTTTTTTTTAAAAACT
  CTTCTGGGGGTTTTCTTTTTTTGGTGCTTTTTTTTCCTCTTCTGTTGGGGTTTTTTACGC
  GCTTTTTTGATTTTTTTCTTTTTTGTTCCTTGCAGCAAAAGCTCCACAGACGTTTAATAA
  CAACACCGGGAGGGGTGGGGGAAAAAGAGCTGGACAGTCAAATGGGGGCGCCATAGCCAG
  ACACCAAACGTGGGGACACCGGGGGAAGGCAACTTAACTATAGCTAAAGTCGGGGGTTTT
  GCAATTCTCAGTTCCGATGGGGCCGCTGAGTGGGGTGGGGATCGGTGCAAGGGCTCCCAG
  GGGCTCCGGCCTGTGGCTTCTGTTGGAATTTTTTTTTTTTAAAGAAAGAAAACTAGGGCG
  ATGCAATGTCCAAACAGAAGCCGGGGGCCGGGGCTCGAGATCGGGGGCTGTGTCCACTGC
  AATCTGGAAAGACGAAAAACCCAACAAACCGAAAGGGGATAGGTAGGTGACAAGTGGACG
  GGAAAGGAGTGTGGGGCCTGGGCCTTTGGGCCCATCCCCCCACCCTGGGCCTGGCGGGGA
  GTGCCGGCGTCTTGCTGCCGGGGAGGCCCAGTCCTGGTGGGGGAGGCTCAGCGCTTGGCC
  CTCTCCTTAGCTGTGTGGCGCAAGCCCTCGGCCCAGGGGAAGGTCGACCCCCGGGGACAG
  GGGTTGCTCTTTGGCCTCCAGTGCTGCGCGGGGGGGGGCCCCATCTATCCTCCCCTCACC
  TCCATACATCTTCTTCTCTTCTTTTCCCTCCTTCCCCCCTCATTCCCTTCCCCCCCCCCG
  TTCCTTCTCTCTCTCCCCCTCCTCCCCTCCTCCACCATGTCATTACTCTTCCCCCTCGCT
  TCCACTCGTTTCGNTATCCCTCCTTCCTTATCTCTTCCCCACTCCCTTTCTTCCCGCCCG
  TTTTCTTTCCTCTTTCCCACTTCCCCGCTCCATCCCTTCTACTACACCCCTCTTCTTCAT
  ACCTTCGTCGCCCTCCTCTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_032281
• Insert Size: 1500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032281.1, NP_115657.1
• RefSeq Size: 4661 bp
• RefSeq ORF: 705 bp
• Locus ID: 1995
• Cytogenetics: 19p13.2
• Gene Summary: 'A member of the ELAVL protein family, ELAV-like 3 is a neural-specific RNA-binding protein which contains three RNP-type RNA recognition motifs. The observation that ELAVL3 is one of several Hu antigens (neuronal-specific RNA-binding proteins) recognized by the anti-Hu serum antibody present in sera from patients with paraneoplastic encephalomyelitis and sensory neuronopathy (PEM/PSN) suggests it has a role in neurogenesis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) lacks an in-frame segment in the coding region, as compared to variant 1. It encodes isoform 2 which lacks an internal segment, as compared to isoform 1.