HuC (ELAVL3) (NM_032281) Human Untagged Clone

CAT#: SC317571

ELAVL3 (untagged)-Human ELAV (embryonic lethal, abnormal vision, Drosophila)-like 3 (Hu antigen C) (ELAVL3), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ELAVL3
• Synonyms: HUC; HUCL; PLE21
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_032281, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGTCACTCAGATACTGGGGGCCATGGAGTCTCAGGTGGGGGGGGGCCCGGCCGGCCCGGCCCTGCCCA
  ACGGGCCACTCCTTGGTACAAATGGAGCCACTGACGACAGCAAGACCAACCTCATCGTCAACTACCTGCC
  CCAGAACATGACCCAGGATGAGTTCAAGAGTCTCTTCGGCAGCATTGGCGACATCGAGTCCTGCAAGTTG
  GTTCGGGACAAGATCACAGGGCAGAGCCTTGGCTACGGGTTTGTGAACTATTCTGACCCCAATGATGCAG
  ACAAAGCCATCAACACCCTCAACGGCCTCAAATTACAGACGAAGACCATCAAGGTGTCCTATGCCAGACC
  CAGTTCAGCATCCATCCGGGATGCTAACCTGTACGTCAGCGGGCTCCCCAAGACCATGAGCCAGAAAGAG
  ATGGAGCAGCTCTTCTCCCAGTACGGCCGCATCATCACGTCCCGCATCCTGGTGGACCAGGTCACAGGTG
  TCTCTCGGGGTGTGGGATTCATCCGCTTTGACAAGAGGATTGAGGCCGAAGAGGCTATCAAAGGACTGAA
  TGGGCAGAAGCCGCTGGGCGCAGCTGAGCCCATCACAGTCAAGTTCGCGAACAACCCAAGTCAGAAGACG
  GGGCAGGCGCTGCTCACCCACCTCTACCAGTCATCCGCCCGGCGCTACGCAGGCCCCCTACACCATCAGA
  CCCAGCGTTTCCGGCTGGACAATTTGCTCAACATGGCCTACGGCGTCAAGAGGTTCTCGCCGATCGCCAT
  CGATGGTATGAGCGGCCTGGCGGGCGTGGGCCTGTCGGGGGGCGCGGCGGGCGCCGGCTGGTGCATCTTC
  GTGTACAACCTGTCACCGGAGGCAGACGAGAGCGTGCTGTGGCAGCTGTTCGGGCCTTTTGGGGCAGTCA
  CCAACGTCAAGGTCATCCGTGATTTCACCACCAACAAGTGCAAGGGTTTCGGCTTCGTGACCATGACCAA
  CTATGACGAGGCGGCCATGGCCATCGCCAGCCTGAACGGCTATCGCCTGGGCGAGCGCGTGCTGCAGGTC
  TCCTTCAAGACCAGCAAACAGCACAAGGCGTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_032281
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032281.2, NP_115657.2
• RefSeq Size: 4661 bp
• RefSeq ORF: 1083 bp
• Locus ID: 1995
• Cytogenetics: 19p13.2
• Gene Summary: 'A member of the ELAVL protein family, ELAV-like 3 is a neural-specific RNA-binding protein which contains three RNP-type RNA recognition motifs. The observation that ELAVL3 is one of several Hu antigens (neuronal-specific RNA-binding proteins) recognized by the anti-Hu serum antibody present in sera from patients with paraneoplastic encephalomyelitis and sensory neuronopathy (PEM/PSN) suggests it has a role in neurogenesis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) lacks an in-frame segment in the coding region, as compared to variant 1. It encodes isoform 2 which lacks an internal segment, as compared to isoform 1.