ATP6V1A (NM_001690) Human Untagged Clone

CAT#: SC108263

ATP6V1A (untagged)-Human ATPase, H+ transporting, lysosomal 70kDa, V1 subunit A (ATP6V1A)


  "NM_001690" in other vectors (6)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

Product Images

Other products for "ATP6V1A"

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol ATP6V1A
Synonyms ARCL2D; ATP6A1; ATP6V1A1; HO68; IECEE3; VA68; Vma1; VPP2
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC108263 sequence for NM_001690 edited (data generated by NextGen Sequencing)
ATGGATTTTTCCAAGCTACCCAAAATACTCGATGAAGATAAAGAAAGCACATTTGGTTAT
GTGCATGGGGTCTCAGGACCTGTGGTTACAGCCTGTGACATGGCGGGTGCAGCCATGTAT
GAGCTGGTGAGAGTGGGCCACAGCGAATTGGTTGGAGAGATTATTCGATTGGAGGGTGAC
ATGGCTACTATTCAGGTGTATGAAGAAACTTCTGGTGTGTCTGTTGGAGATCCTGTACTT
CGCACTGGTAAACCCCTCTCTGTAGAGCTTGGTCCTGGCATTATGGGAGCCATTTTTGAT
GGTATTCAAAGACCTTTGTCGGATATCAGCAGTCAGACCCAAAGCATCTACATCCCCAGA
GGAGTAAACGTGTCTGCTCTTAGCAGAGATATCAAATGGGACTTTACACCTTGCAAAAAC
CTACGGGTTGGTAGTCATATCACTGGCGGAGACATTTATGGAATTGTCAGTGAGAACTCG
CTTATCAAACACAAAATCATGTTACCCCCACGAAACAGAGGAACTGTAACTTACATTGCT
CCACCTGGGAATTATGATACCTCTGATGTTGTCTTGGAGCTTGAATTTGAAGGTGTAAAG
GAGAAGTTCACCATGGTGCAAGTATGGCCTGTACGTCAAGTTCGACCTGTCACTGAGAAG
CTGCCAGCCAATCATCCTCTGTTGACTGGCCAGAGAGTCCTTGATGCCCTTTTTCCGTGT
GTCCAGGGAGGAACTACTGCTATCCCTGGAGCCTTTGGCTGTGGAAAGACAGTGATATCA
CAGTCTCTATCCAAGTATTCTAACAGTGATGTAATCATCTATGTAGGATGTGGTGAAAGA
GGAAATGAGATGTCTGAAGTCCTCCGGGACTTCCCAGAGCTCACAATGGAGGTTGATGGT
AAGGTAGAGTCAATTATGAAGAGGACAGCTTTGGTAGCCAATACCTCCAATATGCCTGTT
GCTGCTAGAGAAGCCTCTATTTATACTGGAATCACACTGTCAGAGTACTTCCGTGACATG
GGCTATCATGTCAGTATGATGGCTGACTCTACCTCTAGATGGGCTGAGGCCCTTAGAGAA
ATCTCTGGTCGTTTAGCTGAAATGCCTGCAGATAGTGGATATCCAGCCTATCTTGGTGCC
CGTCTGGCCTCGTTTTATGAACGAGCAGGCAGGGTGAAATGTCTTGGAAATCCTGAAAGA
GAAGGGAGTGTCAGCATTGTAGGAGCAGTTTCTCCACCTGGTGGTGATTTTTCTGATCCA
GTTACATCTGCCACTCTTGGTATCGTTCAGGTGTTCTGGGGCTTAGATAAGAAACTAGCT
CAACGTAAGCATTTCCCCTCTGTCAATTGGCTCATCAGCTACAGCAAGTATATGCGTGCC
TTGGATGAATACTATGACAAACACTTCACAGAGTTCGTTCCTCTGAGGACGAAAGCTAAG
GAAATTCTGCAGGAAGAAGAAGACCTGGCAGAAATTGTACAGCTTGTGGGAAAGGCTTCT
TTGGCAGAAACAGATAAAATCACTCTGGAGGTAGCAAAACTTATCAAAGATGATTTCCTA
CAACAAAATGGATATACTCCTTATGACAGGTTCTGCCCATTCTACAAGACAGTAGGGATG
CTGTCCAACATGATTGCATTTTATGATATGGCTCGTAGAGCTGTTGAAACCACTGCCCAG
AGTGACAATAAAATCACATGGTCCATTATTCGTGAGCACATGGGAGACATCCTCTATAAA
CTTTCCTCCATGAAATTCAAGGATCCACTGAAAGATGGTGAGGCAAAGATCAAAAGCGAC
TATGCACAACTTCTTGAAGACATGCAGAATGCATTCCGTAGCCTTGAAGATTAG

Clone variation with respect to NM_001690.3
>OriGene 5' read for NM_001690 unedited
NNNNGGGGGGGGCAGTACGNTTATTTGTCATACNACTCACTATAGGCGGCCGCGAATTCG
CACGAGGCTGGGTCCTCGGTCGGTACAGTCTCTGCACCTCGCGCCCCAGCAGGTAAACTA
ACATTATGGATTTTTCCAAGCTACCCAAAATACTCGATGAAGATAAAGAAAGCACATTTG
GTTATGTGCATGGGGTCTCAGGACCTGTGGTTACAGCCTGTGACATGGCGGGTGCAGCCA
TGTATGAGCTGGTGAGAGTGGGCCACAGCGAATTGGTTGGAGAGATTATTCGATTGGAGG
GTGACATGGCTACTATTCAGGTGTATGAAGAAACTTCTGGTGTGTCTGTTGGAGATCCTG
TACTTCGCACTGGTAAACCCCTCTCTGTAGAGCTTGGTCCTGGCATTATGGGAGCCATTT
TTGATGGTATTCAAAGACCTTTTGTCGATATCAGCAGTCAGACCCAAAGCATCTACATCC
NCAGAGGAGTAAACGTGTCTGCTCTTAGCAGAGATATCAAATGGGACTTTACACCTTGCA
AAAACCTACGGNTTGGTAGTCATATCACTGGGCGAGACATTTATGGGAATTGTCAGTGAG
AACTCCCTTATCAAACACAAAATCATGTTACCCCCACGAAACAGAGAAACTGGAACTTAC
ATTGCTTCCACCTGGGAATTATGAAACCCTCTGATGTTGGCCTTGAAGCTTGGAATTGGA
AGGGGTTAAAGGAAAAATTTACCCATGGGGGCAAGTTTGGCCCTTGACCTCAAAGTTCCA
CCCGGTCATTAAAAAACTCCCAGCCCAATCTTCCTTTTGTTTAATGGGCCCAAAAATCCT
TTGGGCCCCTTTTTCCCCGG
>OriGene 3' read for NM_001690 unedited
ATGGACCGCGGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTGGAGGGTACTAT
TTCATATATTGTGTGAGCCCCACAAATGTCTATTTTAAAAAGAGTATAGTCCCTGGCCAG
GCGCGGTGGCTCACGCCTGTAATCCCAGCAGTTTGGGAGGCCGAGGTGGGCGGATCACCT
GAGGTCTGGAGTTCGAGACCAGCCTGACCAATATGGTGAAACCCCGTTTCTACTAAAAAT
ACAAAATTAGCTGGGCATGGTGGAGCATGCCTGTAATCCCAGCTACTCGGGAGGCTGAGG
CAGGAGAATCACTTGAACCCGGGAGACGAAGGCTGCAGTGAGCCAAGATCACGCCATTGC
ACTCCAGCCTGGGCAACAAGAGGGACACTCCGTCCCCAAAAAAAAATAATAAAAAAAATA
AAAAATAAAAATAAAAAGAGTATAGTTCCCAATGGTTTCTCACAAACATTCCTGATTTAT
ACTGGGGGAAGTGATGCATAATTGGAACATTAATCATTATGTTTTTGAAAATTAAATATT
TCCTGCAAAGCAATTCCTTTGCAAAATGCTAAGCTGCCAATGAGCTTACCACAATAGAAA
TTGAGTCATTTCACAAAGTACCTGTTCAAAAGTTATTAGAAACCTGGCCTTCCATTCCAA
ATTCACTGCANAGAGAGTACATNGCAGTAGTCCTGTTTACAATTTAATAGAGATGAGGAA
AGGGGTACACCTANGACAAAGAGGGGTCATCACCCCTACATACAGTNTACAGGTAGTGTA
ACCAGAATGTATATTTACTATATTCACCACTCGAATAAAGGGAGAGGNAGGCCTTNCACA
CACTAGACAAAAGAATTGTTGNTTATNTTAAGACCTACCAAAAACAGGGGAACAAATACC
ACATTAGGCACTATNCTCAAGCTGACANTAAA
Restriction Sites NotI-NotI     
ACCN NM_001690
Insert Size 2880 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001690.2, NP_001681.2
RefSeq Size 4567 bp
RefSeq ORF 1854 bp
Locus ID 523
Cytogenetics 3q13.31
Domains ATP-synt_ab, ATP-synt_ab_C, ATP-synt_ab_N
Protein Families Druggable Genome
Protein Pathways Epithelial cell signaling in Helicobacter pylori infection, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection
Gene Summary 'This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain A subunit isoforms and is found in all tissues. Transcript variants derived from alternative polyadenylation exist. [provided by RefSeq, Jul 2008]'

Other Versions

{0} Product Review(s)

0 Product Review(s) Submit review

Be the first one to submit a review

Product Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.