NEK2 (NM_002497) Human Untagged Clone

CAT#: SC108482

NEK2 (untagged)-Human NIMA (never in mitosis gene a)-related kinase 2 (NEK2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NEK2
• Synonyms: HsPK21; NEK2A; NLK1; PPP1R111; RP67
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_002497 edited
  ATGCCTTCCCGGGCGGAGGACTATGAAGTGTTGTACACCATTGGCACAGGCTCCTACGGC
  CGCTGCCAGAAGATCCGGAGGAAGAGTGATGGCAAGATATTAGTTTGGAAAGAACTTGAC
  TATGGCTCCATGACAGAAGCTGAGAAACAGATGCTTGTTTCTGAAGTGAATTTGCTTCGT
  GAACTGAAACATCCAAACATCGTTCGTTACTATGATCGGATTATTGACCGGACCAATACA
  ACACTGTACATTGTAATGGAATATTGTGAAGGAGGGGATCTGGCTAGTGTAATTACAAAG
  GGAACCAAGGAAAGGCAATACTTAGATGAAGAGTTTGTTCTTCGAGTGATGACTCAGTTG
  ACTCTGGCCCTGAAGGAATGCCACAGACGAAGTGATGGTGGTCATACCGTATTGCATCGG
  GATCTTAAACCAGCCAATGTTTTCCTGGATGGCAAGCAAAACGTCAAGCTTGGAGACTTT
  GGGCTAGCTAGAATATTAAACCATGACACGAGTTTTGCAAAAACATTTGTTGGCACACCT
  TATTACATGTCTCCTGAACAAATGAATCGCATGTCCTACAATGAGAAATCAGATATCTGG
  TCATTGGGCTGCTTGCTGTATGAGTTATGTGCATTAATGCCTCCATTTACAGCTTTTAGC
  CAGAAAGAACTCGCTGGGAAAATCAGAGAAGGCAAATTCAGGCGAATTCCATACCGTTAC
  TCTGATGAATTGAATGAAATTATTACGAGGATGTTAAACTTAAAGGATTACCATCGACCT
  TCTGTTGAAGAAATTCTTGAGAACCCTTTAATAGCAGATTTGGTTGCAGACGAGCAAAGA
  AGAAATCTTGAGAGAAGAGGGCGACAATTAGGAGAGCCAGAAAAATCGCAGGATTCCAGC
  CCTGTATTGAGTGAGCTGAAACTGAAGGAAATTCAGTTACAGGAGCGAGAGCGAGCTCTC
  AAAGCAAGAGAAGAAAGATTGGAGCAGAAAGAACAGGAGCTTTGTGTTCGTGAGAGACTA
  GCAGAGGACAAACTGGCTAGAGCAGAAAATCTGTTGAAGAACTACAGCTTGCTAAAGGAA
  CGGAAGTTCCTGTCTCTGGCAAGTAATCCAGAACTTCTTAATCTTCCATCCTCAGTAATT
  AAGAAGAAAGTTCATTTCAGTGGGGAAAGTAAAGAGAACATCATGAGGAGTGAGAATTCT
  GAGAGTCAGCTCACATCTAAGTCCAAGTGCAAGGACCTGAAGAAAAGGCTTCACGCTGCC
  CAGCTGCGTGCTCAAGCCCTGTCAGATATTGAGAAAAATTACCAACTGAAAAGCAGGCAG
  ATCCTGGGCATGCGCTAG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002497 unedited
  GGAGCCTCCATTTTGTAATACGACTTACTATAGGCGGCACGCGAATTCGCACCAGGCGGG
  TCAGTGCTGCTCGGGGGCTTCTCCATCCAGGTCCCTGGAGTTCCTGGTCCCTGGAGCTCC
  GCACTTGGCGGCGCAACCTGCGTGAGGCAGCGCGACTCTGGCGACTGGCCGGCCATGCCT
  TCCCGGGCGGAGGACTATGAAGTGTTGTACACCATTGGCACAGGCTCCTACGGCCGCTGC
  CAGAAGATCCGGAGGAAGAGTGATGGCAAGATATTAGTTTGGAAAGAACTTGACTATGGC
  TCCATGACAGAAGCTGAGAAACAGATGCTTGTTTCTGAAGTGAATTTGCTTCGTGAACTG
  AAACATCCAAACATCGTTCGTTACTATGATCGGATTATTGACCGGACCAATACAACACTG
  TACATTGTAATGGAATATTGTGAAGGAGGGGATCTGGCTAGTGTAATTACAAAGGGAACC
  AAGGAAAGGCAATACTTAGATGAAGAGTTTGTTCTTCGAGTGATGACTCAGTTGACTCTG
  GCCCTGAAGGAATGCCACAGACGAAGTGATGGTGGTCATACCGTATTGCATCGGGATCTG
  AAACCAGCCAATGTTTTCCTGGATGGCAAGCAAAACGTCAAGCTTGGAGACTTTGGGCTA
  GCTAGAATATTAAACCACGACACGAGTTTTGCAAAAACATTTGTTGGCACACCTTATTAC
  ATGTCTCCTGAACAAATGAATCGCATGTCCTANCATGAGAAATCAGATATCTGGTCATTG
  GGCTGCTTGCTGTATGAGTTATGTGCATTAATGCCTCCATTTACAGNNCTTTAGCCAGAA
  GAACTCGCTGGGGAAATCAGAGAAGGCAAATTCAGGCGAATTCCATACCGTTACTCTGAT
  GAATTGAATGAATT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002497 unedited
  GGGGGAGCCCGGGCCCACCCCCCAAATTTTTANNNNNNNNNGTACGTTGNNACCGCGNNC
  CGCTTTCTNANNGATCGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACTG
  AAAAAATCTTTTATTTAGGGGGAAAGAAGTGGTAAATGACAGACAGCTCATATTCTGTTA
  AACAGAAAAAAAAAAGATACAAACATCACAGGGATGAATTTTCACAAAGCTAACAGATTT
  GAACTACAGAGCAATGGAATATTTATAAGCAAGATGTCATGGTATTAATGACCAAATTGC
  ATCTAACTGGGTTTTCTAAGCTCAAAAACATTTAAAATCTCAAACTTAAAATTTAAATCT
  AGACATGACAATTGTAAGCATACCACTCAGTCATTTAAAACTATTCAGGGAGGGCTACTC
  TTCCTGCATAAATATTTTTTTCCTAACATTATTTTTTCTCCCAAGATTTAAAATGTCACA
  TCTCATGTTCCTACTAGTAATCACACACAGGATTAAAAGCCCAACCAAGAAAGTATTCTT
  TTTATAATATGTTCTTAAAAGAAGAAAGAAAAATTAAATGTGAACATTTTGTACAATAGT
  TGCTGAAGAACAGTAAAACCAATTCCGAAATATCATGTGTACTATACAGAAAGGCATGGC
  TCATGGAACCAAGTATTCAACACTACAGCATTTGAATATCAGTCTTTAAAGGTTGGTAAT
  ATTACATCCTGTACACAGCTCTGTGTCTCTCTACCTGGCTAGCGCATGCCCAGGATCTGC
  CTGCTTTTCATTTGGAATTTTTCTAAAATCTGACAGGCTTGACCCGCACCTGGCACGTGA
  AGCCTTTTCTCAGGCCCTTGCCTTGGACTTAAAGTGACCTGACTCCAAAATCTCCTCCTC
  AGAAGTCCCTTACTTCCCCACGAAAGAACTTCTCTTAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002497
• Insert Size: 2210 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002497.2, NP_002488.1
• RefSeq Size: 2130 bp
• RefSeq ORF: 1338 bp
• Locus ID: 4751
• Cytogenetics: 1q32.3
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: 'This gene encodes a serine/threonine-protein kinase that is involved in mitotic regulation. This protein is localized to the centrosome, and undetectable during G1 phase, but accumulates progressively throughout the S phase, reaching maximal levels in late G2 phase. Alternatively spliced transcript variants encoding different isoforms with distinct C-termini have been noted for this gene. [provided by RefSeq, Feb 2011]'
  Transcript Variant: This variant (1) represents the predominant transcript, and encodes the longest isoform (1, also known as NEK2A). The C-terminus (aa 399-445) of this isoform was shown to be responsible for its nucleolar localization (PMID:15161910). Isoforms 1 and 2 (which differ at the C-terminus) have also been reported to exhibit distinct pattern of expression during mitosis (PMID:11742531).