TFIIS (TCEA2) (NM_198723) Human Untagged Clone

CAT#: SC320353

TCEA2 (untagged)-Human transcription elongation factor A (SII), 2 (TCEA2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TCEA2
• Synonyms: TFIIS
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_198723.1 GTCGGCTGCGGCGGGTGTGGGAGGTGGCGACGGCCGGGGCCGGGGTCCTGCCCGGCTGCG
  GAGGCGGGCGCGACGGGCGCGGGGGTCGCTGCTCCTGAGGCGATGATGGGCAAGGAAGAG
  GAGATTGCGCGGATCGCCCGGAGGCTGGACAAGATGGTGACCAAGAAGAGCGCGGAGGGA
  GCCATGGATTTGCTGCGGGAGCTGAAGGCCATGCCTATCACGCTGCACCTGCTCCAGTCC
  ACCCGAGTCGGGATGTCTGTCAACGCCCTTCGGAAGCAGAGCTCGGATGAGGAGGTCATT
  GCACTGGCCAAGTCTCTCATCAAGTCCTGGAAGAAGCTCCTGGATGCTTCCGATGCCAAA
  GCCAGGGAGCGGGGGAGGGGCATGCCTCTGCCCACGTCCTCGAGGGATGCCTCAGAGGCC
  CCGGATCCCAGCCGCAAGAGGCCGGAGCTGCCCAGGGCACCGTCGACTCCGAGGATCACC
  ACATTTCCTCCGGTGCCTGTCACCTGTGATGCCGTGCGCAACAAGTGCCGCGAGATGCTG
  ACCGCTGCCCTGCAGACGGACCATGACCACGTGGCCATCGGTGCGGACTGCGAGCGCCTG
  TCGGCTCAGATCGAGGAATGCATCTTCCGGGACGTTGGAAACACAGACATGAAGTATAAG
  AACCGTGTACGGAGTCGTATCTCCAACCTGAAGGATGCCAAGAACCCTGACCTGCGGCGG
  AATGTGCTGTGTGGGGCCATAACACCCCAGCAGATCGCTGTGATGACCTCAGAGGAGATG
  GCCAGTGATGAGCTGAAGGAGATCCGTAAGGCCATGACCAAGGAGGCCATCCGAGAGCAC
  CAGATGGCCCGCACTGGCGGCACGCAGACAGACCTGTTCACCTGCGGCAAGTGCAGGAAA
  AAGAACTGCACCTACACACAGGTGCAGACCCGCAGCTCTGATGAGCCCATGACCACCTTT
  GTTGTCTGCAACGAGTGTGGAAACCGCTGGAAGTTCTGCTGACCCCTCGTGTAGATGTGC
  TGCAGCCTTGGGCCCTCCCCGGCCCACGTCCTCCGTTGACACAGCTTCTCTGGAGACCCT
  AGAAGGCGGCATGTCCTGCCCTCAACCTGCCTGCCTGGATTGCACCTTTCTGCCCTTTCC
  CCCTCATTATTAAATGTTTCTTTTTGCCAAAAAAAACAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_198723
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198723.1, NP_942016.1
• RefSeq Size: 1661 bp
• RefSeq ORF: 819 bp
• Locus ID: 6919
• Cytogenetics: 20q13.33
• Protein Families: Transcription Factors
• Gene Summary: 'The protein encoded by this gene is found in the nucleus, where it functions as an SII class transcription elongation factor. Elongation factors in this class are responsible for releasing RNA polymerase II ternary complexes from transcriptional arrest at template-encoded arresting sites. The encoded protein has been shown to interact with general transcription factor IIB, a basal transcription factor. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) differs in the 5' UTR and coding region compared to variant 1. The resulting isoform (b) is shorter at the N-terminus compared to isoform a.