AP2B1 (NM_001282) Human Untagged Clone

CAT#: SC107907

AP2B1 (untagged)-Human adaptor-related protein complex 2, beta 1 subunit (AP2B1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: AP2B1
• Synonyms: ADTB2; AP2-BETA; AP105B; CLAPB1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC107907 sequence for NM_001282 edited (data generated by NextGen Sequencing)
  ATGACTGACTCCAAGTATTTCACAACCAATAAAAAAGGAGAAATATTTGAACTAAAAGCT
  GAACTCAACAATGAAAAGAAAGAAAAGAGAAAGGAGGCTGTGAAGAAAGTGATTGCTGCT
  ATGACCGTGGGGAAGGATGTTAGTTCTCTCTTTCCAGACGTAGTGAACTGTATGCAGACT
  GACAATCTGGAACTAAAGAAGCTTGTGTATCTCTACTTGATGAACTACGCCAAGAGTCAG
  CCAGACATGGCCATCATGGCTGTAAACAGCTTTGTGAAGGACTGTGAAGATCCTAATCCT
  TTGATTCGAGCCTTGGCAGTCAGAACCATGGGGTGCATCCGGGTAGACAAAATTACAGAA
  TATCTCTGTGAGCCGCTCCGCAAGTGCTTGAAGGATGAGGATCCCTATGTTCGGAAAACA
  GCAGCAGTCTGCGTGGCAAAACTCCATGATATCAATGCCCAAATGGTGGAAGATCAGGGA
  TTTCTGGATTCTCTACGGGATCTCATAGCAGATTCAAATCCAATGGTGGTGGCTAATGCC
  GTAGCGGCATTATCTGAAATCAGTGAGTCTCACCCAAACAGCAACTTACTTGATCTGAAC
  CCACAGAACATTAATAAGCTGCTGACAGCCCTGAATGAATGCACTGAATGGGGCCAGATT
  TTCATCCTGGACTGCCTGTCTAATTACAACCCTAAAGATGATCGGGAGGCTCAGAGCATC
  TGTGAGCGGGTAACTCCCCGGCTATCCCATGCCAACTCAGCAGTGGTGCTTTCAGCGGTA
  AAAGTCCTAATGAAGTTTCTAGAATTGTTACCTAAGGATTCTGACTACTACAATATGCTG
  CTGAAGAAGTTAGCCCCTCCACTTGTCACTTTGCTGTCTGGGGAGCCAGAAGTGCAGTAT
  GTCGCCCTGAGGAACATCAACTTAATTGTCCAGAAAAGGCCTGAAATCTTGAAGCAGGAA
  ATCAAAGTCTTCTTTGTGAAGTACAATGATCCCATCTATGTTAAACTAGAGAAGTTGGAC
  ATCATGATTCGTTTGGCATCTCAAGCCAACATTGCTCAGGTTCTGGCAGAACTGAAAGAA
  TATGCTACAGAGGTGGATGTTGACTTTGTTCGAAAAGCTGTGCGGGCCATTGGACGGTGT
  GCCATCAAGGTGGAGCAATCTGCAGAGCGCTGTGTAAGCACATTGCTTGATCTAATCCAG
  ACCAAAGTGAATTATGTGGTCCAAGAAGCAATTGTTGTCATCAGGGACATCTTCCGCAAA
  TACCCCAACAAGTATGAAAGTATCATCGCCACTCTGTGTGAGAACTTAGACTCGCTGGAT
  GAGCCAGATGCTCGAGCAGCTATGATTTGGATTGTGGGAGAATATGCTGAAAGAATTGAC
  AATGCAGATGAGTTACTAGAAAGCTTCCTGGAGGGTTTTCACGATGAAAGCACCCAGGTG
  CAGCTCACTCTGCTTACTGCCATAGTGAAGCTGTTTCTCAAGAAACCATCAGAAACACAG
  GAGCTAGTCCAGCAGGTCTTGAGTTTGGCAACACAGGATTCTGATAATCCTGACCTTCGA
  GACCGGGGCTATATTTATTGGCGCCTTCTCTCAACTGACCCTGTTACAGCTAAAGAAGTA
  GTCTTGTCTGAGAAGCCACTGATCTCTGAGGAGACGGACCTTATTGAGCCAACTCTGCTG
  GATGAGCTAATCTGCCACATTGGTTCTTTGGCCTCTGTGTATCATAAGCCTCCCAATGCT
  TTTGTGGAAGGAAGTCATGGAATTCATCGTAAACACTTGCCAATTCATCATGGGAGCACT
  GATGCAGGTGACAGCCCTGTTGGCACTACCACTGCAACGAACCTGGAACAGCCTCAGGTT
  ATCCCCTCTCAAGGTGATCTTCTAGGGGATCTTTTAAACCTTGACCTCGGTCCCCCAGTC
  AATGTGCCACAGGTGTCCTCCATGCAGATGGGAGCAGTGGATCTCCTAGGAGGAGGACTA
  GATAGTCTGGTGGGACAATCCTTCATCCCATCATCGGTCCCTGCAACCTTTGCTCCTTCA
  CCTACACCTGCTGTGGTCAGCAGTGGACTGAATGACCTGTTTGAACTCTCCACAGGGATA
  GGCATGGCACCTGGTGGATATGTGGCTCCTAAGGCTGTCTGGCTACCTGCAGTAAAGGCT
  AAAGGCTTGGAGATTTCCGGAACATTTACTCACCGCCAAGGGCACATCTATATGGAAATG
  AACTTCACCAATAAAGCTCTGCAGCACATGACAGATTTTGCAATCCAGTTTAACAAAAAT
  AGCTTTGGTGTCATCCCCAGCACTCCTCTGGCCATCCATACACCACTGATGCCAAACCAG
  AGCATTGATGTCTCCCTGCCTCTCAATACCTTGGGCCCAGTCATGAAGATGGAACCTCTG
  AATAACCTCCAGGTGGCTGTGAAAAACAATATCGATGTCTTCTACTTCAGCTGCCTCATC
  CCACTCAATGTGCTTTTTGTAGAAGATGGCAAAATGGAGCGCCAGGTCTTCCTTGCAACA
  TGGAAGGATATTCCCAATGAAAATGAACTTCAGTTTCAGATTAAGGAATGTCATTTAAAT
  GCTGACACTGTTTCCAGCAAGTTGCAAAACAACAATGTTTATACTATTGCCAAGAGGAAT
  GTGGAAGGGCAGGACATGCTGTACCAATCCCTGAAGCTCACTAATGGCATTTGGATTTTG
  GCCGAACTACGTATCCAGCCAGGAAACCCCAATTACACGCTGTCACTGAAGTGTAGAGCT
  CCTGAAGTCTCTCAATACATCTATCAGGTCTACGACAGCATTTTGAAAAACTAA
  
  Clone variation with respect to NM_001282.2
  2019 g=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001282 unedited
  TGTTCACATTTGTATACGACTCATATAGGCGGCCGCGAAATTCGCACGAGGCATCTTTGT
  CCCTGGCAAAGTGGGTTTTGCGCAGTGGCTTAGACCTAGAAAAGAATCGTGACGGGCAGG
  AAACCATTACACCACCACCTGGGCTGTGCTCTCCGGCTCCCGCCGCCACCCCCGCCCTCG
  CCTTCGCCTCCGCCTCCGGTGCACATTAAAGATCCAAAGTCATGACTGACTCCAAGTATT
  TCACAACCAATAAAAAAGGAGAAATATTTGAACTAAAAGCTGAACTCAACAATGAAAAGA
  AAGAAAAGAGAAAGGAGGCTGTGAAGAAAGTGATTGCTGCTATGACCGTGGGGAAGGATG
  TTAGTTCTCTCTTTCCAGACGTAGTGAACTGTATGCAGACTGACAATCTGGAACTAAAGA
  AGCTTGTGTATCTCTACTTGATGAACTACGCCAAGAGTCAGCCAGACATGGCCATCATGG
  CTGTAAACAGCTTTGTGAAGGACTGTGAAGATCCTAATCCTTTGATTCGAGCCTTGGCAG
  TCAGAACCATGGGGTGCATCCGGGTAGACAAAATTACAGAATATCTCTGTGAGCCGCTCC
  GCAAGTGCTTGAAGGATGAGGATCCCTATGTTCGGAAAACAGCAGCAGTCTGCGTGGCAA
  AACTCCATGATATCAATGCCCAAATGGTGGAAGATCAGGGATTTCTGGATTCTCTACGGG
  ATCTCATAGCAGATTCAAATCCAATGGTGGTGGCTAATGCCGTAGCGGCATTATCTGAAA
  TCAGTGAGTCTCACCCAAACAGCAACTTACTTGATCTGAACCCACAGAACATTAATAAGC
  TGCTGACAGCCCTGAATGAATGCACTGAATGGNGCCAGATTTTCATCCTGGACTGCCTGT
  CTATTACAACCCTAAGATGATCGGAGGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001282 unedited
  CGCGGCCGCAATCTAGAGTCGAGTTNTTTTTTTTTTTTTTTTTTTTTTTTTTTCCAGTAA
  CACTTTTCCTTGCTTTAATATTTGTTGCCTTTTTCTGCTTTTGAAATGAGCTCTCAATTA
  CAAAATACCAAACTACACTCAAAAGAGGGTGTAAAAAAAGCCAGCCATTGGCACAGCATG
  GAAAAGCCCACAAGTAAGGACAGATAGCAGCAGCAGGTGGCAAGAGGTGGGAGGTGACTG
  AAGCAGTGGTTACAAATGTTCACAGGAAGCTAAAACTATCCAACAGGTTGTGCCCTAATG
  TTAGCACAGGTTAAACTAGTTACTACCTTGCTAGGGGGCCTCAGTGTGTTCAGATTCTAC
  GCAGCAATACAATTTCTTCCAGTTAAGAGTTCTTGACTTGCACCGATCACAGCATGGTTG
  AAGGGTACTGGACCAGTCTTGTTAGTTTTTCAAAATGCTGTCGTAGACCTGATAGATGTA
  TTGAGAGACTTCAGGAGCTCTACACTTCAGTGACAGCGTGTAATTGGGGTTTCCTGGCTG
  GATACGTAGTTCGGCCAAAATCCAAATGCCATTAGTGAGCCTTCAGGAATTGGGACAGCA
  TGTCCTGCCCCTTCCACATTCCTTTAGGAAATAGAATAACATTGTTGTTTTGCAACTTGC
  TGAAACAGTGTCAGCATTTAAATGACATTCCTTATCGGAAACTGAAGTCATTTTCATTGG
  GCTTATCCTTCCATGTTGAAGGAAAAACCCGCGCTCATTTTTGCCTTTTTTACAAAAGCC
  CATTGTATGGGATGAGGCCCTTGAATAAAAAAACTCATATTGTTTTCCAAGCCCCCTTGA
  GGTTATTAAGAGTCCATTTTATGACTGGCCCAAGTATTGGGGAGCAGGAACATCATGCTC
  TGTTTGGATAATGGGTTATGATGCCCAAGAAGCTCGGGATACACAAACTTTTTGTAAACT
  GGTGGAAAATTGCTGGCTCGAATTTTATGGGAAACATTCCTAAAAGGCCTGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001282
• Insert Size: 3520 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001282.2, NP_001273.1
• RefSeq Size: 5730 bp
• RefSeq ORF: 2814 bp
• Locus ID: 163
• Cytogenetics: 17q12
• Domains: Armadillo_seg, Adaptin_N, Alpha_adaptinC2
• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis, Huntington's disease
• Gene Summary: 'The protein encoded by this gene is one of two large chain components of the assembly protein complex 2, which serves to link clathrin to receptors in coated vesicles. The encoded protein is found on the cytoplasmic face of coated vesicles in the plasma membrane. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) lacks an in-frame exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a.