TFIIS (TCEA2) (NM_198723) Human Untagged Clone

CAT#: SC107876

TCEA2 (untagged)-Human transcription elongation factor A (SII), 2 (TCEA2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TCEA2
• Synonyms: TFIIS
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_198723, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGATTTGCTGCGGGAGCTGAAGGCCATGCCTATCACGCTGCACCTGCTCCAGTCCACCCGAGTCGGGA
  TGTCTGTCAACGCCCTTCGGAAGCAGAGCTCGGATGAGGAGGTCATTGCACTGGCCAAGTCTCTCATCAA
  GTCCTGGAAGAAGCTCCTGGATGCTTCCGATGCCAAAGCCAGGGAGCGGGGGAGGGGCATGCCTCTGCCC
  ACGTCCTCGAGGGATGCCTCAGAGGCCCCGGATCCCAGCCGCAAGAGGCCGGAGCTGCCCAGGGCACCGT
  CGACTCCGAGGATCACCACATTTCCTCCGGTGCCTGTCACCTGTGATGCCGTGCGCAACAAGTGCCGCGA
  GATGCTGACCGCTGCCCTGCAGACGGACCATGACCACGTGGCCATCGGTGCGGACTGCGAGCGCCTGTCG
  GCTCAGATCGAGGAATGCATCTTCCGGGACGTTGGAAACACAGACATGAAGTATAAGAACCGTGTACGGA
  GTCGTATCTCCAACCTGAAGGATGCCAAGAACCCTGACCTGCGGCGGAATGTGCTGTGTGGGGCCATAAC
  ACCCCAGCAGATCGCTGTGATGACCTCAGAGGAGATGGCCAGTGATGAGCTGAAGGAGATCCGTAAGGCC
  ATGACCAAGGAGGCCATCCGAGAGCACCAGATGGCCCGCACTGGCGGCACGCAGACAGACCTGTTCACCT
  GCGGCAAGTGCAGGAAAAAGAACTGCACCTACACACAGGTGCAGACCCGCAGCTCTGATGAGCCCATGAC
  CACCTTTGTTGTCTGCAACGAGTGTGGAAACCGCTGGAAGTTCTGCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_198723 unedited
  NNTTGTCAGATTTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACCAGCCCTGCT
  GTGGTGCAGGAACATGTGGGCAGAGCCCTGGGTGTCTCCAGGAGGGAGCCATGGATTTGC
  TGCGGGAGCTGAAGGCCATGCCTATCACGCTGCACCTGCTCCAGTCCACCCGAGTCGGGA
  TGTCTGTCAACGCCCTTCGGAAGCAGAGCTCGGATGAGGAGGTCATTGCACTGGCCAAGT
  CTCTCATCAAGTCCTGGAAGAAGCTCCTGGATGCTTCCGATGCCAAAGCCAGGGAGCGGG
  GGAGGGGCATGCCTCTGCCCACGTCCTCGAGGGATGCCTCAGAGGCCCCGGATCCCAGCC
  GCAAGAGGCCGGAGCTGCCCAGGGCACCGTCGACTCCGAGGATCACCACATTTCCTCCGG
  TGCCTGTCACCTGTGATGCCGTGCGCAACAAGTGCCGCGAGATGCTGACCGCTGCCCTGC
  AGACGGACCATGACCACGTGGCCATCGGTGCGGACTGCGAGCGCCTGTCGGCTCAGATCG
  AGGAATGCATCTTCCGGGACGTTGGAAACACAGACATGAAGTATAAGAACCGTGTACGGA
  GTCGTATCTCCAACCTGAAGGATGCCAAGAACCCTGACCTGCGGCGGAATGTGCTGTGTG
  GGGCCATAACACCCCAGCAGATCGCTGTGATGACCTCAGAGGAGATGGCCAGTGATGAGC
  TGAANGGAGATCCGTAGGCCATGACCAAGGAGGCCATCCGAGAGCACCAGATGGCCCGCA
  CTGGCGGCACGCAGACAGACCTGTTCACCTGCNGCAAGTGCAGGAAAAAGAACTGCACCT
  ACACACAGGTGCAGACCCGCAGCTCTGATGAGCCATGACACCTTTTGNTGTCTGCACGAG
  TGTGGAAACGCTGGAAGTCTGCTCACCCTCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_198723
• Insert Size: 2900 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198723.1, NP_942016.1
• RefSeq Size: 1661 bp
• RefSeq ORF: 819 bp
• Locus ID: 6919
• Cytogenetics: 20q13.33
• Protein Families: Transcription Factors
• Gene Summary: 'The protein encoded by this gene is found in the nucleus, where it functions as an SII class transcription elongation factor. Elongation factors in this class are responsible for releasing RNA polymerase II ternary complexes from transcriptional arrest at template-encoded arresting sites. The encoded protein has been shown to interact with general transcription factor IIB, a basal transcription factor. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) differs in the 5' UTR and coding region compared to variant 1. The resulting isoform (b) is shorter at the N-terminus compared to isoform a.