Carbonic Anhydrase II (CA2) (NM_000067) Human Untagged Clone

CAT#: SC107902

CA2 (untagged)-Human carbonic anhydrase II (CA2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CA2
• Synonyms: CA-II; CAC; CAII; Car2; HEL-76; HEL-S-282
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC107902 sequence for NM_000067 edited (data generated by NextGen Sequencing)
  ATGTCCCATCACTGGGGGTACGGCAAACACAACGGACCTGAGCACTGGCATAAGGACTTC
  CCCATTGCCAAGGGAGAGCGCCAGTCCCCTGTTGACATCGACACTCATACAGCCAAGTAT
  GACCCTTCCCTGAAGCCCCTGTCTGTTTCCTATGATCAAGCAACTTCCCTGAGGATCCTC
  AACAATGGTCATGCTTTCAACGTGGAGTTTGATGACTCTCAGGACAAAGCAGTGCTCAAG
  GGAGGACCCCTGGATGGCACTTACAGATTGATTCAGTTTCACTTTCACTGGGGTTCACTT
  GATGGACAAGGTTCAGAGCATACTGTGGATAAAAAGAAATATGCTGCAGAACTTCACTTG
  GTTCACTGGAACACCAAATATGGGGATTTTGGGAAAGCTGTGCAGCAACCTGATGGACTG
  GCCGTTCTAGGTATTTTTTTGAAGGTTGGCAGCGCTAAACCGGGCCTTCAGAAAGTTGTT
  GATGTGCTGGATTCCATTAAAACAAAGGGCAAGAGTGCTGACTTCACTAACTTCGATCCT
  CGTGGCCTCCTTCCTGAATCCCTGGATTACTGGACCTACCCAGGCTCACTGACCACCCCT
  CCTCTTCTGGAATGTGTGACCTGGATTGTGCTCAAGGAACCCATCAGCGTCAGCAGCGAG
  CAGGTGTTGAAATTCCGTAAACTTAACTTCAATGGGGAGGGTGAACCCGAAGAACTGATG
  GTGGACAACTGGCGCCCAGCTCAGCCACTGAAGAACAGGCAAATCAAAGCTTCCTTCAAA
  TAA
  
  Clone variation with respect to NM_000067.2
  562 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000067 unedited
  TTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCCCAAGCCGCCG
  CCGCCAGATCGGTGCCGATTCCTGCCCTGCCCCGACCGCCAGCGCGACCATGTCCCATCA
  CTGGGGGTACGGCAAACACAACGGACCTGAGCACTGGCATAAGGACTTCCCCATTGCCAA
  GGGAGAGCGCCAGTCCCCTGTTGACATCGACACTCATACAGCCAAGTATGACCCTTCCCT
  GAAGCCCCTGTCTGTTTCCTATGATCAAGCAACTTCCCTGAGGATCCTCAACAATGGTCA
  TGCTTTCAACGTGGAGTTTGATGACTCTCATGACAAAGCAGTGCTCAAGGGAGGACCCCT
  GGATGGCACTTACAGATTGATTCAGTTTCACTTTCACTGGGGTTCACTTGATGGACAAGG
  TTCAGAGCATACTGTGGATAAAAAGAAATATGCTGCAGAACTTCACTTGGTTCACTGGAA
  CACCAAATATGGGGATTTTGGGAAAGCTGTGCAGCAACCTGATGGACTGGCCGTTCTAAG
  TATTTTTTTGAAGGTTGGCAGCGCTAAACCGGGCCTTCAGAAAGTTGTTGATGTGCTGGA
  TTCCATTAAAACAAAGGGCAAGAGTGCTGACTTCACTAACTTCGATCCTCGAGGCCTCCT
  TACTGAATCCCTGGATTACTGGACCTACCCAGGCTCACTGACCACCCCTCCTCTTCTGGA
  ATGTGTGACCTGGATTGTGCTCAAGGAACCCATCAGCGTCAGCAGCGAGCAGGTGTTGAA
  ATTCCCTAAACTTAACTCAATGGGGAGGGTGAACCCGAAGACTGATGTGGGACAACTGCG
  CCCAGCTCANCCACTGAAGACAAGCAAATCAAAGCTTCCTTAAATAAGATGGCCCCTAGT
  CTGTAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000067 unedited
  ACCGCGGCCGCTATCTAGNATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTAAAATTCTGAATTTTTTTAAAAAATTTTTTTAAA
  ATCCAATTTTTTTTAAGTTTTGATTTTTTGGCTGAAGGGAAATATACCCTGTTTCACAAC
  TTTAATTTTAACAATTTTTACAAAAAAAACTTCTTTTTTTTCTTTGGAATTCAAAAGGCT
  TTAAATTCCCCCAAAATTCATTTTTAAAAAAACTTTGCTTTAAAAATAAAAAAATTTTTA
  AAGGTTTTTTTTAATAAAACATCTTGAAATCATGAACATTTTTTTTTTTGGGGTTCCCTT
  ACATAAAATCATGACTTTTTTCCCCGGTTTGCCTTAACTAACCCAATTTATTTTGGGCTT
  TGGATTTGCCCCCAACCATTTTACTTTCCTTTGTTTTAAAAACCCCTTTTTAATCAACAA
  TTGGGAGGGCTCATCACCCTACATGCTGGGAACAAAGGCAAGGGCCCTTATTCTTATTTC
  TTAACCCAAATTCGGGGGTCCAAAAAAACTCCCCCCCTAAACAAAGCCCCCCCCCCCCCC
  CCCAGTTGGGGCAAACATGACAATGGGGCTAACCTTCCATTTTATTAAAAAGCCTTTCAA
  GGAAAAAATGAAGGGGCTTAAAAACTTAACCCAAAGCCCCCCGGGGCCAAATCACCAAGG
  GAAATCTGGGCTTACCTAAAGGGAAACCCCCGAAAATTCTTATTTGGATACCGACTATGG
  GCACATTTTATTTGAAGGAACCTGGATTTGCCTGTTNCTTCAGGGCTGACCTGGCCGCAG
  TTTGTCACAATAATTTCTCGGGTCCCCCTCCCTTGAATTAAGTTACGGATTTCACCCTGC
  TCCTGTGACCTGAAGGTCCCTGACCCATCAGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000067
• Insert Size: 1750 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000067.1, NP_000058.1
• RefSeq Size: 1551 bp
• RefSeq ORF: 783 bp
• Locus ID: 760
• Cytogenetics: 8q21.2
• Domains: carb_anhydrase
• Protein Families: Druggable Genome
• Protein Pathways: Nitrogen metabolism
• Gene Summary: 'The protein encoded by this gene is one of several isozymes of carbonic anhydrase, which catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2014]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).