NEK2 (NM_002497) Human Untagged Clone

CAT#: SC108482

NEK2 (untagged)-Human NIMA (never in mitosis gene a)-related kinase 2 (NEK2), transcript variant 1


  "NM_002497" in other vectors (7)

Reconstitution Protocol

USD 310.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol NEK2
Synonyms HsPK21; NEK2A; NLK1; PPP1R111; RP67
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF sequence for NM_002497 edited
ATGCCTTCCCGGGCGGAGGACTATGAAGTGTTGTACACCATTGGCACAGGCTCCTACGGC
CGCTGCCAGAAGATCCGGAGGAAGAGTGATGGCAAGATATTAGTTTGGAAAGAACTTGAC
TATGGCTCCATGACAGAAGCTGAGAAACAGATGCTTGTTTCTGAAGTGAATTTGCTTCGT
GAACTGAAACATCCAAACATCGTTCGTTACTATGATCGGATTATTGACCGGACCAATACA
ACACTGTACATTGTAATGGAATATTGTGAAGGAGGGGATCTGGCTAGTGTAATTACAAAG
GGAACCAAGGAAAGGCAATACTTAGATGAAGAGTTTGTTCTTCGAGTGATGACTCAGTTG
ACTCTGGCCCTGAAGGAATGCCACAGACGAAGTGATGGTGGTCATACCGTATTGCATCGG
GATCTTAAACCAGCCAATGTTTTCCTGGATGGCAAGCAAAACGTCAAGCTTGGAGACTTT
GGGCTAGCTAGAATATTAAACCATGACACGAGTTTTGCAAAAACATTTGTTGGCACACCT
TATTACATGTCTCCTGAACAAATGAATCGCATGTCCTACAATGAGAAATCAGATATCTGG
TCATTGGGCTGCTTGCTGTATGAGTTATGTGCATTAATGCCTCCATTTACAGCTTTTAGC
CAGAAAGAACTCGCTGGGAAAATCAGAGAAGGCAAATTCAGGCGAATTCCATACCGTTAC
TCTGATGAATTGAATGAAATTATTACGAGGATGTTAAACTTAAAGGATTACCATCGACCT
TCTGTTGAAGAAATTCTTGAGAACCCTTTAATAGCAGATTTGGTTGCAGACGAGCAAAGA
AGAAATCTTGAGAGAAGAGGGCGACAATTAGGAGAGCCAGAAAAATCGCAGGATTCCAGC
CCTGTATTGAGTGAGCTGAAACTGAAGGAAATTCAGTTACAGGAGCGAGAGCGAGCTCTC
AAAGCAAGAGAAGAAAGATTGGAGCAGAAAGAACAGGAGCTTTGTGTTCGTGAGAGACTA
GCAGAGGACAAACTGGCTAGAGCAGAAAATCTGTTGAAGAACTACAGCTTGCTAAAGGAA
CGGAAGTTCCTGTCTCTGGCAAGTAATCCAGAACTTCTTAATCTTCCATCCTCAGTAATT
AAGAAGAAAGTTCATTTCAGTGGGGAAAGTAAAGAGAACATCATGAGGAGTGAGAATTCT
GAGAGTCAGCTCACATCTAAGTCCAAGTGCAAGGACCTGAAGAAAAGGCTTCACGCTGCC
CAGCTGCGTGCTCAAGCCCTGTCAGATATTGAGAAAAATTACCAACTGAAAAGCAGGCAG
ATCCTGGGCATGCGCTAG
>OriGene 5' read for NM_002497 unedited
GGAGCCTCCATTTTGTAATACGACTTACTATAGGCGGCACGCGAATTCGCACCAGGCGGG
TCAGTGCTGCTCGGGGGCTTCTCCATCCAGGTCCCTGGAGTTCCTGGTCCCTGGAGCTCC
GCACTTGGCGGCGCAACCTGCGTGAGGCAGCGCGACTCTGGCGACTGGCCGGCCATGCCT
TCCCGGGCGGAGGACTATGAAGTGTTGTACACCATTGGCACAGGCTCCTACGGCCGCTGC
CAGAAGATCCGGAGGAAGAGTGATGGCAAGATATTAGTTTGGAAAGAACTTGACTATGGC
TCCATGACAGAAGCTGAGAAACAGATGCTTGTTTCTGAAGTGAATTTGCTTCGTGAACTG
AAACATCCAAACATCGTTCGTTACTATGATCGGATTATTGACCGGACCAATACAACACTG
TACATTGTAATGGAATATTGTGAAGGAGGGGATCTGGCTAGTGTAATTACAAAGGGAACC
AAGGAAAGGCAATACTTAGATGAAGAGTTTGTTCTTCGAGTGATGACTCAGTTGACTCTG
GCCCTGAAGGAATGCCACAGACGAAGTGATGGTGGTCATACCGTATTGCATCGGGATCTG
AAACCAGCCAATGTTTTCCTGGATGGCAAGCAAAACGTCAAGCTTGGAGACTTTGGGCTA
GCTAGAATATTAAACCACGACACGAGTTTTGCAAAAACATTTGTTGGCACACCTTATTAC
ATGTCTCCTGAACAAATGAATCGCATGTCCTANCATGAGAAATCAGATATCTGGTCATTG
GGCTGCTTGCTGTATGAGTTATGTGCATTAATGCCTCCATTTACAGNNCTTTAGCCAGAA
GAACTCGCTGGGGAAATCAGAGAAGGCAAATTCAGGCGAATTCCATACCGTTACTCTGAT
GAATTGAATGAATT
>OriGene 3' read for NM_002497 unedited
GGGGGAGCCCGGGCCCACCCCCCAAATTTTTANNNNNNNNNGTACGTTGNNACCGCGNNC
CGCTTTCTNANNGATCGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACTG
AAAAAATCTTTTATTTAGGGGGAAAGAAGTGGTAAATGACAGACAGCTCATATTCTGTTA
AACAGAAAAAAAAAAGATACAAACATCACAGGGATGAATTTTCACAAAGCTAACAGATTT
GAACTACAGAGCAATGGAATATTTATAAGCAAGATGTCATGGTATTAATGACCAAATTGC
ATCTAACTGGGTTTTCTAAGCTCAAAAACATTTAAAATCTCAAACTTAAAATTTAAATCT
AGACATGACAATTGTAAGCATACCACTCAGTCATTTAAAACTATTCAGGGAGGGCTACTC
TTCCTGCATAAATATTTTTTTCCTAACATTATTTTTTCTCCCAAGATTTAAAATGTCACA
TCTCATGTTCCTACTAGTAATCACACACAGGATTAAAAGCCCAACCAAGAAAGTATTCTT
TTTATAATATGTTCTTAAAAGAAGAAAGAAAAATTAAATGTGAACATTTTGTACAATAGT
TGCTGAAGAACAGTAAAACCAATTCCGAAATATCATGTGTACTATACAGAAAGGCATGGC
TCATGGAACCAAGTATTCAACACTACAGCATTTGAATATCAGTCTTTAAAGGTTGGTAAT
ATTACATCCTGTACACAGCTCTGTGTCTCTCTACCTGGCTAGCGCATGCCCAGGATCTGC
CTGCTTTTCATTTGGAATTTTTCTAAAATCTGACAGGCTTGACCCGCACCTGGCACGTGA
AGCCTTTTCTCAGGCCCTTGCCTTGGACTTAAAGTGACCTGACTCCAAAATCTCCTCCTC
AGAAGTCCCTTACTTCCCCACGAAAGAACTTCTCTTAA
Restriction Sites NotI-NotI     
ACCN NM_002497
Insert Size 2210 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002497.2, NP_002488.1
RefSeq Size 2130 bp
RefSeq ORF 1338 bp
Locus ID 4751
Cytogenetics 1q32.3
Domains pkinase, TyrKc, S_TKc
Protein Families Druggable Genome, Protein Kinase
Gene Summary 'This gene encodes a serine/threonine-protein kinase that is involved in mitotic regulation. This protein is localized to the centrosome, and undetectable during G1 phase, but accumulates progressively throughout the S phase, reaching maximal levels in late G2 phase. Alternatively spliced transcript variants encoding different isoforms with distinct C-termini have been noted for this gene. [provided by RefSeq, Feb 2011]'
Transcript Variant: This variant (1) represents the predominant transcript, and encodes the longest isoform (1, also known as NEK2A). The C-terminus (aa 399-445) of this isoform was shown to be responsible for its nucleolar localization (PMID:15161910). Isoforms 1 and 2 (which differ at the C-terminus) have also been reported to exhibit distinct pattern of expression during mitosis (PMID:11742531).

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