CKII alpha (CSNK2A1) (NM_177559) Human Untagged Clone

CAT#: SC323516

CSNK2A1 (untagged)-Kinase deficient mutant (K68M) of Human casein kinase 2, alpha 1 polypeptide (CSNK2A1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CSNK2A1
• Synonyms: CK2A1; Cka1; Cka2; CKII; OCNDS
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_177559, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTCGGGACCCGTGCCAAGCAGGGCCAGAGTTTACACAGATGTTAATACACACAGACCTCGAGAATACT
  GGGATTACGAGTCACATGTGGTGGAATGGGGAAATCAAGATGACTACCAGCTGGTTCGAAAATTAGGCCG
  AGGTAAATACAGTGAAGTATTTGAAGCCATCAACATCACAAATAATGAAAAAGTTGTTGTTAAAATTCTC
  AAGCCAGTAAAAAAGAAGAAAATTAAGCGTGAAATAAAGATTTTGGAGAATTTGAGAGGAGGTCCCAACA
  TCATCACACTGGCAGACATTGTAAAAGACCCTGTGTCACGAACCCCCGCCTTGGTTTTTGAACACGTAAA
  CAACACAGACTTCAAGCAATTGTACCAGACGTTAACAGACTATGATATTCGATTTTACATGTATGAGATT
  CTGAAGGCCCTGGATTATTGTCACAGCATGGGAATTATGCACAGAGATGTCAAGCCCCATAATGTCATGA
  TTGATCATGAGCACAGAAAGCTACGACTAATAGACTGGGGTTTGGCTGAGTTTTATCATCCTGGCCAAGA
  ATATAATGTCCGAGTTGCTTCCCGATACTTCAAAGGTCCTGAGCTACTTGTAGACTATCAGATGTACGAT
  TATAGTTTGGATATGTGGAGTTTGGGTTGTATGCTGGCAAGTATGATCTTTCGGAAGGAGCCATTTTTCC
  ATGGACATGACAATTATGATCAGTTGGTGAGGATAGCCAAGGTTCTGGGGACAGAAGATTTATATGACTA
  TATTGACAAATACAACATTGAATTAGATCCACGTTTCAATGATATCTTGGGCAGACACTCTCGAAAGCGA
  TGGGAACGCTTTGTCCACAGTGAAAATCAGCACCTTGTCAGCCCTGAGGCCTTGGATTTCCTGGACAAAC
  TGCTGCGATATGACCACCAGTCACGGCTTACTGCAAGAGAGGCAATGGAGCACCCCTATTTCTACACTGT
  TGTGAAGGACCAGGCTCGAATGGGTTCATCTAGCATGCCAGGGGGCAGTACGCCCGTCAGCAGCGCCAAT
  ATGATGTCAGGGATTTCTTCAGTGCCAACCCCTTCACCCCTTGGACCTCTGGCAGGCTCACCAGTGATTG
  CTGCTGCCAACCCCCTTGGGATGCCTGTTCCAGCTGCCGCTGGCGCTCAGCAGTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_177559 unedited
  ACGCCCGTAGAGCAATGGGCGGTAGGCGCTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA
  ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTCTCCTCCTCCA
  TCGCCGCCATATTGTCTGTGTGAGCAGAGGGGAGAGCGGCCGCCGCCGCTGCCGCTTCCACCACAGCTCT
  ATCAAGGCTTGTCAAGCAGTGTGCTCATCACATGGTAAATCATGCAGCGTGGAACCTCATAAAATCTCCA
  AGAAACATCATTCACCCATACTGACTAGTTTCACATCTCTTTGTTTGAAGAAAAACAGGTCTGAAAACAA
  GGTCTAACCCCCAGCTGCTTCTGAACACAGTGACTGCCAGAATCTCCAAACATCAAGTCCCAGCTTTGTC
  CGCCAACCGGTCTGACTGGTCGGAACCCGTGCAAGCAGGGCCAGAGTTTACCCAGTGTTAATCCCCCACC
  CTCGAAAACCTGGAATACAGTTCCAGGTGTGGATTGGGAAATCAAAGGATACACTGGTTCAAATTAGCAG
  AGTACTCGTGGATTTTTGACCCCCCAAACACATAAAAA
  
  Kinase domain sequence

  >SC323516 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  TGATCGAATTATGCCGAGKAATACAGTGAAGTATTTGAAGCCATCAACATCACAAATAATGAAAAAGTTG
  TTGTTATGATTCTCAAGCCAGTAAAAAAGAAGAAAATTAAGCGTGAAATAAAGATTTTGGAGAATTTGAG
  AGGAGGTCCCAACATCATCACACTGGCAGACATTGTAAAAGACCCTGTGTCACGAACCCCCGCCTTGGTT
  TTTGAACACGTAAACAACACAGACTTCAAGCAATTGTACCAGACG
  
• Restriction Sites: Please inquire
• ACCN: NM_177559
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177559.2, NP_808227.1
• RefSeq Size: 2849 bp
• RefSeq ORF: 1176 bp
• Locus ID: 1457
• Cytogenetics: 20p13
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase
• Protein Pathways: Adherens junction, Tight junction, Wnt signaling pathway
• Gene Summary: 'Casein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. It is involved in various cellular processes, including cell cycle control, apoptosis, and circadian rhythm. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The protein encoded by this gene represents the alpha subunit. Multiple transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq, Apr 2018]'
  Transcript Variant: This variant (1) encodes the longer isoform (a). Variants 1, 2, 4 and 5 encode the same protein.