CKII alpha (CSNK2A1) (NM_177559) Human Untagged Clone

CAT#: SC107027

CSNK2A1 (untagged)-Human casein kinase 2, alpha 1 polypeptide (CSNK2A1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CSNK2A1
• Synonyms: CK2A1; Cka1; Cka2; CKII; OCNDS
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_177559 edited
  ATGTCGGGACCCGTGCCAAGCAGGGCCAGAGTTTACACAGATGTTAATACACACAGACCT
  CGAGAATACTGGGATTACGAGTCACATGTGGTGGAATGGGGAAATCAAGATGACTACCAG
  CTGGTTCGAAAATTAGGCCGAGGTAAATACAGTGAAGTATTTGAAGCCATCAACATCACA
  AATAATGAAAAAGTTGTTGTTAAAATTCTCAAGCCAGTAAAAAAGAAGAAAATTAAGCGT
  GAAATAAAGATTTTGGAGAATTTGAGAGGAGGTCCCAACATCATCACACTGGCAGACATT
  GTAAAAGACCCTGTGTCACGAACCCCCGCCTTGGTTTTTGAACACGTAAACAACACAGAC
  TTCAAGCAATTGTACCAGACGTTAACAGACTATGATATTCGATTTTACATGTATGAGATT
  CTGAAGGCCCTGGATTATTGTCACAGCATGGGAATTATGCACAGAGATGTCAAGCCCCAT
  AATGTCATGATTGATCATGAGCACAGAAAGCTACGACTAATAGACTGGGGTTTGGCTGAG
  TTTTATCATCCTGGCCAAGAATATAATGTCCGAGTTGCTTCCCGATACTTCAAAGGTCCT
  GAGCTACTTGTAGACTATCAGATGTACGATTATAGTTTGGATATGTGGAGTTTGGGTTGT
  ATGCTGGCAAGTATGATCTTTCGGAAGGAGCCATTTTTCCATGGACATGACAATTATGAT
  CAGTTGGTGAGGATAGCCAAGGTTCTGGGGACAGAAGATTTATATGACTATATTGACAAA
  TACAACATTGAATTAGATCCACGTTTCAATGATATCTTGGGCAGACACTCTCGAAAGCGA
  TGGGAACGCTTTGTCCACAGTGAAAATCAGCACCTTGTCAGCCCTGAGGCCTTGGATTTC
  CTGGACAAACTGCTGCGATATGACCACCAGTCACGGCTTACTGCAAGAGAGGCAATGGAG
  CACCCCTATTTCTACACTGTTGTGAAGGACCAGGCTCGAATGGGTTCATCTAGCATGCCA
  GGGGGCAGTACGCCCGTCAGCAGCGCCAATATGATGTCAGGGATTTCTTCAGTGCCAACC
  CCTTCACCCCTTGGACCTCTGGCAGGCTCACCAGTGATTGCTGCTGCCAACCCCCTTGGG
  ATGCCTGTTCCAGCTGCCGCTGGCGCTCAGCAGTAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_177559 unedited
  TGTAATACGACTCACTATAGGGCGGCCGCGCAATTCGCACGAGGGTCTCCCCCCTCCATC
  GCCGCCATATTGTCTGTGTGAGCAGAGGGGAGAGCGGCCGCCGCCGCTGCCGCTTCCACC
  ACAGCTCTATCAAGGCTTGTCAAGCAGTGTGCTCATCACATCGTAAATCATGCAGCGTGG
  AACCTCATAAAATCTCCAACAAACATCATTCACCCATACTGACTAGTTTCACATCTCTTT
  GTTTGAAGAAAACAGGTCTGAAACAAGGTCTTACCCCCAGCTGCTTCTGAACACAGTGAC
  TGCCAGATCTCCAAACATCAAGTCCAGCTTTGTCCGCCAACCTGTCTGACATGTCGGGAC
  CCGTGCCAAGCAGGGCCAGAGTTTACACAGATGTTAATACACACAGACCTCGAGAATACC
  TGGGATTACGAGTCACATGTGGTGGAATGGGGAAATCAAGATGACTACCAGCTGGTTCGA
  AAATTACGCCGAGGTAAATACAGTGAAGTATTTGAAGCCATCAACATCACAAATAATGAA
  AAAGTTGTTGCTAAAATTCTCAAGCCAGCAAAAAAGAACAAATTAAGCGTGAAATAAAGA
  TTTTGGAGAATTTGAGAGGAGGTCCCACCATCATCACACTGGCAGACATGTAAAAGACCC
  TGTGTCACGAACCCCCGCCTTGGTTTTGAACACCAAACACACAGACTTAAGCAATTGTAC
  CAGACGTCTACAGACTATGAATATCCGATCTTACATGTATGACATTCCGAAAGCCCTTGA
  ATATTGTCCCAGCATGGGAATTATGCACAGAGATGCCAGCCCCATAAAGTCATGATTGAC
  CCGGACCCCAAAAGCCTCACCAATAGAACCGCGTTTGGCTGAGTTTTATCATCCGGCCAA
  CAATAAAGCCCGCAGTGCCTCCGAACCTTAAGGCCCGGGCTTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_177559 unedited
  CTGTTACCGCGCCCGCATTCTANATCGAGTTTTTTCTTTTTTTTTTTCACTATGAAGGAC
  AAAGTATTATATATATACACACAGCAAGGGGTGGCGGGGCTGTAACAAGAGAGTTTATAG
  TTTTCCCACAATTACAGGTCTACCATTTCAGCTTCAATGGAGATAGTGGCTCTGCTCCTA
  CCTCTCAAGATACATTTACAAGACTGAGGATCAGGTCTTCTCACTGGATGGCATGTGAGG
  GAAGGGGACGGTCGGAGGAAAAGAAGGTAGAAAGCTTAAAATGGAAGTCAGTGTGGCCAC
  ATCTCCCATTAGCTCTAGCATGAAACCTGTACAGACAATGTTTGTTTCTTTTGTAAAAAG
  CAGTAAGTTATGCCCAGTAACTAAATGAATTCAAAATGGCCAAGACAAAGAAAACTAAGA
  AAGATTTTGCCTTCCCTCTCCTACCAGCTATGGAGCACAGCATGTTGGGAGATGAACAGG
  GAAAAGACCAAGGTAAGGAGCCTGNGAGGGAAGGTATCAACATTTTAAACTGAACTAAAA
  ATAAAAGTATAAATGAGTTGGATTTAGGGTTAGATCAGTAAGACATGATTCTTACTGAAC
  AGAAGTTTTTAGTATCTGTCTGCATTTTGGGTAGATTTTCAACATCTTGATGTAACTAAG
  ACACACTTCCAACAGAGCCACTAGGATAACCCCACTGAAGCGCTTATGGAGTAAAGTGAT
  GTAAGCGACCAGNCAGCAGTCCACTGCTCCTATAGATTGGGTCTNCCCTTCCTCTTNNCT
  TCCTTCCTTNCTTTCTGGCTCCCCTGAGAAACTACATTTTTGTATTCTCTGGAGAAGACC
  CCGAAATACCAACCCCTTAAGTGTGGGACCGTGGAAGAAACATGTGTGGAATGGGAAGAA
  TTCCCCACTAAATGCACCACCCCGGATTGAGGCTGGTTACCTTTGTCCTTTTTGATAAGA
  A
  
• Restriction Sites: NotI-NotI
• ACCN: NM_177559
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177559.2, NP_808227.1
• RefSeq Size: 2849 bp
• RefSeq ORF: 1176 bp
• Locus ID: 1457
• Cytogenetics: 20p13
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase
• Protein Pathways: Adherens junction, Tight junction, Wnt signaling pathway
• Gene Summary: 'Casein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. It is involved in various cellular processes, including cell cycle control, apoptosis, and circadian rhythm. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The protein encoded by this gene represents the alpha subunit. Multiple transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq, Apr 2018]'
  Transcript Variant: This variant (1) encodes the longer isoform (a). Variants 1, 2, 4 and 5 encode the same protein.