Glutaredoxin 1 (GLRX) (BC010965) Human Untagged Clone

CAT#: SC105918

GLRX (untagged)-Homo sapiens, Similar to glutaredoxin (thioltransferase), clone MGC:13682 IMAGE:4333310, complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GLRX
• Synonyms: glutaredoxin (thioltransferase); GRX; GRX1; MGC117407; OTTHUMP00000149979
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC105918 sequence for BC010965 edited (data generated by NextGen Sequencing)
  ATGGCTCAAGAGTTTGTGAACTGCAAAATCCAGCCTGGGAAGGTGGTTGTGTTCATCAAG
  CCCACCTGCCCGTACTGCAGGAGGGCCCAAGAGATCCTCAGTCAATTGCCCATCAAACAA
  GGGCTTCTGGAATTTGTCGATATCACAGCCACCAACCACACTAACGAGATTCAAGATTAT
  TTGCAACAGCTCACGGGAGCAAGAACGGTGCCTCGAGTCTTTATCGGTAAAGATTGTATA
  GGCGGATGCAGTGATCTAGTCTCTTTGCAACAGAGTGGGGAACTGCTGACGCGGCTAAAG
  CAGATTGGAGCTCTGCAGTAA
  
  Clone variation with respect to BC010965.1
  225 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC010965 unedited
  ACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGCCTCGTGCCGAATTCGGCACGAG
  GGCAATACCTGCAACTGAGGATTCTTCCCGGGGAGACCGCAGCCCATCGGCATGGCTCAA
  GAGTTTGTGAACTGCAAAATCCAGCCTGGGAAGGTGGTTGTGTTCATCAAGCCCACCTGC
  CCGTACTGCAGGAGGGCCCAAGAGATCCTCAGTCAATTGCCCATCAAACAAGGGCTTCTG
  GAATTTGTCGATATCACAGCCACCAACCACACTAACGAGATTCAAGATTATTTGCAACAG
  CTCACGGGAGCAAGAACGGTGCCTCGAGTCTTTATCGGTAAAGATTGTATAGGCGGATGC
  AGTGATCTAGTCTCTTTGCAACAGAGTGGGGAACTGCTGACGCGGCTAAAGCAGATTGGA
  GCTCTGCAGTAACCACAGATCTCATAGGAAATGTTCAACAATTCTGTGAAAGGTCACAGG
  ACCCAATTGGAGAAATCATATGAAAAGCATAGTTGGTCTTGGTGTCATATGGATCAGAGG
  CANCAGTGCAGAGGCTGTGGTCATGCGGAACACTCTGTTATTTAAGATGGCTATCCAGAT
  AATCCTGAACACTGTGTATTTATTTTATTTAGACTACCAGCAAAGATTAAAGCATGAAAT
  GTAAAACATCTGATAAAACTTACAGCCCCCTACACCAAAGTGTATCTGTGAAAAGCTCCT
  ACACTTTGAAAACTAAGAATCCCTTACATGAAGTTGCCTGTTCTANAATTGAAGAATGTA
  ATTTCCCTCATCTCTAGGACACACTTAATTTCTTTTCTATAAAAAAACCCTTTGATGAAA
  AAAAAAAAAAAACTCTATTTATATGGCGGCCGCGTCATAGTTGTTTCTGAAAAAACCCGG
  TTGGGATCCTGTGACCCCTCCCATGCTTTTCTGCCCTGAAATGCCCCTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for BC010965 unedited
  GGCCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCATCTATAGGTTTTTT
  TTATTAAAAAAAAAATTAAGTGTTGTCACTAGAGATTGAAGGAAATTAACAATCTTACAA
  TTCTAGAACAGGCAAACTTCATGATAAGGGATTCTTAAGTTTTCAAAGTGTAGGAGCTCT
  TTCACAGATACACTCTTGGTGTAGGGGGCTGTAAGTTTTATCAGATGTTTTACATTTCAT
  GCTTTAATCTTTGCTGGTAGTCTAAATAAAATAAATACACAGTGTTCAGGATTATCTGGA
  TAGCCATCTTAAATAACAGAGTGTTCCGCATGACCACAGCCTCTGCACTTGTGCCTCTGA
  TCCATATGACACCAAGACCAACTATGCTTTTCATATGATTTCTCCAATTGGGTCCTGTGA
  CCTTTCACAGAATTGTTGAACATTTCCTATGAGATCTGTGGTTACTGCAGAGCTCCAATC
  TGCTTTAGCCGCGTCAGCAGTTCCCCACTCTGTTGCAAAGAGACTAGATCACTGCATCCG
  CCTATACAATCTTTACCGATAAAGACTCGAGGCACCGTTCTTGCTCCCGTGAGCTGTTGC
  AAATAATCTTGAATCTCGTTAGTGTGGTTGGTGGCTGTGATATCGACAAATTCCAGAAGC
  CCTTGTTTGATGGGCAATTGACTGAGGATCTCTTGGGCCCTCCTGCAGTACGGGCAGGTG
  GGCTTGATGAACACAACCACCTTCCCAGGCTGGATTNTGCAGTTCACAAACTCTTGAGCC
  ATGCCGATGGGCTGCNNGTCTCCCCGGGAAGAATCCTCAGTTGCAGGTATTGCCCTCGTG
  CCGAAATCGCACGAGGCTCGTGCCGAAATCCCGGCCGCCCTATAGTGAGTCGTATTACAA
  AATCTGACGGTTACTTAACGAGCCTGCTTATTAGACCTCA
  
• Restriction Sites: NotI-NotI
• ACCN: BC010965
• Insert Size: 850 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC010965.1, AAH10965.1
• RefSeq Size: 836 bp
• RefSeq ORF: 321 bp
• Locus ID: 2745
• Cytogenetics: 5q15
• Domains: glutaredoxin
• Protein Families: Druggable Genome
• Gene Summary: 'This gene encodes a member of the glutaredoxin family. The encoded protein is a cytoplasmic enzyme catalyzing the reversible reduction of glutathione-protein mixed disulfides. This enzyme highly contributes to the antioxidant defense system. It is crucial for several signalling pathways by controlling the S-glutathionylation status of signalling mediators. It is involved in beta-amyloid toxicity and Alzheimer's disease. Multiple alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq, Aug 2011]'