NQO1 (BC000906) Human Untagged Clone

CAT#: SC105922

(untagged)-Homo sapiens, Similar to Diaphorase (NADH/NADPH), clone IMAGE:2901105

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NQO1
• Synonyms: DHQU|DIA4|DTD|NMOR1|NMORI|QR1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for BC000906 edited
  GAATTCGGCACGAGGACCCCAGGATTCAGGCGTTGGGTCCCGCCCTTGTAGGCTGTCCAC
  CTCAAACGGGCCGGACAGGATATATAAGAGAGAATGCACCGTGCACTACACACGCGACTC
  CCACAAGGTTGCAGCCGGAGCCGCCCAGCTCACCGAGAGCCTAGTTCCGGCCAGGGTCGC
  CCCGGCAACCACGAGCCCAGCCAATCAGCGCCCCGGACTGCACCAGAGCCATGGTCGGCA
  GAAGAGCACTGATCGTACTGGCTCACTCAGAGAGGACGTCCTTCAACTATGCCATGAAGG
  AGGCTGCTGCAGCGGCTTTGAAGAAGAAAGGATGGGAGGTGGTGGAGTCGGACCTCTATG
  CCATGAACTTCAATCCCATCATTTCCAGAAAGGACATCACAGGTAAACTGAAGGACCCTG
  CGAACTTTCAGTATCCTGCCGAGTCTGTTCTGGCTTATAAAGAAGGCCATCTGAGCCCAG
  ATATTGTGGCTGAACAAAAGAAGCTGGAAGCCGCAGACCTTGTGATATTCCAGTTCCCCC
  TGCAGTGGTTTGGAGTCCCTGCCATTCTGAAAGGCTGGTTTGAGCGAGTGTTCATAGGAG
  AGTTTGCTTACACTTACGCTGCCATGTATGACAAAGGACCCTTCCGGAGTAAGAAGGCAG
  TGCTTTCCATCACCACTGGTGGCAGTGGCTCCATGTACTCTCTGCAAGGGATCCACGGGG
  ACATGAATGTCATTCTCTGGCCAATTCAGAGTGGCATTCTGCATTTCTGTGGCTTCCAAG
  TCTTAGAACCTCAACTGACATATAGCATTGGGCACACTCCAGCAGACGCCCGAATTCAAA
  TCCTGGAAGGATGGAAGAAACGCCTGGAGAATATTTGGGATGAGACACCACTGTATTTTG
  CTCCAAGCAGCCTCTTTGACCTAAACTTCCAGGCAGGATTCTTAATGAAAAAAGAGGTAC
  AGGATGAGGAGAAAAACAAGAAATTTGGCCTTTCTGTGGGCCATCACTTGGGCAAGTCCA
  TCCCAACTGACAACCAGATCAAAGCTAGAAAATGAGATTCCTTAGCCTGGATTTCCTTCT
  AACATGTTATCAAATCTGGGTATCTTTCCAGGCTTCCCTGACTTGCTTTAGTTTTTAAGA
  TTTGTGTTTTTCTTTTTCCACAAGGAATAAATGAGAGGGAATCGACTGTATTCGTGCATT
  TTTGGATCATTTTTAACTGATTCTTATGATTACTATCATGGCATATAACCAAAATCCGAC
  TGGGCTCAAGAGGCCACTTAGGGAAAGATGTAGAAAGATGCTAGAAAAATGTTCTTTAAA
  GGCATCTACACAATTTAATTCCTCTTTTTAGGGCTAAAGTTTTAGGGTACAGTTTGGCTA
  GGTATCATTCAACTCTCCAATGTTCTATTAATCACCTCTCTGTAGTTTATGGCAGAAGGG
  AATTGCTCAGAGAAGGAAAAGACTGAATCTACCTGCCCTAAGGGACTTAACTTGTTTGGT
  AGTTAGCCATCTAATGCTTGTTTATGATATTTCTTGCTTTCAATTACAAAGCAGTTACTA
  ATATGCCTAGCACAAGTACCACTCTTGGTCAGCTTTTGTTGTTTATATACAGTACACAGA
  TACCTTGAAAGGAAGAGCTAATAAATCTCTTCTTTGCTGCAGTCATCTACTTTTTTTTTA
  ATTAAAAAAAATTTTTTTTTGAAGCAGTCTTGCTCTGTTACCCAGGCTGGAGTGCAGTGG
  TGTGATCTCGGCTCACTGCAACCTCTGCCTCCCAGGTTCCAGCAATTCTCCTGCCTCAGC
  CTCCCTAGTAGCTGGGATGACAGGCGCCTGCCATCATGCCTGACTAATTTTTGTATTTTT
  AGTAGAGACGGCGTTTCACCATGTTGGCCAGGCTGGTCTCAAACTCCTGACCTCAGGTGA
  TCCGCCTACCTCAGCCTCCCAAAGTGCTGGGATTACAGGCGTGATCCACCACACCTGGCC
  CTTGCAATCTTCTACTTTAAGGTTTGCAGAGATAAACCAATAAATCCACACCGTACATCT
  GCAATATGAATTCAAGAAAGGAAATAGTACCTTCAATACTTAAAAATAGTCTTCCACAAA
  AAATACTTTATTTCTGATCTATACAAATTTTCAGAAGGTTATTTTCTTTATCATTGCTAA
  ACTGATGACTTACCATGGGATGGGGTCCAGTCCCATGACCTTGGGGTACAATTGTAAACC
  TAGAGTTTTATCAACTTTGGTGAACAGTTTTGGCATAATAGTCAATTTCTACTTCTGGAA
  GTCATCTCATTCCACTGTTGGTATTATATAATTCAAGGAGAATATGATAAAACACTGCCC
  TCTTGTGGTGCATTGAAAGAAGAGATGAGAAATGATGAAAAGGTTGCCTGAAAAATGGGA
  GACAGCCTCTTACTTGCCAAGAAAATGAAGGGATTGGACCGAGCTGGAAAACCTCCTTTA
  CCAGATGCTGACTGGCACTGGTGGTTTTTGCTCTCGACAGTATCCACAATAGCTGACGGC
  TGGGTGTTTCAGTTTGAAAATATTTTGTTGCCTTCATCTTCACTGCAATTTTGTGTAAAT
  TTCTCAAAGATCTGAATTAAATAAATAAAATTCATTTCTACAGACCCACAAAAAAAAAAA
  AAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC000906 unedited
  AATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGACCCCAGNATTCAGGCGTT
  GGGTCCCGCCCTTGTAGGCTGTCCACCTCAAACGGGCCGGACAGGATATATAAGAGAGAA
  TGCACCGTGCACTACACACGCGACTCCCACAAGGTTGCAGCCGGAGCCGCCCAGCTCACC
  GAGAGCCTAGTTCCGGCCAGGGTCGCCCCGGCAACCACGAGCCCAGCCAATCAGCGCCCC
  GGACTGCACCAGAGCCATGGTCGGCAGAAGAGCACTGATCGTACTGGCTCACTCAGAGAG
  GACGTCCTTCAACTATGCCATGAAGGAGGCTGCTGCAGCGGCTTTGAAGAAGAAAGGATG
  GGAGGTGGTGGAGTCGGACCTCTATGCCATGAACTTCAATCCCATCATTTCCAGAAAGGA
  CATCACAGGTAAACTGAAGGACCCTGCGAACTTTCAGTATCCTGCCGAGTCTGTTCTGGC
  TTATAAAGAAGGCCATCTGAGCCCAGATATTGTGGCTGAACAAAAGAAGCTGGAAGCCGC
  AGACCTTGTGATATTCCAGTTCCCCCTGCAGTGGTTTGGAGTCCCTGCCATTCTGAAAGG
  CTGGTTTGAGCGAGTGTTCATAGGAGAGTTTGCTTACACTTACGCTGCCATGTATGACAA
  AGGACCCTTCCGGAGTAAGAAAGCAGTGCTTTCCATCACCACTGGTGGCAGTGGCTCCAT
  GTACTCTCTGCNAGGGGATCCACGGGACCATGAATGTCATTCTCTGGCCCATTCAGAAGT
  GCATTCTGCAATTTCTGTGGCTTNCCAGTCTTANAACCCTCACTGACATATAGCATTGGG
  CACACTNCAGCAGACGGCCGATTCAATCCTGNNAGGNATGGAGAAACGCTGNAGAATATT
  TGGGATGAGACACACTGTATTNTTGCTCAGCAGNCTCTTTGACTAACTNNCAGCNGATCT
  ATGNAAAAAAAGGCCGGGTGTAGAGAAACAGAAA
  
• Restriction Sites: NotI-NotI
• ACCN: BC000906
• Insert Size: 2700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC000906.2
• RefSeq Size: 974 bp
• RefSeq ORF: 974 bp
• Locus ID: 1728
• Cytogenetics: 16q22.1
• Protein Families: Druggable Genome
• Gene Summary: 'This gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]'