ATP6V1A (NM_001690) Human Untagged Clone

CAT#: SC108263

ATP6V1A (untagged)-Human ATPase, H+ transporting, lysosomal 70kDa, V1 subunit A (ATP6V1A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ATP6V1A
• Synonyms: ARCL2D; ATP6A1; ATP6V1A1; HO68; IECEE3; VA68; Vma1; VPP2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC108263 sequence for NM_001690 edited (data generated by NextGen Sequencing)
  ATGGATTTTTCCAAGCTACCCAAAATACTCGATGAAGATAAAGAAAGCACATTTGGTTAT
  GTGCATGGGGTCTCAGGACCTGTGGTTACAGCCTGTGACATGGCGGGTGCAGCCATGTAT
  GAGCTGGTGAGAGTGGGCCACAGCGAATTGGTTGGAGAGATTATTCGATTGGAGGGTGAC
  ATGGCTACTATTCAGGTGTATGAAGAAACTTCTGGTGTGTCTGTTGGAGATCCTGTACTT
  CGCACTGGTAAACCCCTCTCTGTAGAGCTTGGTCCTGGCATTATGGGAGCCATTTTTGAT
  GGTATTCAAAGACCTTTGTCGGATATCAGCAGTCAGACCCAAAGCATCTACATCCCCAGA
  GGAGTAAACGTGTCTGCTCTTAGCAGAGATATCAAATGGGACTTTACACCTTGCAAAAAC
  CTACGGGTTGGTAGTCATATCACTGGCGGAGACATTTATGGAATTGTCAGTGAGAACTCG
  CTTATCAAACACAAAATCATGTTACCCCCACGAAACAGAGGAACTGTAACTTACATTGCT
  CCACCTGGGAATTATGATACCTCTGATGTTGTCTTGGAGCTTGAATTTGAAGGTGTAAAG
  GAGAAGTTCACCATGGTGCAAGTATGGCCTGTACGTCAAGTTCGACCTGTCACTGAGAAG
  CTGCCAGCCAATCATCCTCTGTTGACTGGCCAGAGAGTCCTTGATGCCCTTTTTCCGTGT
  GTCCAGGGAGGAACTACTGCTATCCCTGGAGCCTTTGGCTGTGGAAAGACAGTGATATCA
  CAGTCTCTATCCAAGTATTCTAACAGTGATGTAATCATCTATGTAGGATGTGGTGAAAGA
  GGAAATGAGATGTCTGAAGTCCTCCGGGACTTCCCAGAGCTCACAATGGAGGTTGATGGT
  AAGGTAGAGTCAATTATGAAGAGGACAGCTTTGGTAGCCAATACCTCCAATATGCCTGTT
  GCTGCTAGAGAAGCCTCTATTTATACTGGAATCACACTGTCAGAGTACTTCCGTGACATG
  GGCTATCATGTCAGTATGATGGCTGACTCTACCTCTAGATGGGCTGAGGCCCTTAGAGAA
  ATCTCTGGTCGTTTAGCTGAAATGCCTGCAGATAGTGGATATCCAGCCTATCTTGGTGCC
  CGTCTGGCCTCGTTTTATGAACGAGCAGGCAGGGTGAAATGTCTTGGAAATCCTGAAAGA
  GAAGGGAGTGTCAGCATTGTAGGAGCAGTTTCTCCACCTGGTGGTGATTTTTCTGATCCA
  GTTACATCTGCCACTCTTGGTATCGTTCAGGTGTTCTGGGGCTTAGATAAGAAACTAGCT
  CAACGTAAGCATTTCCCCTCTGTCAATTGGCTCATCAGCTACAGCAAGTATATGCGTGCC
  TTGGATGAATACTATGACAAACACTTCACAGAGTTCGTTCCTCTGAGGACGAAAGCTAAG
  GAAATTCTGCAGGAAGAAGAAGACCTGGCAGAAATTGTACAGCTTGTGGGAAAGGCTTCT
  TTGGCAGAAACAGATAAAATCACTCTGGAGGTAGCAAAACTTATCAAAGATGATTTCCTA
  CAACAAAATGGATATACTCCTTATGACAGGTTCTGCCCATTCTACAAGACAGTAGGGATG
  CTGTCCAACATGATTGCATTTTATGATATGGCTCGTAGAGCTGTTGAAACCACTGCCCAG
  AGTGACAATAAAATCACATGGTCCATTATTCGTGAGCACATGGGAGACATCCTCTATAAA
  CTTTCCTCCATGAAATTCAAGGATCCACTGAAAGATGGTGAGGCAAAGATCAAAAGCGAC
  TATGCACAACTTCTTGAAGACATGCAGAATGCATTCCGTAGCCTTGAAGATTAG
  
  Clone variation with respect to NM_001690.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001690 unedited
  NNNNGGGGGGGGCAGTACGNTTATTTGTCATACNACTCACTATAGGCGGCCGCGAATTCG
  CACGAGGCTGGGTCCTCGGTCGGTACAGTCTCTGCACCTCGCGCCCCAGCAGGTAAACTA
  ACATTATGGATTTTTCCAAGCTACCCAAAATACTCGATGAAGATAAAGAAAGCACATTTG
  GTTATGTGCATGGGGTCTCAGGACCTGTGGTTACAGCCTGTGACATGGCGGGTGCAGCCA
  TGTATGAGCTGGTGAGAGTGGGCCACAGCGAATTGGTTGGAGAGATTATTCGATTGGAGG
  GTGACATGGCTACTATTCAGGTGTATGAAGAAACTTCTGGTGTGTCTGTTGGAGATCCTG
  TACTTCGCACTGGTAAACCCCTCTCTGTAGAGCTTGGTCCTGGCATTATGGGAGCCATTT
  TTGATGGTATTCAAAGACCTTTTGTCGATATCAGCAGTCAGACCCAAAGCATCTACATCC
  NCAGAGGAGTAAACGTGTCTGCTCTTAGCAGAGATATCAAATGGGACTTTACACCTTGCA
  AAAACCTACGGNTTGGTAGTCATATCACTGGGCGAGACATTTATGGGAATTGTCAGTGAG
  AACTCCCTTATCAAACACAAAATCATGTTACCCCCACGAAACAGAGAAACTGGAACTTAC
  ATTGCTTCCACCTGGGAATTATGAAACCCTCTGATGTTGGCCTTGAAGCTTGGAATTGGA
  AGGGGTTAAAGGAAAAATTTACCCATGGGGGCAAGTTTGGCCCTTGACCTCAAAGTTCCA
  CCCGGTCATTAAAAAACTCCCAGCCCAATCTTCCTTTTGTTTAATGGGCCCAAAAATCCT
  TTGGGCCCCTTTTTCCCCGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001690 unedited
  ATGGACCGCGGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTGGAGGGTACTAT
  TTCATATATTGTGTGAGCCCCACAAATGTCTATTTTAAAAAGAGTATAGTCCCTGGCCAG
  GCGCGGTGGCTCACGCCTGTAATCCCAGCAGTTTGGGAGGCCGAGGTGGGCGGATCACCT
  GAGGTCTGGAGTTCGAGACCAGCCTGACCAATATGGTGAAACCCCGTTTCTACTAAAAAT
  ACAAAATTAGCTGGGCATGGTGGAGCATGCCTGTAATCCCAGCTACTCGGGAGGCTGAGG
  CAGGAGAATCACTTGAACCCGGGAGACGAAGGCTGCAGTGAGCCAAGATCACGCCATTGC
  ACTCCAGCCTGGGCAACAAGAGGGACACTCCGTCCCCAAAAAAAAATAATAAAAAAAATA
  AAAAATAAAAATAAAAAGAGTATAGTTCCCAATGGTTTCTCACAAACATTCCTGATTTAT
  ACTGGGGGAAGTGATGCATAATTGGAACATTAATCATTATGTTTTTGAAAATTAAATATT
  TCCTGCAAAGCAATTCCTTTGCAAAATGCTAAGCTGCCAATGAGCTTACCACAATAGAAA
  TTGAGTCATTTCACAAAGTACCTGTTCAAAAGTTATTAGAAACCTGGCCTTCCATTCCAA
  ATTCACTGCANAGAGAGTACATNGCAGTAGTCCTGTTTACAATTTAATAGAGATGAGGAA
  AGGGGTACACCTANGACAAAGAGGGGTCATCACCCCTACATACAGTNTACAGGTAGTGTA
  ACCAGAATGTATATTTACTATATTCACCACTCGAATAAAGGGAGAGGNAGGCCTTNCACA
  CACTAGACAAAAGAATTGTTGNTTATNTTAAGACCTACCAAAAACAGGGGAACAAATACC
  ACATTAGGCACTATNCTCAAGCTGACANTAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001690
• Insert Size: 2880 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001690.2, NP_001681.2
• RefSeq Size: 4567 bp
• RefSeq ORF: 1854 bp
• Locus ID: 523
• Cytogenetics: 3q13.31
• Domains: ATP-synt_ab, ATP-synt_ab_C, ATP-synt_ab_N
• Protein Families: Druggable Genome
• Protein Pathways: Epithelial cell signaling in Helicobacter pylori infection, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection
• Gene Summary: 'This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain A subunit isoforms and is found in all tissues. Transcript variants derived from alternative polyadenylation exist. [provided by RefSeq, Jul 2008]'