RBPJK (RBPJ) (NM_015874) Human Untagged Clone

CAT#: SC108442

RBPJ (untagged)-Human recombination signal binding protein for immunoglobulin kappa J region (RBPJ), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RBPJ
• Synonyms: AOS3; CBF1; csl; IGKJRB; IGKJRB1; KBF2; RBP-J; RBPJK; RBPSUH; SUH
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_015874 edited
  GAATTCGGCACGAGGGGAAGATGGCGCCTGTTGTGACAGGTCATTGAAATTATGAGACTA
  TCATTCAAATGGAAGCATTATAGTTCTTCGGAACCATTATGATCTCAAAACGAAAGGAGA
  ATGATACAGATACACTGGCTGAGGTGTTTTGAGGTGCATCGAAGTGTTCCAAGCTGTGAC
  TTACCTTAACATGTTCTTGAAGTACCATGGCGTGGATTAAAAGGAAATTTGGTGAGCGGC
  CTCCACCTAAACGACTTACTAGGGAAGCTATGCGAAATTATTTAAAAGAGCGAGGGGATC
  AAACAGTACTTATTCTTCATGCAAAAGTTGCACAGAAGTCATATGGAAATGAAAAAAGGT
  TTTTTTGCCCACCTCCTTGTGTATATCTTATGGGCAGTGGATGGAAGAAAAAAAAAGAAC
  AAATGGAACGCGATGGTTGTTCTGAACAAGAGTCTCAACCGTGTGCATTTATTGGGATAG
  GAAATAGTGACCAAGAAATGCAGCAGCTAAACTTGGAAGGAAAGAACTATTGCACAGCCA
  AAACATTGTATATATCTGACTCAGACAAGCGAAAGCACTTCATGTTGTCTGTAAAGATGT
  TCTATGGCAACAGTGATGACATTGGTGTGTTCCTCAGCAAGCGGATAAAAGTCATCTCCA
  AACCTTCCAAAAAGAAGCAGTCATTGAAAAATGCTGACTTATGCATTGCCTCAGGAACAA
  AGGTGGCTCTGTTTAATCGACTACGATCCCAGACAGTTAGTACCAGATACTTGCATGTAG
  AAGGAGGTAATTTTCATGCCAGTTCACAGCAGTGGGGAGCCTTTTTTATTCATCTCTTGG
  ATGATGATGAATCAGAAGGAGAAGAATTCACAGTCCGAGATGGCTACATCCATTATGGAC
  AAACAGTCAAACTTGTGTGCTCAGTTACTGGCATGGCACTCCCAAGATTGATAATTAGGA
  AAGTTGATAAGCAGACCGCATTATTGGATGCAGATGATCCTGTGTCACAACTCCATAAAT
  GTGCATTTTACCTTAAGGATACAGAAAGAATGTATTTGTGCCTTTCTCAAGAAAGAATAA
  TTCAATTTCAGGCCACTCCATGTCCAAAAGAACCAAATAAAGAGATGATAAATGATGGCG
  CTTCCTGGACAATCATTAGCACAGATAAGGCAGAGTATACATTTTATGAGGGAATGGGCC
  CTGTCCTTGCCCCAGTCACTCCTGTGCCTGTGGTAGAGAGCCTTCAGTTGAATGGCGGTG
  GGGACGTAGCAATGCTTGAACTTACAGGACAGAATTTCACTCCAAATTTACGAGTGTGGT
  TTGGGGATGTAGAAGCTGAAACTATGTACAGGTGTGGAGAGAGTATGCTCTGTGTCGTCC
  CAGACATTTCTGCATTCCGAGAAGGTTGGAGATGGGTCCGGCAACCAGTCCAGGTTCCAG
  TAACTTTGGTCCGAAATGATGGAATCATTTATTCCACCAGCCTTACCTTTACCTACACAC
  CAGAACCAGGGCCGCGGCCACATTGCAGTGCAGCAGGAGCAATCCTTCGAGCCAATTCAA
  GCCAGGTGCCCCCTAACGAATCAAACACAAACAGCGAGGGAAGTTACACAAACGCCAGCA
  CAAATTCAACCAGTGTCACATCATCTACAGCCACAGTGGTATCCTAACTACCGTCTTTTT
  GCTAGGACTTAAACTGACTTGAGTGTGGCAAAAAGTTAACAAAAAAGGAGAAAAAATGAA
  CAATCGTTTGTGGTTTCTTGGGAAAACTTTTCATACCAGGTGATACTATTCAAAAACCCC
  GTTGTCTCCCTGCAAGTGCTGATTTGAAATGCAGAAGCCACAGTAAAAAAAAAAAAAAAA
  AACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_015874 unedited
  AGCATTTTGTAATACGACTCACTATAGGCGGCNCGCGAATTCGCACGAGGGAAGAGGCGC
  CTGTTGTGACAGGTCATTGAAATTATGAGACTATCATTCAAATGGAAGCATTATAGTTCT
  TCGGAACCATTATGATCTCAAAACGAAAGGAGAATGATACAGATACACTGGCTGAGGTGT
  TTTGAGGTGCATCGAAGTGTTCCAAGCTGTGACTTACCTTAACATGTTCTTGAAGTACCA
  TGGCGTGGATTAAAAGGAAATTTGGTGAGCGGCCTCCACCTAAACGACTTACTAGGGAAG
  CTATGCGAAATTATTTAAAAGAGCGAGGGGATCAAACAGTACTTATTCTTCATGCAAAAG
  TTGCACAGAAGTCATATGGAAATGAAAAAAGGTTTTTTTGCCCACCTCCTTGTGTATATC
  TTATGGGCAGTGGATGGAAGAAAAAAAAAGAACAAATGGAACGCGATGGTTGTTCTGAAC
  AAGAGTCTCAACCGTGTGCATTTATTGGGATAGGAAATAGTGACCAAGAAATGCAGCAGC
  TAAACTTGGAAGGAAAGAACTATTGCACAGCCAAAACATTGTATATATCTGACTCAGACA
  AGCGAAAGCACTTCATGTTGTCTGTAAAGATGTTCTATGGCAACAGTGATGACATTGGTG
  TGTTCCTCAGCAAGCGGATAAAAGTCATCTCCAAACCTTCCAAAAAGAAGCAGTCATTGA
  AAAATGCTGACTTATGCATTGCCTCAGGAACANAGGTGGCTCTGTTTAATCGACTACGAT
  CCCAGACAGTTAGTACCAGATACTTGCATGTAGAAGGAGGTAATTNTCATGCCAGTTCAC
  AGCAGTGGNGAGCCTTTTTTATTCATCTCTTGNATGATGATGAATCAGAAAGAGAGCAAT
  CACAGTCCGAGATGGCTACATCCATTATGGNACAACAAGTCAACTTGTGTGCTCAGNTAC
  TGGCATGGCACTCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_015874 unedited
  CCCCCCCATTCGTGTGNNCCCGCGCGCCGCAATTCTACGATCGAGTTTTTTTTTTTTTTT
  TTTACTGTGGCTTCTGTTTTTCAAATCAGCACTTGCAGGGAGACAACGGGGTTTTTGAAT
  AGTATCACCTGGTATGAAAAGTTTTCCCAAGAAACCACAAACGATTGTTCATTTTTTCTC
  CTTTTTTGTTAACTTTTTGCCACACTCAAGTCAGTTTAAGTCCTAGCAAAAAGACGGTAG
  TTAGGATACCACTGTGGCTGTAGATGATGTGACACTGGTTGAATTTGTGCTGGCGTTTGT
  GTAACTTCCCTCGCTGTTTGTGTTTGATTCGTTAGGGGGCACCTGGCTTGAATTGGCTCG
  AAGGATTGCTCCTGCTGCACTGCAATGTGGCCGCGGCCCTGGTTCTGGTGTGTAGGTAAA
  GGTAAGGCTGGTGGAATAAATGATTCCATCATTTCGGACCAAAGTTACTGGAACCTGGAC
  TGGTTGCCGGACCCATCTCCAACCTTCTCGGAATGCAGAAATGTCTGGGACGACACAGAG
  CATACTCTCTCCACACCTGTACATAGTTTCAGCTTCTACATCCCCAAACCACACTCGTAA
  ATTTGGAGTGAAATTCTGTCCTGTAAGTTCAAGCATTGCTACGTCCCCACCGCCATTCCA
  CTGAAGGCTCTCTACCACCAGGCACAGGAGTGACTGGGGCAAGGACAGGGCCCATTCCCT
  CACAAAATGAATACTCTGCCTTATCTGCGCTAATGATTGCCCAGGAAGCGCCATCATTTA
  TCACCCCTATATTCTGGTTCTTTTGCACATGGAGTGCCCTGAAAATGAATTATTCTTTTT
  TTGAGAAGGACAAATACCTTCTTTCTGCATCCTTAGGTAAAATGCACATTATGCATTGTG
  ACCAGGAACAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_015874
• Insert Size: 1700 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015874.3, NP_056958.3
• RefSeq Size: 2316 bp
• RefSeq ORF: 1464 bp
• Locus ID: 3516
• Cytogenetics: 4p15.2
• Protein Families: Transcription Factors
• Protein Pathways: Notch signaling pathway
• Gene Summary: 'The protein encoded by this gene is a transcriptional regulator important in the Notch signaling pathway. The encoded protein acts as a repressor when not bound to Notch proteins and an activator when bound to Notch proteins. It is thought to function by recruiting chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins to Notch signaling pathway genes. Several transcript variants encoding different isoforms have been found for this gene, and several pseudogenes of this gene exist on chromosome 9. [provided by RefSeq, Oct 2013]'
  Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.